Gene description for SGSH
Gene name N-sulfoglucosamine sulfohydrolase
Gene symbol SGSH
Other names/aliases HSS
MPS3A
SFMD
Species Homo sapiens
 Database cross references - SGSH
ExoCarta ExoCarta_6448
Entrez Gene 6448
HGNC 10818
MIM 605270
UniProt P51688  
 SGSH identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
 Gene ontology annotations for SGSH
Molecular Function
    metal ion binding GO:0046872 IEA
    sulfuric ester hydrolase activity GO:0008484 IEA
    catalytic activity GO:0003824 TAS
    N-sulfoglucosamine sulfohydrolase activity GO:0016250 IEA
Biological Process
    glycosaminoglycan catabolic process GO:0006027 TAS
    carbohydrate metabolic process GO:0005975 TAS
    glycosaminoglycan metabolic process GO:0030203 TAS
    small molecule metabolic process GO:0044281 TAS
    proteoglycan metabolic process GO:0006029 TAS
Subcellular Localization
    extracellular exosome GO:0070062 IDA
    lysosomal lumen GO:0043202 TAS
 Experiment description of studies that identified SGSH in exosomes
1
Experiment ID 226
ISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH
EV Cytosolic markers
✔
CD9|FLOT1
EV Membrane markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
✘ EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
 Protein-protein interactions for SGSH
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which SGSH is involved
PathwayEvidenceSource
HS-GAG degradation TAS Reactome
MPS IIIA - Sanfilippo syndrome A TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here