Gene description for MCM8
Gene name minichromosome maintenance complex component 8
Gene symbol MCM8
Other names/aliases C20orf154
POF10
dJ967N21.5
Species Homo sapiens
 Database cross references - MCM8
ExoCarta ExoCarta_84515
Vesiclepedia VP_84515
Entrez Gene 84515
HGNC 16147
MIM 608187
UniProt Q9UJA3  
 MCM8 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for MCM8
Molecular Function
    chromatin binding GO:0003682 IDA
    DNA clamp loader activity GO:0003689 IEA
    single-stranded DNA binding GO:0003697 IBA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    four-way junction helicase activity GO:0009378 IEA
    ATP hydrolysis activity GO:0016887 IEA
    single-stranded DNA helicase activity GO:0017116 IBA
    enzyme binding GO:0019899 IPI
    MutLbeta complex binding GO:0032406 IDA
    MutSalpha complex binding GO:0032407 IDA
    MutSbeta complex binding GO:0032408 IDA
    double-stranded DNA helicase activity GO:0036121 IEA
    forked DNA-dependent helicase activity GO:0061749 IEA
    cohesin loader activity GO:0061775 IEA
    chromatin extrusion motor activity GO:0140584 IEA
    ATP-dependent H3-H4 histone complex chaperone activity GO:0140665 IEA
    ATP-dependent H2AZ histone chaperone activity GO:0140849 IEA
    single-stranded 3'-5' DNA helicase activity GO:1990518 IEA
Biological Process
    double-strand break repair via homologous recombination GO:0000724 IBA
    double-strand break repair via homologous recombination GO:0000724 IMP
    double-strand break repair via homologous recombination GO:0000724 NAS
    DNA replication GO:0006260 IGI
    chromatin remodeling GO:0006338 IEA
    DNA damage response GO:0006974 IDA
    female gamete generation GO:0007292 ISS
    DNA duplex unwinding GO:0032508 IDA
    recombinational interstrand cross-link repair GO:0036298 IMP
    male gamete generation GO:0048232 ISS
    protein stabilization GO:0050821 IMP
    mismatch repair involved in maintenance of fidelity involved in DNA-dependent DNA replication GO:0070716 NAS
    protein localization to chromatin GO:0071168 IMP
    chromatin looping GO:0140588 IEA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
    chromosome GO:0005694 IEA
    MCM complex GO:0042555 IBA
    MCM8-MCM9 complex GO:0097362 IDA
    MCM8-MCM9 complex GO:0097362 IPI
 Experiment description of studies that identified MCM8 in exosomes
1
Experiment ID 237
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for MCM8
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MCM7 4176
Affinity Capture-Western Homo sapiens
2 IL17RA 23765
Affinity Capture-MS Homo sapiens
3 MCM8 84515
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
4 FAM167A  
Affinity Capture-MS Homo sapiens
5 FASN 2194
Negative Genetic Homo sapiens
6 GAGE1  
Affinity Capture-MS Homo sapiens
7 TUBB 203068
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 SMARCA2 6595
Affinity Capture-MS Homo sapiens
9 AVIL  
Affinity Capture-MS Homo sapiens
10 TP53 7157
Synthetic Growth Defect Homo sapiens
11 MSH2 4436
Affinity Capture-MS Homo sapiens
12 KLK5 25818
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 XPO1 7514
Affinity Capture-MS Homo sapiens
14 LMNB1 4001
Affinity Capture-MS Homo sapiens
15 TSPYL6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 LOC100128554  
Affinity Capture-MS Homo sapiens
17 MCM9 254394
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
18 MCM5 4174
Affinity Capture-MS Homo sapiens
19 P4HA2 8974
Affinity Capture-MS Homo sapiens
20 MRPS25 64432
Affinity Capture-MS Homo sapiens
21 FEM1C  
Two-hybrid Homo sapiens
22 MIF 4282
Affinity Capture-MS Homo sapiens
23 SKP2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 AIM1L  
Affinity Capture-MS Homo sapiens
25 ORC2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
26 CDC6  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
27 BPNT1 10380
Affinity Capture-MS Homo sapiens
28 TGM2 7052
Affinity Capture-MS Homo sapiens
29 MCM4 4173
Affinity Capture-Western Homo sapiens
30 VWA5A 4013
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 IQCF1  
Affinity Capture-MS Homo sapiens
32 SNX21  
Affinity Capture-MS Homo sapiens
33 KLHL40  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 MLH1 4292
Affinity Capture-MS Homo sapiens
35 MSH6 2956
Affinity Capture-MS Homo sapiens
36 LRRC27  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 SKAP1 8631
Affinity Capture-MS Homo sapiens
38 FKBP5 2289
Affinity Capture-MS Homo sapiens
39 SREBF2 6721
Positive Genetic Homo sapiens
40 HSD17B7P2  
Affinity Capture-MS Homo sapiens
41 MCMBP 79892
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
42 COPB2 9276
Affinity Capture-MS Homo sapiens
43 LDLR 3949
Negative Genetic Homo sapiens
44 PTGES3 10728
Affinity Capture-MS Homo sapiens
45 F9 2158
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 MCM6 4175
Affinity Capture-Western Homo sapiens
47 DEF8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 HORMAD2  
Affinity Capture-MS Homo sapiens
49 C17orf53  
Proximity Label-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
50 PSG8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 CAPN2 824
Affinity Capture-MS Homo sapiens
52 RAP2A 5911
Affinity Capture-MS Homo sapiens
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