Gene description for XRCC5
Gene name X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining)
Gene symbol XRCC5
Other names/aliases KARP-1
KARP1
KU80
KUB2
Ku86
NFIV
Species Homo sapiens
 Database cross references - XRCC5
ExoCarta ExoCarta_7520
Vesiclepedia VP_7520
Entrez Gene 7520
HGNC 12833
MIM 194364
UniProt P13010  
 XRCC5 identified in sEVs derived from the following tissue/cell type
Bladder cancer cells 20224111    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 35918900    
Breast cancer cells 35918900    
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Colorectal cancer cells 34887515    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 36706192    
Embryonic kidney cells 36706192    
Foreskin fibroblasts 34108659    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Lymphoma cells 34108659    
Mammary cancer-associated fibroblasts 34108659    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Mesenchymal stem cells 36408942    
Monocytic leukemia cells 34108659    
Normal mammary epithelial cells 34108659    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic duct epithalial cells 34108659    
Pancreatic duct epithalial cells 34108659    
Pluripotent stem cells 34108659    
Prostate cancer cells 22723089    
T lymphocytes 34108659    
Thymus 23844026    
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for XRCC5
Molecular Function
    transcription cis-regulatory region binding GO:0000976 IDA
    DNA binding GO:0003677 NAS
    DNA helicase activity GO:0003678 IEA
    damaged DNA binding GO:0003684 IEA
    double-stranded DNA binding GO:0003690 IBA
    double-stranded telomeric DNA binding GO:0003691 IDA
    RNA binding GO:0003723 HDA
    RNA binding GO:0003723 IDA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    enzyme activator activity GO:0008047 TAS
    ATP-dependent activity, acting on DNA GO:0008094 IDA
    ATP hydrolysis activity GO:0016887 IDA
    ubiquitin protein ligase binding GO:0031625 IPI
    U3 snoRNA binding GO:0034511 IDA
    telomeric DNA binding GO:0042162 IBA
    telomeric DNA binding GO:0042162 IDA
    telomeric DNA binding GO:0042162 TAS
    protein-containing complex binding GO:0044877 IPI
    DNA end binding GO:0045027 IDA
    DNA end binding GO:0045027 IDA
    5'-deoxyribose-5-phosphate lyase activity GO:0051575 IMP
Biological Process
    telomere maintenance GO:0000723 IBA
    recombinational repair GO:0000725 NAS
    activation of innate immune response GO:0002218 IDA
    double-strand break repair GO:0006302 IMP
    double-strand break repair via nonhomologous end joining GO:0006303 IBA
    double-strand break repair via nonhomologous end joining GO:0006303 IDA
    double-strand break repair via nonhomologous end joining GO:0006303 IMP
    double-strand break repair via nonhomologous end joining GO:0006303 NAS
    double-strand break repair via nonhomologous end joining GO:0006303 TAS
    DNA recombination GO:0006310 TAS
    DNA damage response GO:0006974 IMP
    telomere maintenance via telomerase GO:0007004 IMP
    response to xenobiotic stimulus GO:0009410 IEA
    neurogenesis GO:0022008 IEA
    regulation of telomere maintenance GO:0032204 TAS
    DNA duplex unwinding GO:0032508 IEA
    small-subunit processome assembly GO:0034462 IDA
    innate immune response GO:0045087 IEA
    positive regulation of protein kinase activity GO:0045860 IDA
    negative regulation of DNA-templated transcription GO:0045892 IMP
    regulation of smooth muscle cell proliferation GO:0048660 IMP
    positive regulation of neurogenesis GO:0050769 IEA
    hematopoietic stem cell differentiation GO:0060218 IEA
    protein localization to chromosome, telomeric region GO:0070198 IMP
    cellular response to fatty acid GO:0071398 IEA
    hematopoietic stem cell proliferation GO:0071425 IEA
    cellular hyperosmotic salinity response GO:0071475 IEA
    cellular response to gamma radiation GO:0071480 IDA
    cellular response to X-ray GO:0071481 IEA
    negative regulation of t-circle formation GO:1904430 IMP
    cellular response to leukemia inhibitory factor GO:1990830 IEA
Subcellular Localization
    chromosome, telomeric region GO:0000781 HDA
    chromosome, telomeric region GO:0000781 IDA
    nuclear telomere cap complex GO:0000783 TAS
    extracellular region GO:0005576 TAS
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    nucleolus GO:0005730 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
    DNA-dependent protein kinase-DNA ligase 4 complex GO:0005958 IDA
    membrane GO:0016020 HDA
    small-subunit processome GO:0032040 IDA
    protein-containing complex GO:0032991 IDA
    protein-DNA complex GO:0032993 IDA
    secretory granule lumen GO:0034774 TAS
    Ku70:Ku80 complex GO:0043564 IBA
    Ku70:Ku80 complex GO:0043564 IDA
    Ku70:Ku80 complex GO:0043564 IPI
    Ku70:Ku80 complex GO:0043564 TAS
    DNA-dependent protein kinase complex GO:0070418 IPI
    nonhomologous end joining complex GO:0070419 IDA
    site of DNA damage GO:0090734 IMP
    ribonucleoprotein complex GO:1990904 IDA
 Experiment description of studies that identified XRCC5 in sEVs
1
Experiment ID 76
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|GAPDH|HSP90|CD81|CD9|CD63|LAMP1|MHCI
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20224111    
Organism Homo sapiens
Experiment description Proteomics analysis of bladder cancer exosomes.
Authors "Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A."
Journal name MCP
Publication year 2010
Sample Bladder cancer cells
Sample name HT1376
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
FACS
2
Experiment ID 412
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MCF7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
3
Experiment ID 414
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
4
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 1334
MISEV standards
Biophysical techniques
✔
CD9
Enriched markers
✔
ACTB
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35918900    
Organism Homo sapiens
Experiment description Characterization of protein complexes in extracellular vesicles by intact extracellular vesicle crosslinking mass spectrometry (iEVXL)
Authors "Bauzá-Martinez J, Armony G, Pronker MF, Wu W. "
Journal name J Extracell Vesicles
Publication year 2022
Sample Breast cancer cells
Sample name LM2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectometry
7
Experiment ID 1336
MISEV standards
Biophysical techniques
✔
CD9
Enriched markers
✔
ACTB
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35918900    
Organism Homo sapiens
Experiment description Characterization of protein complexes in extracellular vesicles by intact extracellular vesicle crosslinking mass spectrometry (iEVXL)
Authors "Bauzá-Martinez J, Armony G, Pronker MF, Wu W. "
Journal name J Extracell Vesicles
Publication year 2022
Sample Breast cancer cells
Sample name LM2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectometry
8
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
10
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
11
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
12
Experiment ID 282
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
13
Experiment ID 283
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
14
Experiment ID 285
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Sequential centrifugal ultrafiltration - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Sequential centrifugal ultrafiltration
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
15
Experiment ID 286
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Sequential centrifugal ultrafiltration - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Sequential centrifugal ultrafiltration
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
16
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
17
Experiment ID 407
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|TSG101|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
18
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
19
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
20
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
21
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
22
Experiment ID 1275
MISEV standards
Biophysical techniques
✔
CD9|TSG101|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
Enriched markers
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
23
Experiment ID 1280
MISEV standards
Biophysical techniques
✔
TSG101|CD9|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
Enriched markers
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T - Fraction 5
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density 1.151 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
24
Experiment ID 405
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Foreskin fibroblasts
Sample name BJ
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
25
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
26
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
27
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
28
Experiment ID 417
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Lymphoma cells
Sample name Raji
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
29
Experiment ID 411
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Mammary cancer-associated fibroblasts
Sample name mCAF
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
30
Experiment ID 254
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name MNT-1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
31
Experiment ID 255
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name G1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
32
Experiment ID 256
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 501mel
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
33
Experiment ID 257
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name Daju
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
34
Experiment ID 260
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 1205Lu
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
35
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
36
Experiment ID 418
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Monocytic leukemia cells
Sample name THP-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
37
Experiment ID 418
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Monocytic leukemia cells
Sample name THP-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
38
Experiment ID 413
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Normal mammary epithelial cells
Sample name MCF10A
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
39
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
40
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
41
Experiment ID 406
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35|CD81
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name BxPC3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
42
Experiment ID 415
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
43
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
44
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
45
Experiment ID 408
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPDE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
46
Experiment ID 409
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPNE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
47
Experiment ID 416
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pluripotent stem cells
Sample name PSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
48
Experiment ID 145
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|CD10
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name C4-2 - Rep 3
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
49
Experiment ID 410
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample T lymphocytes
Sample name Jurkat
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
50
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
51
Experiment ID 193
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD9
Enriched markers
✔
PHB
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
52
Experiment ID 194
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
53
Experiment ID 195
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for XRCC5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Mdm2  
Affinity Capture-MS Mus musculus
2 HIST2H2BE 8349
Affinity Capture-MS Homo sapiens
3 ISG15 9636
Affinity Capture-MS Homo sapiens
4 MACROD1 28992
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
5 NFIB  
Affinity Capture-MS Homo sapiens
6 DYNC1I2 1781
Affinity Capture-MS Homo sapiens
7 NFATC1 4772
Affinity Capture-MS Homo sapiens
8 DDX17 10521
Affinity Capture-MS Homo sapiens
9 EBNA-LP  
Affinity Capture-MS
10 BRCA1 672
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
11 KIF20A 10112
Affinity Capture-MS Homo sapiens
12 CSNK2A1 1457
Biochemical Activity Homo sapiens
13 VHL  
Affinity Capture-MS Homo sapiens
14 SOX2  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 CENPA  
Affinity Capture-MS Homo sapiens
16 APEX1 328
Reconstituted Complex Homo sapiens
17 WDR76  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
18 DTX3  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
19 CHAF1A  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
20 KIF23 9493
Affinity Capture-MS Homo sapiens
21 MATR3 9782
Co-fractionation Homo sapiens
22 RNF8  
Affinity Capture-Western Homo sapiens
23 HDLBP 3069
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
24 TRDN 10345
Cross-Linking-MS (XL-MS) Homo sapiens
25 NCOA3  
Affinity Capture-MS Homo sapiens
26 ORC6  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
27 PPM1A 5494
Affinity Capture-MS Homo sapiens
28 MECP2 4204
Affinity Capture-MS Homo sapiens
29 RNF126  
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
30 SOD1 6647
Affinity Capture-MS Homo sapiens
31 RBM14 10432
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
32 NEIL3  
Affinity Capture-MS Homo sapiens
33 KIF14 9928
Affinity Capture-MS Homo sapiens
34 EMC9  
Affinity Capture-MS Homo sapiens
35 TFAP2A  
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
36 XRCC4  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
37 CHMP4C 92421
Affinity Capture-MS Homo sapiens
38 BRPF1  
Affinity Capture-MS Homo sapiens
39 GATA4  
Affinity Capture-MS Homo sapiens
40 VAV1 7409
Affinity Capture-Western Homo sapiens
41 SPTA1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
42 CRY1  
Affinity Capture-MS Homo sapiens
43 MRE11A 4361
Co-fractionation Homo sapiens
44 APLF  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
45 C17orf49 124944
Affinity Capture-MS Homo sapiens
46 HIST1H3E 8353
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
47 CDH1 999
Affinity Capture-MS Homo sapiens
48 TYK2 7297
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
49 TRIM21 6737
Affinity Capture-MS Homo sapiens
50 SAR1B 51128
Affinity Capture-MS Homo sapiens
51 NOS2  
Affinity Capture-MS Homo sapiens
52 RFC4 5984
Co-fractionation Homo sapiens
53 HECTD1 25831
Affinity Capture-MS Homo sapiens
54 CUL2 8453
Co-fractionation Homo sapiens
55 MPDZ  
Affinity Capture-MS Homo sapiens
56 BIN1 274
Co-purification Homo sapiens
57 LIMS1 3987
Affinity Capture-MS Homo sapiens
58 SCARNA22  
Affinity Capture-RNA Homo sapiens
59 ANLN 54443
Affinity Capture-MS Homo sapiens
60 SMYD5 10322
Affinity Capture-MS Homo sapiens
61 TMPO 7112
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 RBM39 9584
Affinity Capture-MS Homo sapiens
63 BKRF4  
Affinity Capture-MS
64 SIRT7  
Affinity Capture-MS Homo sapiens
65 EPAS1  
Affinity Capture-Western Homo sapiens
66 SMAD3 4088
Co-fractionation Homo sapiens
67 CUL3 8452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 Fbxl16  
Affinity Capture-MS Mus musculus
69 CSE1L 1434
Co-fractionation Homo sapiens
70 POLR2D  
Affinity Capture-Western Homo sapiens
71 EIF2S2 8894
Co-fractionation Homo sapiens
72 GZMA 3001
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
73 FN1 2335
Affinity Capture-MS Homo sapiens
74 THRA  
Affinity Capture-MS Homo sapiens
75 PCBP1 5093
Proximity Label-MS Homo sapiens
76 ERG  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
77 RPA3 6119
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
78 HOXC4  
Affinity Capture-Western Homo sapiens
79 HSPA1A 3303
Cross-Linking-MS (XL-MS) Homo sapiens
80 HSPA4 3308
Co-fractionation Homo sapiens
81 CSDE1 7812
Co-fractionation Homo sapiens
82 NFATC2  
Affinity Capture-MS Homo sapiens
83 SUPT16H 11198
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
84 PYHIN1  
Affinity Capture-MS Homo sapiens
85 PRKDC 5591
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
86 TLX1  
Affinity Capture-MS Homo sapiens
87 GRK5 2869
Affinity Capture-MS Homo sapiens
88 SNUPN 10073
Affinity Capture-MS Homo sapiens
89 RUVBL2 10856
Affinity Capture-MS Homo sapiens
90 ZEB1  
Affinity Capture-MS Homo sapiens
91 POLE3  
Affinity Capture-Western Homo sapiens
92 YARS 8565
Co-fractionation Homo sapiens
93 SSBP1 6742
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
94 DYNC1H1 1778
Co-fractionation Homo sapiens
95 NCL 4691
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
96 GOLGA5 9950
Affinity Capture-MS Homo sapiens
97 EIF4B 1975
Co-fractionation Homo sapiens
98 TRIM28 10155
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Co-fractionation Homo sapiens
99 POLA1  
Affinity Capture-Western Homo sapiens
100 CUL7 9820
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
101 ADRM1 11047
Co-fractionation Homo sapiens
102 ZZEF1 23140
Affinity Capture-MS Homo sapiens
103 ATR  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
104 LIG4 3981
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
105 ORC2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
106 EIF2S3 1968
Co-fractionation Homo sapiens
107 TARDBP 23435
Affinity Capture-MS Homo sapiens
108 RPA2 6118
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
109 HIST1H2BG 8339
Proximity Label-MS Homo sapiens
110 CD40 958
Reconstituted Complex Homo sapiens
111 ATM 472
Reconstituted Complex Homo sapiens
112 TERF2IP 54386
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
113 S100A14 57402
Affinity Capture-MS Homo sapiens
114 SAFB 6294
Co-fractionation Homo sapiens
115 NR1H4  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
116 B3GNT2 10678
Affinity Capture-MS Homo sapiens
117 DIAPH1 1729
Co-fractionation Homo sapiens
118 PRC1 9055
Affinity Capture-MS Homo sapiens
119 NPM1 4869
Co-fractionation Homo sapiens
120 PTER 9317
Co-fractionation Homo sapiens
121 OBSL1 23363
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
122 BAX 581
Affinity Capture-Western Homo sapiens
123 VCAM1 7412
Affinity Capture-MS Homo sapiens
124 PHF1  
Affinity Capture-Western Homo sapiens
125 SMARCAD1  
Affinity Capture-MS Homo sapiens
126 PLCB3 5331
Co-fractionation Homo sapiens
127 PRNP 5621
Affinity Capture-MS Homo sapiens
128 YWHAB 7529
Co-fractionation Homo sapiens
129 GZMB  
Biochemical Activity Homo sapiens
130 VRK3 51231
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
131 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
132 MSX2  
Affinity Capture-Western Homo sapiens
133 NBN 4683
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
134 CBL 867
Affinity Capture-MS Homo sapiens
135 VRK1 7443
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
136 AIM2  
Affinity Capture-MS Homo sapiens
137 RLIM 51132
Affinity Capture-MS Homo sapiens
138 PPM1G 5496
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
139 HNRNPD 3184
Affinity Capture-MS Homo sapiens
140 CHRAC1  
Affinity Capture-Western Homo sapiens
141 PSMA3 5684
Affinity Capture-MS Homo sapiens
142 NKX2-5  
Affinity Capture-MS Homo sapiens
143 KCND3 3752
Affinity Capture-Western Homo sapiens
144 TP53BP1 7158
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
145 XPO1 7514
Co-fractionation Homo sapiens
146 TPR 7175
Co-fractionation Homo sapiens
147 PPARG 5468
Affinity Capture-Western Homo sapiens
148 POLR2E 5434
Affinity Capture-Western Homo sapiens
149 EGFR 1956
Affinity Capture-MS Homo sapiens
Negative Genetic Homo sapiens
150 HSD17B10 3028
Affinity Capture-MS Homo sapiens
151 PCDH7 5099
Affinity Capture-MS Homo sapiens
152 CDK2 1017
Affinity Capture-MS Homo sapiens
153 PIP4K2A 5305
Co-fractionation Homo sapiens
154 MYC  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
155 BAG1 573
Affinity Capture-MS Homo sapiens
156 PDX1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
157 MIB2 142678
Affinity Capture-MS Homo sapiens
158 DDRGK1 65992
Affinity Capture-MS Homo sapiens
159 BMI1  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
160 NFYA 4800
Co-fractionation Homo sapiens
161 ADAR 103
Co-fractionation Homo sapiens
162 Rnf126  
Affinity Capture-Western Mus musculus
163 HUWE1 10075
Affinity Capture-MS Homo sapiens
164 RAD23A 5886
Affinity Capture-MS Homo sapiens
165 TERF2  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
166 PARP1 142
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
167 HDGF 3068
Affinity Capture-MS Homo sapiens
168 H3F3A 3020
Affinity Capture-MS Homo sapiens
169 MAX  
Co-fractionation Homo sapiens
170 SSRP1 6749
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
171 POU2F1 5451
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
172 ESR1  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
173 HEXIM1 10614
Co-fractionation Homo sapiens
174 BAG5 9529
Affinity Capture-MS Homo sapiens
175 EIF3J 8669
Co-fractionation Homo sapiens
176 IGF2R 3482
Affinity Capture-MS Homo sapiens
177 C1QBP 708
Co-fractionation Homo sapiens
178 KRAS 3845
Affinity Capture-MS Homo sapiens
Synthetic Lethality Homo sapiens
Negative Genetic Homo sapiens
179 MAST3  
Affinity Capture-MS Homo sapiens
180 DERL1 79139
Affinity Capture-MS Homo sapiens
181 PRMT1 3276
Affinity Capture-MS Homo sapiens
182 ZBTB7A 51341
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
183 HNRNPC 3183
Affinity Capture-Western Homo sapiens
184 SENP3 26168
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
185 ORC4  
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Co-localization Homo sapiens
186 THRB 7068
Affinity Capture-MS Homo sapiens
187 MME 4311
Affinity Capture-MS Homo sapiens
188 TCF7L2  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
189 NCOA6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
190 USP11 8237
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
191 PGR  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
192 RPS6KB2  
Affinity Capture-MS Homo sapiens
193 ATG13 9776
Affinity Capture-MS Homo sapiens
194 CDC16 8881
Two-hybrid Homo sapiens
195 MCM2 4171
Affinity Capture-MS Homo sapiens
196 MDC1  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
197 HMGA2 8091
Co-fractionation Homo sapiens
198 ITGA4 3676
Affinity Capture-MS Homo sapiens
199 ATRX 546
Affinity Capture-MS Homo sapiens
200 ATG16L1 55054
Affinity Capture-MS Homo sapiens
201 GOLGA4  
Co-fractionation Homo sapiens
202 RC3H2  
Affinity Capture-MS Homo sapiens
203 YAF2  
Affinity Capture-MS Homo sapiens
204 SAMHD1 25939
Co-fractionation Homo sapiens
205 NF2 4771
Affinity Capture-MS Homo sapiens
206 DLD 1738
Affinity Capture-MS Homo sapiens
207 UBE2T  
Affinity Capture-MS Homo sapiens
208 PARK2  
Affinity Capture-MS Homo sapiens
209 PNKP 11284
Affinity Capture-MS Homo sapiens
210 BTF3 689
Affinity Capture-MS Homo sapiens
211 BARD1 580
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
212 NTRK1 4914
Affinity Capture-MS Homo sapiens
213 CARM1 10498
Co-fractionation Homo sapiens
214 DHX9 1660
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
215 SLX4IP  
Affinity Capture-MS Homo sapiens
216 HDAC5 10014
Affinity Capture-MS Homo sapiens
217 DNM1L 10059
Affinity Capture-MS Homo sapiens
218 TONSL  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
219 CEP128  
Affinity Capture-MS Homo sapiens
220 METTL14  
Affinity Capture-MS Homo sapiens
221 MSH2 4436
Co-fractionation Homo sapiens
222 SMARCA5 8467
Affinity Capture-Western Homo sapiens
223 TBX20 57057
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
224 SF1 7536
Co-fractionation Homo sapiens
225 UHRF2  
Affinity Capture-MS Homo sapiens
226 YY1 7528
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
227 HNRNPA1 3178
Affinity Capture-MS Homo sapiens
228 COX6B1 1340
Affinity Capture-MS Homo sapiens
229 POLR2A 5430
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
230 RFC1 5981
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
231 RUNX2  
Affinity Capture-Western Homo sapiens
232 TOP1 7150
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
233 PCGF1 84759
Co-localization Homo sapiens
234 HYOU1 10525
Co-fractionation Homo sapiens
235 RNF2  
Affinity Capture-MS Homo sapiens
Co-localization Homo sapiens
236 EHF  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
237 ROCK1 6093
Affinity Capture-MS Homo sapiens
238 ILF3 3609
Co-fractionation Homo sapiens
239 XRCC5 7520
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
240 HIF1A 3091
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
241 TBX3 6926
Affinity Capture-MS Homo sapiens
242 AHSA1 10598
Affinity Capture-Western Homo sapiens
243 EIF2S1 1965
Co-fractionation Homo sapiens
244 PRMT3 10196
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
245 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
246 TP53 7157
Affinity Capture-MS Homo sapiens
247 HMGN4  
Affinity Capture-MS Homo sapiens
248 LIG3 3980
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
249 JUP 3728
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
250 RPA1 6117
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
251 IKBKG 8517
Affinity Capture-Western Homo sapiens
252 HSPB1 3315
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
253 CHD1L 9557
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
254 DNMBP 23268
Co-fractionation Homo sapiens
255 TRIM25 7706
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
256 PCNA 5111
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
257 PDZD8 118987
Affinity Capture-MS Homo sapiens
258 UFL1 23376
Affinity Capture-MS Homo sapiens
259 NFYB  
Co-fractionation Homo sapiens
260 ORC1  
Affinity Capture-Western Homo sapiens
261 SERBP1 26135
Affinity Capture-MS Homo sapiens
262 KDM2B 84678
Co-localization Homo sapiens
263 AR 367
Reconstituted Complex Homo sapiens
264 RC3H1 149041
Affinity Capture-MS Homo sapiens
265 ATXN3 4287
Affinity Capture-MS Homo sapiens
266 NUCKS1 64710
Affinity Capture-MS Homo sapiens
267 EP300 2033
Affinity Capture-MS Homo sapiens
268 UBE2H 7328
Affinity Capture-MS Homo sapiens
269 RIT1 6016
Negative Genetic Homo sapiens
270 FMNL1 752
Affinity Capture-MS Homo sapiens
271 COIL  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
272 MITD1 129531
Affinity Capture-MS Homo sapiens
273 MSH6 2956
Affinity Capture-Western Homo sapiens
274 WWP1 11059
Affinity Capture-MS Homo sapiens
275 PPIE 10450
Affinity Capture-MS Homo sapiens
276 USP5 8078
Co-fractionation Homo sapiens
277 NFYC 4802
Co-fractionation Homo sapiens
278 CDK5RAP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
279 KARS 3735
Co-fractionation Homo sapiens
280 SNRPD2 6633
Co-fractionation Homo sapiens
281 ILF2 3608
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
282 USP36  
Affinity Capture-MS Homo sapiens
283 KIAA1429 25962
Affinity Capture-MS Homo sapiens
284 TOP2A 7153
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
285 ERF  
Affinity Capture-MS Homo sapiens
286 MSH5  
Affinity Capture-MS Homo sapiens
287 KIF5B 3799
Co-fractionation Homo sapiens
288 RPL24 6152
Co-fractionation Homo sapiens
289 PRIM1  
Affinity Capture-Western Homo sapiens
290 HMGA1 3159
Affinity Capture-MS Homo sapiens
291 ABCE1 6059
Affinity Capture-MS Homo sapiens
292 PEAK1 79834
Affinity Capture-MS Homo sapiens
293 EED  
Affinity Capture-MS Homo sapiens
294 MAP3K6  
Affinity Capture-MS Homo sapiens
295 TERT  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
296 MYCN  
Affinity Capture-MS Homo sapiens
297 RAD50 10111
Co-fractionation Homo sapiens
298 NARS 4677
Co-fractionation Homo sapiens
299 LAMTOR1 55004
Affinity Capture-MS Homo sapiens
300 WRN 7486
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
301 H2AFZ 3015
Affinity Capture-MS Homo sapiens
302 GEMIN5 25929
Co-fractionation Homo sapiens
303 ECT2 1894
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
304 RNF146  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
305 BZLF1  
Affinity Capture-MS
306 UBE2I 7329
Biochemical Activity Homo sapiens
307 VCP 7415
Affinity Capture-MS Homo sapiens
308 IFI16 3428
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
309 CHEK1  
Affinity Capture-Western Homo sapiens
310 SUMO2 6613
Reconstituted Complex Homo sapiens
311 GANAB 23193
Co-fractionation Homo sapiens
312 H2AFY 9555
Affinity Capture-MS Homo sapiens
313 CEBPA  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
314 PRKACB 5567
Affinity Capture-MS Homo sapiens
315 ATP5B 506
Cross-Linking-MS (XL-MS) Homo sapiens
316 RTEL1 51750
Affinity Capture-MS Homo sapiens
317 TRMT1 55621
Co-fractionation Homo sapiens
318 HIST1H3A 8350
Proximity Label-MS Homo sapiens
319 FANCD2  
Affinity Capture-MS Homo sapiens
320 POLR2H 5437
Co-fractionation Homo sapiens
321 CTNNB1 1499
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
322 NAA15 80155
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
323 TFAP4 7023
Co-fractionation Homo sapiens
324 ASAP3 55616
Affinity Capture-MS Homo sapiens
325 HOXB7 3217
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
326 SUZ12  
Affinity Capture-MS Homo sapiens
327 AURKB 9212
Affinity Capture-MS Homo sapiens
328 NDST1 3340
Affinity Capture-MS Homo sapiens
329 DNAJC9 23234
Proximity Label-MS Homo sapiens
330 BAZ1A 11177
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
331 VDAC1 7416
Affinity Capture-MS Homo sapiens
332 NANOG  
Affinity Capture-MS Homo sapiens
333 CCDC8  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
334 H2AFX 3014
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
335 TXLNA 200081
Co-fractionation Homo sapiens
336 ARRB2 409
Affinity Capture-MS Homo sapiens
337 CLIC1 1192
Co-fractionation Homo sapiens
338 SIRT6  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
339 KAT2A  
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
340 MINA 84864
Co-fractionation Homo sapiens
341 ZNF746  
Affinity Capture-MS Homo sapiens
342 PHRF1  
Affinity Capture-Western Homo sapiens
343 ORC3  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
344 DGCR8  
Affinity Capture-MS Homo sapiens
345 HMGN5 79366
Affinity Capture-MS Homo sapiens
346 SMURF1 57154
Affinity Capture-MS Homo sapiens
347 VTA1 51534
Co-fractionation Homo sapiens
348 ELF3 1999
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
349 TERF1 7013
Affinity Capture-MS Homo sapiens
350 ARRB1 408
Affinity Capture-MS Homo sapiens
351 HSF1  
Reconstituted Complex Homo sapiens
352 UPF1 5976
Co-fractionation Homo sapiens
353 XRCC6 2547
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Proximity Label-MS Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
354 C9orf72  
Affinity Capture-MS Homo sapiens
355 USF1  
Affinity Capture-Western Homo sapiens
356 PAF1 54623
Co-fractionation Homo sapiens
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Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here