Gene description for NGLY1
Gene name N-glycanase 1
Gene symbol NGLY1
Other names/aliases CDG1V
PNG1
PNGase
Species Homo sapiens
 Database cross references - NGLY1
ExoCarta ExoCarta_55768
Vesiclepedia VP_55768
Entrez Gene 55768
HGNC 17646
MIM 610661
UniProt Q96IV0  
 NGLY1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Squamous carcinoma cells 20124223    
Thymus 23844026    
 Gene ontology annotations for NGLY1
Molecular Function
    peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase activity GO:0000224 IBA
    peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase activity GO:0000224 IGI
    peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase activity GO:0000224 TAS
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
Biological Process
    protein folding GO:0006457 TAS
    glycoprotein catabolic process GO:0006516 IBA
    glycoprotein catabolic process GO:0006516 IDA
    positive regulation of BMP signaling pathway GO:0030513 IBA
Subcellular Localization
    nucleus GO:0005634 IBA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified NGLY1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for NGLY1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NPAS2  
Two-hybrid Homo sapiens
2 UBXN2B  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
3 RAD23A 5886
Two-hybrid Homo sapiens
Co-crystal Structure Homo sapiens
Two-hybrid Homo sapiens
4 PRTN3 5657
Affinity Capture-MS Homo sapiens
5 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
6 GUCD1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
7 CEACAM8 1088
Affinity Capture-MS Homo sapiens
8 OIT3 170392
Affinity Capture-MS Homo sapiens
9 PSG1  
Affinity Capture-MS Homo sapiens
10 GLTSCR1L  
Two-hybrid Homo sapiens
11 NSFL1C 55968
Reconstituted Complex Homo sapiens
12 TP53BP1 7158
Negative Genetic Homo sapiens
13 SRPK2 6733
Biochemical Activity Homo sapiens
14 SGSH 6448
Affinity Capture-MS Homo sapiens
15 TMEM25  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 EFNA2  
Affinity Capture-MS Homo sapiens
17 ASIC4  
Affinity Capture-MS Homo sapiens
18 IFI30  
Affinity Capture-MS Homo sapiens
19 RAD23B 5887
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
20 VMAC  
Two-hybrid Homo sapiens
21 B3GNT3 10331
Affinity Capture-MS Homo sapiens
22 EGFR 1956
Affinity Capture-MS Homo sapiens
23 TRAFD1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
24 BOC  
Affinity Capture-MS Homo sapiens
25 FBXO6 26270
Affinity Capture-MS Homo sapiens
26 LINGO1 84894
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 NAGPA 51172
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 APP 351
Reconstituted Complex Homo sapiens
29 TRIM54  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
30 NPTX1  
Affinity Capture-MS Homo sapiens
31 AMIGO1  
Affinity Capture-MS Homo sapiens
32 PAX5  
Two-hybrid Homo sapiens
33 CER1  
Affinity Capture-MS Homo sapiens
34 TSHR 7253
Affinity Capture-MS Homo sapiens
35 B4GALT4 8702
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 CD79A  
Affinity Capture-MS Homo sapiens
37 ACP5  
Affinity Capture-MS Homo sapiens
38 CRLF1 9244
Affinity Capture-MS Homo sapiens
39 VCP 7415
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
40 FAF1 11124
Reconstituted Complex Homo sapiens
41 TCF12  
Two-hybrid Homo sapiens
42 GXYLT1 283464
Affinity Capture-MS Homo sapiens
43 MEN1 4221
Affinity Capture-MS Homo sapiens
44 PDCD1  
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
45 CST9L  
Affinity Capture-MS Homo sapiens
46 ATXN3 4287
Affinity Capture-MS Homo sapiens
47 LIPF  
Affinity Capture-MS Homo sapiens
48 RTN4R 65078
Affinity Capture-MS Homo sapiens
49 UBQLN1 29979
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
50 PTPRO 5800
Affinity Capture-MS Homo sapiens
51 GOLGB1 2804
Cross-Linking-MS (XL-MS) Homo sapiens
52 ACBD5 91452
Proximity Label-MS Homo sapiens
53 DERL1 79139
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
54 ICAM4  
Affinity Capture-MS Homo sapiens
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