Gene description for RAD23A
Gene name
RAD23 homolog A (S. cerevisiae)
Gene symbol
RAD23A
Other names/aliases
HHR23A HR23A
Species
Homo sapiens
Database cross references - RAD23A
ExoCarta
ExoCarta_5886
Vesiclepedia
VP_5886
Entrez Gene
5886
HGNC
9812
MIM
600061
UniProt
P54725
RAD23A identified in exosomes derived from the following tissue/cell type
Gastric cancer cells
33991177
Gastric cancer cells
33991177
Gastric cancer cells
33991177
Gene ontology annotations for RAD23A
Experiment description of studies that identified RAD23A in exosomes
1
Experiment ID
363
MISEV standards
✘
EV Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
EV Enriched markers
✔
DCLK1
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
33991177
Organism
Homo sapiens
Experiment description
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors
Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name
Proteomics
Publication year
2021
Sample
Gastric cancer cells
Sample name
MKN1 - 100K pellet
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
2
Experiment ID
364
MISEV standards
✘
EV Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
EV Enriched markers
✔
DCLK1
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
33991177
Organism
Homo sapiens
Experiment description
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors
Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name
Proteomics
Publication year
2021
Sample
Gastric cancer cells
Sample name
MKN1 - 100K pellet
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
3
Experiment ID
365
MISEV standards
✘
EV Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
EV Enriched markers
✔
DCLK1
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
33991177
Organism
Homo sapiens
Experiment description
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors
Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name
Proteomics
Publication year
2021
Sample
Gastric cancer cells
Sample name
MKN1 - 100K pellet
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
Protein-protein interactions for RAD23A
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
PDE6D
5147
Two-hybrid
Homo sapiens
2
TRAF2
7186
Two-hybrid
Homo sapiens
3
UBL7
Two-hybrid
Homo sapiens
4
MPG
4350
Affinity Capture-MS
Homo sapiens
5
RPN1
6184
Affinity Capture-MS
Homo sapiens
6
ATXN3
4287
Invivo
Homo sapiens
Affinity Capture-MS
Homo sapiens
7
EWSR1
2130
Two-hybrid
Homo sapiens
8
SQSTM1
8878
Two-hybrid
Homo sapiens
Affinity Capture-Western
Homo sapiens
9
PSMD4
5710
Affinity Capture-MS
Homo sapiens
Two-hybrid
Homo sapiens
Reconstituted Complex
Homo sapiens
10
UBI4
Co-crystal Structure
4932
11
XPC
Invivo
Homo sapiens
Two-hybrid
Homo sapiens
View the network
image/svg+xml
Pathways in which RAD23A is involved
No pathways found