Gene description for RAD23A
Gene name RAD23 homolog A (S. cerevisiae)
Gene symbol RAD23A
Other names/aliases HHR23A
HR23A
Species Homo sapiens
 Database cross references - RAD23A
ExoCarta ExoCarta_5886
Vesiclepedia VP_5886
Entrez Gene 5886
HGNC 9812
MIM 600061
UniProt P54725  
 RAD23A identified in exosomes derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
 Gene ontology annotations for RAD23A
Molecular Function
    ubiquitin-specific protease binding GO:1990381 IPI
    protein binding GO:0005515 IPI
    polyubiquitin binding GO:0031593 IDA
    damaged DNA binding GO:0003684 IEA
    single-stranded DNA binding GO:0003697 TAS
Biological Process
    nucleotide-excision repair GO:0006289 IDA
    positive regulation of viral genome replication GO:0045070 IMP
    viral process GO:0016032 IEA
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IEA
    regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032434 IDA
Subcellular Localization
    proteasome complex GO:0000502 IEA
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified RAD23A in exosomes
1
Experiment ID 363
MISEV standards
EV Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
EV Enriched markers
DCLK1
EV Negative markers
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
EV Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
EV Enriched markers
DCLK1
EV Negative markers
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
EV Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
EV Enriched markers
DCLK1
EV Negative markers
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for RAD23A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PDE6D 5147
Two-hybrid Homo sapiens
2 TRAF2 7186
Two-hybrid Homo sapiens
3 UBL7  
Two-hybrid Homo sapiens
4 MPG 4350
Affinity Capture-MS Homo sapiens
5 RPN1 6184
Affinity Capture-MS Homo sapiens
6 ATXN3 4287
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
7 EWSR1 2130
Two-hybrid Homo sapiens
8 SQSTM1 8878
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
9 PSMD4 5710
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
10 UBI4  
Co-crystal Structure 4932
11 XPC  
Invivo Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which RAD23A is involved
No pathways found





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