Gene description for NUP85
Gene name nucleoporin 85kDa
Gene symbol NUP85
Other names/aliases FROUNT
Nup75
Species Homo sapiens
 Database cross references - NUP85
ExoCarta ExoCarta_79902
Vesiclepedia VP_79902
Entrez Gene 79902
HGNC 8734
MIM 170285
UniProt Q9BW27  
 NUP85 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 34887515    
 Gene ontology annotations for NUP85
Molecular Function
    protein binding GO:0005515 IPI
    structural constituent of nuclear pore GO:0017056 IBA
Biological Process
    mRNA export from nucleus GO:0006406 IBA
    protein import into nucleus GO:0006606 IBA
    nucleocytoplasmic transport GO:0006913 NAS
    lamellipodium assembly GO:0030032 IEA
    positive regulation of DNA-templated transcription GO:0045893 IBA
    macrophage chemotaxis GO:0048246 IEA
    nephron development GO:0072006 IMP
Subcellular Localization
    kinetochore GO:0000776 IDA
    nuclear envelope GO:0005635 IDA
    nuclear envelope GO:0005635 TAS
    nuclear pore GO:0005643 NAS
    nucleoplasm GO:0005654 IDA
    spindle GO:0005819 IEA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    membrane GO:0016020 HDA
    nuclear pore outer ring GO:0031080 IBA
    nuclear pore outer ring GO:0031080 IDA
    nuclear pore outer ring GO:0031080 NAS
    nuclear membrane GO:0031965 IEA
 Experiment description of studies that identified NUP85 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
 Protein-protein interactions for NUP85
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TGOLN2 10618
Affinity Capture-MS Homo sapiens
2 SLFN11 91607
Affinity Capture-MS Homo sapiens
3 RIT1 6016
Negative Genetic Homo sapiens
4 COIL  
Proximity Label-MS Homo sapiens
5 EEF1G 1937
Two-hybrid Homo sapiens
6 MFNG  
Affinity Capture-MS Homo sapiens
7 KPNB1 3837
Affinity Capture-MS Homo sapiens
8 CCDC183  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 C9orf78 51759
Affinity Capture-MS Homo sapiens
10 NUMA1 4926
Affinity Capture-MS Homo sapiens
11 PTPN1 5770
Proximity Label-MS Homo sapiens
12 PNKD  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 NUP160 23279
Co-fractionation Homo sapiens
14 APEX1 328
Affinity Capture-RNA Homo sapiens
15 KIAA1429 25962
Affinity Capture-MS Homo sapiens
16 UXS1 80146
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 NUP153 9972
Affinity Capture-MS Homo sapiens
18 PRC1 9055
Affinity Capture-MS Homo sapiens
19 OBSL1 23363
Affinity Capture-MS Homo sapiens
20 NUP98 4928
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
21 TACSTD2 4070
Affinity Capture-MS Homo sapiens
22 SEH1L 81929
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
23 SNX27 81609
Affinity Capture-MS Homo sapiens
24 CA14 23632
Affinity Capture-MS Homo sapiens
25 FAF2 23197
Affinity Capture-MS Homo sapiens
26 DVL2 1856
Affinity Capture-MS Homo sapiens
27 KIF14 9928
Affinity Capture-MS Homo sapiens
28 NUP155 9631
Proximity Label-MS Homo sapiens
29 ENPP6 133121
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 CD70 970
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 Rcc1  
Affinity Capture-MS Mus musculus
32 C19orf38  
Affinity Capture-MS Homo sapiens
33 PARK2  
Affinity Capture-MS Homo sapiens
34 MAPRE1 22919
Affinity Capture-MS Homo sapiens
35 COMTD1 118881
Affinity Capture-MS Homo sapiens
36 UBE2I 7329
Affinity Capture-MS Homo sapiens
37 RAN 5901
Affinity Capture-MS Homo sapiens
38 NTRK1 4914
Affinity Capture-MS Homo sapiens
39 DTX2 113878
Proximity Label-MS Homo sapiens
40 CLSTN2 64084
Affinity Capture-MS Homo sapiens
41 PKN1 5585
Affinity Capture-MS Homo sapiens
42 EED  
Affinity Capture-MS Homo sapiens
43 Ube2i  
Affinity Capture-MS Mus musculus
44 GET4 51608
Two-hybrid Homo sapiens
45 SEC61B 10952
Proximity Label-MS Homo sapiens
46 CD40 958
Affinity Capture-MS Homo sapiens
47 DCTN3 11258
Co-fractionation Homo sapiens
48 NUP37 79023
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
49 Nup98  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
50 BTNL9  
Affinity Capture-MS Homo sapiens
51 XPO1 7514
Affinity Capture-MS Homo sapiens
52 LMNB1 4001
Proximity Label-MS Homo sapiens
53 SEC13 6396
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 NPTN 27020
Affinity Capture-MS Homo sapiens
55 CD274 29126
Affinity Capture-MS Homo sapiens
56 CUL3 8452
Affinity Capture-MS Homo sapiens
57 EGFR 1956
Affinity Capture-MS Homo sapiens
58 NUP43 348995
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 FBXO6 26270
Affinity Capture-MS Homo sapiens
60 NUP214 8021
Affinity Capture-MS Homo sapiens
61 NIFK 84365
Proximity Label-MS Homo sapiens
62 FKBP5 2289
Affinity Capture-MS Homo sapiens
63 GBF1 8729
Co-fractionation Homo sapiens
64 Nup107  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
65 SPAST 6683
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 RANBP1 5902
Affinity Capture-MS Homo sapiens
67 FOXQ1  
Affinity Capture-MS Homo sapiens
68 TNFSF13B  
Affinity Capture-MS Homo sapiens
69 CETN2 1069
Affinity Capture-Western Homo sapiens
70 NUP133 55746
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 HUWE1 10075
Affinity Capture-MS Homo sapiens
72 IKBKAP 8518
Co-fractionation Homo sapiens
73 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
74 PSMB10 5699
Affinity Capture-MS Homo sapiens
75 ZNF785  
Affinity Capture-MS Homo sapiens
76 SIRT6  
Affinity Capture-MS Homo sapiens
77 PARP1 142
Proximity Label-MS Homo sapiens
78 BKRF1  
Affinity Capture-MS
79 USP49 25862
Affinity Capture-MS Homo sapiens
80 USP36  
Affinity Capture-MS Homo sapiens
81 MEN1 4221
Affinity Capture-MS Homo sapiens
82 EFNB1 1947
Affinity Capture-MS Homo sapiens
83 RABL6 55684
Co-fractionation Homo sapiens
84 MKI67  
Affinity Capture-MS Homo sapiens
85 RGPD1  
Affinity Capture-MS Homo sapiens
86 Nup214  
Affinity Capture-MS Mus musculus
87 NUP50 10762
Proximity Label-MS Homo sapiens
88 NUP107 57122
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
89 SPN 6693
Affinity Capture-MS Homo sapiens
90 Ranbp2  
Affinity Capture-MS Mus musculus
91 KRAS 3845
Negative Genetic Homo sapiens
92 C9orf72  
Affinity Capture-MS Homo sapiens
93 VASN 114990
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
94 PSMC4 5704
Co-fractionation Homo sapiens
95 PLD6  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which NUP85 is involved
PathwayEvidenceSource
Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal TAS Reactome
Amplification of signal from the kinetochores TAS Reactome
Antiviral mechanism by IFN-stimulated genes TAS Reactome
Cell Cycle TAS Reactome
Cell Cycle IEA Reactome
Cell Cycle Checkpoints TAS Reactome
Cell Cycle, Mitotic TAS Reactome
Cell Cycle, Mitotic IEA Reactome
Cellular response to heat stress TAS Reactome
Cellular responses to stimuli TAS Reactome
Cellular responses to stress TAS Reactome
Cytokine Signaling in Immune system TAS Reactome
Defective TPR may confer susceptibility towards thyroid papillary carcinoma (TPC) TAS Reactome
Disease TAS Reactome
Disease IEA Reactome
Disorders of transmembrane transporters TAS Reactome
EML4 and NUDC in mitotic spindle formation TAS Reactome
Export of Viral Ribonucleoproteins from Nucleus TAS Reactome
Gene expression (Transcription) TAS Reactome
Gene Silencing by RNA TAS Reactome
Glucose metabolism TAS Reactome
Glycolysis TAS Reactome
HCMV Early Events TAS Reactome
HCMV Infection TAS Reactome
HCMV Late Events TAS Reactome
HIV Infection IEA Reactome
HIV Infection TAS Reactome
HIV Life Cycle TAS Reactome
Host Interactions of HIV factors IEA Reactome
Host Interactions of HIV factors TAS Reactome
Immune System TAS Reactome
Infectious disease TAS Reactome
Infectious disease IEA Reactome
Influenza Infection TAS Reactome
Influenza Viral RNA Transcription and Replication TAS Reactome
Interactions of Rev with host cellular proteins IEA Reactome
Interactions of Rev with host cellular proteins TAS Reactome
Interactions of Vpr with host cellular proteins TAS Reactome
Interferon Signaling TAS Reactome
ISG15 antiviral mechanism TAS Reactome
Late Phase of HIV Life Cycle TAS Reactome
M Phase TAS Reactome
M Phase IEA Reactome
Metabolism TAS Reactome
Metabolism of carbohydrates TAS Reactome
Metabolism of non-coding RNA TAS Reactome
Metabolism of proteins TAS Reactome
Metabolism of proteins IEA Reactome
Metabolism of RNA TAS Reactome
Mitotic Anaphase TAS Reactome
Mitotic Anaphase IEA Reactome
Mitotic Metaphase and Anaphase TAS Reactome
Mitotic Metaphase and Anaphase IEA Reactome
Mitotic Prometaphase TAS Reactome
Mitotic Prophase TAS Reactome
Mitotic Spindle Checkpoint TAS Reactome
NEP/NS2 Interacts with the Cellular Export Machinery TAS Reactome
NS1 Mediated Effects on Host Pathways TAS Reactome
Nuclear Envelope (NE) Reassembly TAS Reactome
Nuclear Envelope (NE) Reassembly IEA Reactome
Nuclear Envelope Breakdown TAS Reactome
Nuclear import of Rev protein IEA Reactome
Nuclear Pore Complex (NPC) Disassembly TAS Reactome
Post-translational protein modification TAS Reactome
Post-translational protein modification IEA Reactome
Postmitotic nuclear pore complex (NPC) reformation TAS Reactome
Postmitotic nuclear pore complex (NPC) reformation IEA Reactome
Processing of Capped Intron-Containing Pre-mRNA TAS Reactome
Regulation of Glucokinase by Glucokinase Regulatory Protein TAS Reactome
Regulation of HSF1-mediated heat shock response TAS Reactome
Resolution of Sister Chromatid Cohesion TAS Reactome
Rev-mediated nuclear export of HIV RNA TAS Reactome
RHO GTPase Effectors TAS Reactome
RHO GTPases Activate Formins TAS Reactome
SARS-CoV Infections TAS Reactome
SARS-CoV-2 activates/modulates innate and adaptive immune responses TAS Reactome
SARS-CoV-2 Infection TAS Reactome
SARS-CoV-2-host interactions TAS Reactome
Separation of Sister Chromatids TAS Reactome
Signal Transduction TAS Reactome
Signaling by Rho GTPases TAS Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 TAS Reactome
SLC transporter disorders TAS Reactome
snRNP Assembly TAS Reactome
SUMO E3 ligases SUMOylate target proteins TAS Reactome
SUMO E3 ligases SUMOylate target proteins IEA Reactome
SUMOylation TAS Reactome
SUMOylation IEA Reactome
SUMOylation of chromatin organization proteins TAS Reactome
SUMOylation of DNA damage response and repair proteins TAS Reactome
SUMOylation of DNA replication proteins IEA Reactome
SUMOylation of DNA replication proteins TAS Reactome
SUMOylation of RNA binding proteins TAS Reactome
SUMOylation of SUMOylation proteins TAS Reactome
SUMOylation of ubiquitinylation proteins TAS Reactome
Transcriptional regulation by small RNAs TAS Reactome
Transport of Mature mRNA derived from an Intron-Containing Transcript TAS Reactome
Transport of Mature mRNA Derived from an Intronless Transcript TAS Reactome
Transport of Mature mRNAs Derived from Intronless Transcripts TAS Reactome
Transport of Mature Transcript to Cytoplasm TAS Reactome
Transport of Ribonucleoproteins into the Host Nucleus TAS Reactome
Transport of the SLBP Dependant Mature mRNA TAS Reactome
Transport of the SLBP independent Mature mRNA TAS Reactome
tRNA processing TAS Reactome
tRNA processing in the nucleus TAS Reactome
Viral Infection Pathways TAS Reactome
Viral Infection Pathways IEA Reactome
Viral Messenger RNA Synthesis TAS Reactome
Vpr-mediated nuclear import of PICs TAS Reactome





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