Gene description for NECAP1
Gene name NECAP endocytosis associated 1
Gene symbol NECAP1
Other names/aliases EIEE21
Species Homo sapiens
 Database cross references - NECAP1
ExoCarta ExoCarta_25977
Vesiclepedia VP_25977
Entrez Gene 25977
HGNC 24539
MIM 611623
UniProt Q8NC96  
 NECAP1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for NECAP1
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    endocytosis GO:0006897 IEA
    protein transport GO:0015031 IEA
    vesicle-mediated transport GO:0016192 IBA
Subcellular Localization
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IEA
    clathrin-coated pit GO:0005905 IEA
    clathrin vesicle coat GO:0030125 IBA
 Experiment description of studies that identified NECAP1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for NECAP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CACTIN  
Affinity Capture-MS Homo sapiens
2 AP2S1 1175
Affinity Capture-MS Homo sapiens
3 GTSE1 51512
Affinity Capture-MS Homo sapiens
4 NUMA1 4926
Affinity Capture-MS Homo sapiens
5 RPS27A 6233
Affinity Capture-MS Homo sapiens
6 AP2B1 163
Affinity Capture-MS Homo sapiens
7 KIAA0907  
Co-fractionation Homo sapiens
8 AP1M1 8907
Affinity Capture-MS Homo sapiens
9 PARP1 142
Affinity Capture-MS Homo sapiens
10 UFC1 51506
Co-fractionation Homo sapiens
11 APOBEC3C 27350
Affinity Capture-MS Homo sapiens
12 BAZ1B 9031
Affinity Capture-MS Homo sapiens
13 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
14 HIST3H2BB 128312
Affinity Capture-MS Homo sapiens
15 SMARCA5 8467
Affinity Capture-MS Homo sapiens
16 HIST1H1D 3007
Affinity Capture-MS Homo sapiens
17 H2AFZ 3015
Affinity Capture-MS Homo sapiens
18 REL 5966
Two-hybrid Homo sapiens
19 ARHGAP21 57584
Affinity Capture-MS Homo sapiens
20 PHIP 55023
Affinity Capture-MS Homo sapiens
21 RIF1  
Affinity Capture-MS Homo sapiens
22 AP2A1 160
Affinity Capture-MS Homo sapiens
23 EPS15 2060
Affinity Capture-MS Homo sapiens
24 SMARCA1 6594
Affinity Capture-MS Homo sapiens
25 MRE11A 4361
Affinity Capture-MS Homo sapiens
26 TOP2B 7155
Affinity Capture-MS Homo sapiens
27 HIST1H2BD 3017
Affinity Capture-MS Homo sapiens
28 H1F0 3005
Affinity Capture-MS Homo sapiens
29 WHSC1 7468
Affinity Capture-MS Homo sapiens
30 LCMT1 51451
Co-fractionation Homo sapiens
31 SPATA1  
Affinity Capture-MS Homo sapiens
32 SUZ12  
Affinity Capture-MS Homo sapiens
33 APP 351
Reconstituted Complex Homo sapiens
34 RAD50 10111
Affinity Capture-MS Homo sapiens
35 AP2M1 1173
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 PITPNA 5306
Co-fractionation Homo sapiens
37 KAT7  
Affinity Capture-MS Homo sapiens
38 CBX3 11335
Affinity Capture-MS Homo sapiens
39 TOP2A 7153
Affinity Capture-MS Homo sapiens
40 NECAP2 55707
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 NCL 4691
Cross-Linking-MS (XL-MS) Homo sapiens
42 MCM4 4173
Affinity Capture-MS Homo sapiens
43 HIST2H2AA3 8337
Affinity Capture-MS Homo sapiens
44 KIF22  
Affinity Capture-MS Homo sapiens
45 H1FX 8971
Affinity Capture-MS Homo sapiens
46 AP2A2 161
Affinity Capture-MS Homo sapiens
47 EPB41 2035
Affinity Capture-MS Homo sapiens
48 AP1M2 10053
Affinity Capture-MS Homo sapiens
49 HIST2H3A 333932
Affinity Capture-MS Homo sapiens
50 HIST1H1C 3006
Affinity Capture-MS Homo sapiens
51 AP1G1 164
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
52 MDC1  
Affinity Capture-MS Homo sapiens
53 CARHSP1 23589
Co-fractionation Homo sapiens
54 CLTC 1213
Affinity Capture-MS Homo sapiens
55 H2AFY2 55506
Affinity Capture-MS Homo sapiens
56 RAB4B 53916
Affinity Capture-MS Homo sapiens
57 MPI 4351
Co-fractionation Homo sapiens
58 H2AFY 9555
Affinity Capture-MS Homo sapiens
59 HIST1H2AJ 8331
Affinity Capture-MS Homo sapiens
60 TMUB1  
Affinity Capture-MS Homo sapiens
61 PHGDH 26227
Co-fractionation Homo sapiens
View the network image/svg+xml



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