Gene description for MCM6
Gene name minichromosome maintenance complex component 6
Gene symbol MCM6
Other names/aliases MCG40308
Mis5
P105MCM
Species Homo sapiens
 Database cross references - MCM6
ExoCarta ExoCarta_4175
Entrez Gene 4175
HGNC 6949
MIM 601806
UniProt Q14566  
 MCM6 identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for MCM6
Molecular Function
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 NAS
    single-stranded DNA binding GO:0003697 IEA
    ATP-dependent DNA helicase activity GO:0004003 IDA
    identical protein binding GO:0042802 IPI
Biological Process
    G1/S transition of mitotic cell cycle GO:0000082 TAS
    DNA unwinding involved in DNA replication GO:0006268 IEA
    DNA replication initiation GO:0006270 IEA
    mitotic cell cycle GO:0000278 TAS
    DNA replication GO:0006260 TAS
    DNA strand elongation involved in DNA replication GO:0006271 TAS
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    nucleus GO:0005634 NAS
    MCM complex GO:0042555 IDA
 Experiment description of studies that identified MCM6 in exosomes
1
Experiment ID 226
ISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH
EV Cytosolic markers
✔
CD9|FLOT1
EV Membrane markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
✘ EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
2
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MCM6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SCNM1  
Two-hybrid Homo sapiens
2 PSMA1 5682
Two-hybrid Homo sapiens
3 MCM10 55388
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
4 IL1F5  
Two-hybrid Homo sapiens
5 MCM7 4176
Invivo Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
6 MCM3 4172
Affinity Capture-MS Homo sapiens
7 RPA1 6117
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
8 MCM8 84515
Affinity Capture-Western Homo sapiens
9 GMNN  
Affinity Capture-MS Homo sapiens
10 ORC1L  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
11 MLLT3 4300
Two-hybrid Homo sapiens
12 CDC45L  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
13 MCM6 4175
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
14 MCM4 4173
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
15 ORC2L  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
16 CCNA1  
Affinity Capture-MS Homo sapiens
17 MCM2 4171
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
18 ALS2CR11  
Two-hybrid Homo sapiens
19 ING5  
Two-hybrid Homo sapiens
20 ORC4L  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
21 CDT1  
Invitro Homo sapiens
Invivo Homo sapiens
Two-hybrid Homo sapiens
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