Gene description for LCMT1
Gene name leucine carboxyl methyltransferase 1
Gene symbol LCMT1
Other names/aliases CGI-68
LCMT
PPMT1
Species Homo sapiens
 Database cross references - LCMT1
ExoCarta ExoCarta_51451
Vesiclepedia VP_51451
Entrez Gene 51451
HGNC 17557
MIM 610286
UniProt Q9UIC8  
 LCMT1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for LCMT1
Molecular Function
    protein C-terminal carboxyl O-methyltransferase activity GO:0003880 IDA
    protein binding GO:0005515 IPI
    S-adenosylmethionine-dependent methyltransferase activity GO:0008757 TAS
    protein C-terminal leucine carboxyl O-methyltransferase activity GO:0018423 IBA
    protein C-terminal leucine carboxyl O-methyltransferase activity GO:0018423 TAS
Biological Process
    G2/M transition of mitotic cell cycle GO:0000086 TAS
    protein methylation GO:0006479 IMP
    C-terminal protein methylation GO:0006481 IDA
    regulation of glucose metabolic process GO:0010906 IEA
    negative regulation of protein-containing complex assembly GO:0031333 IMP
    protein modification process GO:0036211 TAS
    regulation of apoptotic process GO:0042981 IMP
    regulation of mitotic cell cycle spindle assembly checkpoint GO:0090266 IBA
    regulation of mitotic cell cycle spindle assembly checkpoint GO:0090266 IMP
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    cytosol GO:0005829 IBA
 Experiment description of studies that identified LCMT1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for LCMT1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PPP4C 5531
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 PPP4R2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 TANGO2  
Co-fractionation Homo sapiens
4 ADSL 158
Co-fractionation Homo sapiens
5 EIF1AX 1964
Affinity Capture-MS Homo sapiens
6 VPS37C 55048
Affinity Capture-MS Homo sapiens
7 BRCA1 672
Two-hybrid Homo sapiens
8 CUL3 8452
Affinity Capture-MS Homo sapiens
9 TAB1 10454
Two-hybrid Homo sapiens
10 NECAP1 25977
Co-fractionation Homo sapiens
11 FASN 2194
Negative Genetic Homo sapiens
12 DND1  
Affinity Capture-MS Homo sapiens
13 SERBP1 26135
Affinity Capture-MS Homo sapiens
14 FXR2 9513
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
15 ME1 4199
Co-fractionation Homo sapiens
16 RRM1 6240
Co-fractionation Homo sapiens
17 CASP3 836
Co-fractionation Homo sapiens
18 PPP2CA 5515
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 LDLR 3949
Negative Genetic Homo sapiens
20 RPA3 6119
Proximity Label-MS Homo sapiens
21 TGDS  
Affinity Capture-MS Homo sapiens
22 CMPK1 51727
Co-fractionation Homo sapiens
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