Gene description for RRM1
Gene name ribonucleotide reductase M1
Gene symbol RRM1
Other names/aliases R1
RIR1
RR1
Species Homo sapiens
 Database cross references - RRM1
ExoCarta ExoCarta_6240
Entrez Gene 6240
HGNC 10451
MIM 180410
UniProt P23921  
 RRM1 identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for RRM1
Molecular Function
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor GO:0004748 ISS
Biological Process
    protein heterotetramerization GO:0051290 IEA
    retina development in camera-type eye GO:0060041 IEA
    nucleobase-containing small molecule interconversion GO:0015949 TAS
    deoxyribonucleotide biosynthetic process GO:0009263 ISS
    DNA replication GO:0006260 IEA
    oxidation-reduction process GO:0055114 IEA
    pyrimidine nucleobase metabolic process GO:0006206 IEA
    male gonad development GO:0008584 IEA
    mitotic cell cycle GO:0000278 IEA
    nucleobase-containing small molecule metabolic process GO:0055086 TAS
    cell proliferation in forebrain GO:0021846 IEA
    small molecule metabolic process GO:0044281 TAS
    response to ionizing radiation GO:0010212 IEA
Subcellular Localization
    nuclear envelope GO:0005635 IEA
    cytosol GO:0005829 TAS
    cytoplasm GO:0005737 IDA
    extracellular exosome GO:0070062 IDA
    neuronal cell body GO:0043025 IEA
    cell projection GO:0042995 IEA
    nucleoplasm GO:0005654 TAS
 Experiment description of studies that identified RRM1 in exosomes
1
Experiment ID 224
ISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✔
GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RRM1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RRM2B  
Affinity Capture-Western Homo sapiens
2 RRM2 6241
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
3 RRM1 6240
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
4 RIF1  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which RRM1 is involved
PathwayEvidenceSource
Synthesis and interconversion of nucleotide di- and triphosphates TAS Reactome





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