Gene description for MCM8
Gene name minichromosome maintenance complex component 8
Gene symbol MCM8
Other names/aliases C20orf154
POF10
dJ967N21.5
Species Homo sapiens
 Database cross references - MCM8
ExoCarta ExoCarta_84515
Entrez Gene 84515
HGNC 16147
MIM 608187
UniProt Q9UJA3  
 MCM8 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for MCM8
Molecular Function
    helicase activity GO:0004386 IEA
    DNA binding GO:0003677 IEA
    ATP binding GO:0005524 IEA
    protein binding GO:0005515 IPI
Biological Process
    mitotic cell cycle GO:0000278 TAS
    DNA strand elongation involved in DNA replication GO:0006271 TAS
    double-strand break repair via homologous recombination GO:0000724 IEA
    male gamete generation GO:0048232 ISS
    DNA replication GO:0006260 TAS
    female gamete generation GO:0007292 ISS
    cellular response to DNA damage stimulus GO:0006974 IDA
    G1/S transition of mitotic cell cycle GO:0000082 TAS
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    MCM8-MCM9 complex GO:0097362 IDA
    nucleus GO:0005634 IDA
 Experiment description of studies that identified MCM8 in exosomes
1
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for MCM8
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MCM4 4173
Affinity Capture-Western Homo sapiens
2 MCM6 4175
Affinity Capture-Western Homo sapiens
3 MCM7 4176
Affinity Capture-Western Homo sapiens
View the network image/svg+xml



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