Gene description for MCM4
Gene name minichromosome maintenance complex component 4
Gene symbol MCM4
Other names/aliases CDC21
CDC54
NKCD
NKGCD
P1-CDC21
hCdc21
Species Homo sapiens
 Database cross references - MCM4
ExoCarta ExoCarta_4173
Entrez Gene 4173
HGNC 6947
MIM 602638
UniProt P33991  
 MCM4 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Squamous carcinoma cells 20124223    
Thymus 23844026    
 Gene ontology annotations for MCM4
Molecular Function
    single-stranded DNA binding GO:0003697 IEA
    ATP binding GO:0005524 IEA
    protein binding GO:0005515 IPI
    ATP-dependent DNA helicase activity GO:0004003 IDA
Biological Process
    mitotic cell cycle GO:0000278 TAS
    DNA strand elongation involved in DNA replication GO:0006271 TAS
    DNA replication initiation GO:0006270 IEA
    DNA replication GO:0006260 TAS
    DNA unwinding involved in DNA replication GO:0006268 IEA
    G1/S transition of mitotic cell cycle GO:0000082 TAS
Subcellular Localization
    membrane GO:0016020 IDA
    nucleus GO:0005634 NAS
    MCM complex GO:0042555 IDA
    nucleoplasm GO:0005654 TAS
 Experiment description of studies that identified MCM4 in exosomes
1
Experiment ID 236
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 191
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD81|CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MCM4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RPA2 6118
Two-hybrid Homo sapiens
2 ASK  
Two-hybrid Homo sapiens
3 MCM7 4176
Invivo Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Reconstituted Complex Homo sapiens
4 RPA1 6117
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
5 MCM8 84515
Affinity Capture-Western Homo sapiens
6 ORC1L  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
7 CDC7  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
8 MCM6 4175
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
9 MCM4 4173
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
10 ORC6L  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
11 ORC5L  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
12 ORC2L  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
13 ORC3L  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
14 MCM2 4171
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
15 MCM10 55388
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
16 ORC4L  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
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