Gene description for NGLY1
Gene name N-glycanase 1
Gene symbol NGLY1
Other names/aliases CDG1V
PNG1
PNGase
Species Homo sapiens
 Database cross references - NGLY1
ExoCarta ExoCarta_55768
Entrez Gene 55768
HGNC 17646
MIM 610661
UniProt Q96IV0  
 NGLY1 identified in exosomes derived from the following tissue/cell type
Squamous carcinoma cells 20124223    
Thymus 23844026    
 Gene ontology annotations for NGLY1
Molecular Function
    metal ion binding GO:0046872 IEA
    peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase activity GO:0000224 IEA
    protein binding GO:0005515 IPI
Biological Process
    glycoprotein catabolic process GO:0006516 IDA
Subcellular Localization
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified NGLY1 in exosomes
1
Experiment ID 191
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD81|CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for NGLY1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which NGLY1 is involved
No pathways found





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