Gene description for USP49
Gene name ubiquitin specific peptidase 49
Gene symbol USP49
Other names/aliases -
Species Homo sapiens
 Database cross references - USP49
ExoCarta ExoCarta_25862
Vesiclepedia VP_25862
Entrez Gene 25862
HGNC 20078
UniProt Q70CQ1  
 USP49 identified in exosomes derived from the following tissue/cell type
Thymus 23844026    
 Gene ontology annotations for USP49
Molecular Function
    cysteine-type endopeptidase activity GO:0004197 IMP
    cysteine-type deubiquitinase activity GO:0004843 IDA
    cysteine-type deubiquitinase activity GO:0004843 TAS
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 IEA
    histone binding GO:0042393 IDA
    histone H2B deubiquitinase activity GO:0140936 IDA
Biological Process
    mRNA splicing, via spliceosome GO:0000398 IMP
    chromatin remodeling GO:0006338 IEA
    proteolysis GO:0006508 IEA
    protein deubiquitination GO:0016579 IDA
    protein deubiquitination GO:0016579 TAS
    negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051898 IDA
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IC
 Experiment description of studies that identified USP49 in exosomes
1
Experiment ID 217
MISEV standards
EM
Biophysical techniques
TSG101|CD81|CD9|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for USP49
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CETN1  
Affinity Capture-MS Homo sapiens
2 FBXO45 200933
Affinity Capture-Western Homo sapiens
3 PPP6R3 55291
Affinity Capture-MS Homo sapiens
4 DUSP1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
5 HADHA 3030
Affinity Capture-MS Homo sapiens
6 WRNIP1 56897
Affinity Capture-MS Homo sapiens
7 SHPRH  
Affinity Capture-MS Homo sapiens
8 NUP85 79902
Affinity Capture-MS Homo sapiens
9 HIST2H2BE 8349
Biochemical Activity Homo sapiens
10 NR3C1 2908
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
11 BAG2 9532
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
12 IVNS1ABP  
Affinity Capture-MS Homo sapiens
13 SLC39A7 7922
Affinity Capture-MS Homo sapiens
14 FKBP5 2289
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
15 PPP5C 5536
Affinity Capture-MS Homo sapiens
16 CETN3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 USP44  
Affinity Capture-MS Homo sapiens
18 MSX1  
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
19 PPP6R2 9701
Affinity Capture-MS Homo sapiens
20 PAX9  
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
21 COPA 1314
Affinity Capture-MS Homo sapiens
22 PRKACA 5566
Affinity Capture-MS Homo sapiens
23 ANKRD28 23243
Affinity Capture-MS Homo sapiens
24 STIP1 10963
Affinity Capture-MS Homo sapiens
25 LRPPRC 10128
Affinity Capture-MS Homo sapiens
26 TMEM173  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
27 KRR1 11103
Affinity Capture-MS Homo sapiens
28 NAMPT 10135
Two-hybrid Homo sapiens
29 ANKRD52 283373
Affinity Capture-MS Homo sapiens
30 RUVBL1 8607
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
31 UBR5 51366
Affinity Capture-MS Homo sapiens
32 EZH2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
33 PSMC5 5705
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
34 TRIM35 23087
Two-hybrid Homo sapiens
35 IPO7 10527
Affinity Capture-MS Homo sapiens
36 F7 2155
Affinity Capture-MS Homo sapiens
37 DICER1  
Affinity Capture-MS Homo sapiens
38 YARS2  
Affinity Capture-MS Homo sapiens
39 RPN1 6184
Affinity Capture-MS Homo sapiens
40 HSPH1 10808
Affinity Capture-MS Homo sapiens
41 PPP6R1 22870
Affinity Capture-MS Homo sapiens
42 SHCBP1 79801
Affinity Capture-MS Homo sapiens
43 STAMBPL1  
Affinity Capture-MS Homo sapiens
44 MCM6 4175
Affinity Capture-MS Homo sapiens
45 CETN2 1069
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 ZNF774  
Two-hybrid Homo sapiens
47 IMPG1  
Affinity Capture-MS Homo sapiens
48 RBCK1  
Two-hybrid Homo sapiens
49 PPM1G 5496
Affinity Capture-MS Homo sapiens
50 PDCD2 5134
Affinity Capture-MS Homo sapiens
51 HUWE1 10075
Affinity Capture-MS Homo sapiens
52 IPO9 55705
Affinity Capture-MS Homo sapiens
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