Gene description for GBF1
Gene name golgi brefeldin A resistant guanine nucleotide exchange factor 1
Gene symbol GBF1
Other names/aliases ARF1GEF
Species Homo sapiens
 Database cross references - GBF1
ExoCarta ExoCarta_8729
Vesiclepedia VP_8729
Entrez Gene 8729
HGNC 4181
MIM 603698
UniProt Q92538  
 GBF1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Prostate cancer cells 25844599    
 Gene ontology annotations for GBF1
Molecular Function
    guanyl-nucleotide exchange factor activity GO:0005085 TAS
    protein binding GO:0005515 IPI
    phosphatidylinositol-3,4,5-trisphosphate binding GO:0005547 IDA
    phosphatidylinositol-3,5-bisphosphate binding GO:0080025 IDA
Biological Process
    cell activation involved in immune response GO:0002263 IMP
    endoplasmic reticulum to Golgi vesicle-mediated transport GO:0006888 TAS
    retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum GO:0006890 IMP
    post-Golgi vesicle-mediated transport GO:0006892 TAS
    Golgi to endosome transport GO:0006895 IMP
    Golgi organization GO:0007030 IMP
    regulation of mitotic cell cycle GO:0007346 IMP
    protein transport GO:0015031 IEA
    neutrophil chemotaxis GO:0030593 IMP
    regulation of ARF protein signal transduction GO:0032012 IEA
    protein localization to Golgi apparatus GO:0034067 IMP
    retrograde transport, endosome to Golgi GO:0042147 IMP
    COPI coating of Golgi vesicle GO:0048205 IMP
    cilium assembly GO:0060271 TAS
    establishment of monopolar cell polarity GO:0061162 IMP
    protein localization to endoplasmic reticulum exit site GO:0070973 IMP
    Golgi disassembly GO:0090166 IMP
    endoplasmic reticulum-Golgi intermediate compartment organization GO:0097111 IMP
    cellular response to virus GO:0098586 IMP
    reactive oxygen species biosynthetic process GO:1903409 IMP
    protein localization to endoplasmic reticulum tubular network GO:1903420 IMP
    regulation of protein localization to cell surface GO:2000008 IMP
Subcellular Localization
    Golgi membrane GO:0000139 TAS
    peroxisome GO:0005777 IEA
    endoplasmic reticulum lumen GO:0005788 IEA
    endoplasmic reticulum-Golgi intermediate compartment GO:0005793 IDA
    Golgi apparatus GO:0005794 IDA
    Golgi stack GO:0005795 IEA
    cis-Golgi network GO:0005801 IDA
    trans-Golgi network GO:0005802 IDA
    lipid droplet GO:0005811 IEA
    cytosol GO:0005829 TAS
    membrane GO:0016020 HDA
    cell leading edge GO:0031252 IDA
 Experiment description of studies that identified GBF1 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 207
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 208
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 209
MISEV standards
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 211
MISEV standards
EM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
cytochrome c|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 212
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
Cytochrome C|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 275
MISEV standards
EM
Biophysical techniques
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
AIF
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
 Protein-protein interactions for GBF1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GJD3  
Proximity Label-MS Homo sapiens
2 C5AR2  
Affinity Capture-MS Homo sapiens
3 LYPD3 27076
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 FBXO7 25793
Affinity Capture-MS Homo sapiens
5 METTL7A 25840
Proximity Label-MS Homo sapiens
6 GJA1 2697
Proximity Label-MS Homo sapiens
7 HCST  
Affinity Capture-MS Homo sapiens
8 DLK2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 YWHAE 7531
Affinity Capture-MS Homo sapiens
10 TMEM206 55248
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 SLC2A1 6513
Affinity Capture-MS Homo sapiens
12 FPR1  
Affinity Capture-MS Homo sapiens
13 TYROBP  
Affinity Capture-MS Homo sapiens
14 UBC 7316
Reconstituted Complex Homo sapiens
15 MILR1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 FFAR1  
Affinity Capture-MS Homo sapiens
17 PNKD  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 KIAA1429 25962
Affinity Capture-MS Homo sapiens
19 PARK2  
Affinity Capture-MS Homo sapiens
20 B3GAT1  
Proximity Label-MS Homo sapiens
21 B3GNT2 10678
Affinity Capture-MS Homo sapiens
22 DYRK1A 1859
Affinity Capture-MS Homo sapiens
23 VARS 7407
Co-fractionation Homo sapiens
24 HLA-B 3106
Affinity Capture-MS Homo sapiens
25 FAM174A 345757
Affinity Capture-MS Homo sapiens
26 RAB3B 5865
Proximity Label-MS Homo sapiens
27 PRC1 9055
Affinity Capture-MS Homo sapiens
28 CLEC2B  
Affinity Capture-MS Homo sapiens
29 RNF133  
Affinity Capture-MS Homo sapiens
30 YWHAG 7532
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 EPS15L1 58513
Co-fractionation Homo sapiens
32 EPHA1 2041
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 STX6 10228
Proximity Label-MS Homo sapiens
34 ITFG3 83986
Affinity Capture-MS Homo sapiens
35 YWHAH 7533
Affinity Capture-MS Homo sapiens
36 F2RL1  
Affinity Capture-MS Homo sapiens
37 BTRC 8945
Affinity Capture-MS Homo sapiens
38 Ccser2  
Affinity Capture-MS Mus musculus
39 EBAG9 9166
Proximity Label-MS Homo sapiens
40 GPR17 2840
Affinity Capture-MS Homo sapiens
41 KIF14 9928
Affinity Capture-MS Homo sapiens
42 YWHAB 7529
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 BSG 682
Affinity Capture-MS Homo sapiens
44 CD70 970
Affinity Capture-MS Homo sapiens
45 IRS4 8471
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
46 COPG1  
Affinity Capture-Western Bos taurus
47 CUL3 8452
Affinity Capture-MS Homo sapiens
48 CTDP1 9150
Affinity Capture-MS Homo sapiens
49 FOXI2  
Affinity Capture-MS Homo sapiens
50 GPR55  
Affinity Capture-MS Homo sapiens
51 CCAR2 57805
Co-fractionation Homo sapiens
52 ITGAD  
Affinity Capture-MS Homo sapiens
53 P2RY8  
Affinity Capture-MS Homo sapiens
54 COPG1 22820
Affinity Capture-Western Homo sapiens
55 VSIG4  
Affinity Capture-MS Homo sapiens
56 COMTD1 118881
Affinity Capture-MS Homo sapiens
57 VCP 7415
Affinity Capture-MS Homo sapiens
58 NTRK1 4914
Affinity Capture-MS Homo sapiens
59 GPR182  
Affinity Capture-MS Homo sapiens
60 ZSCAN26  
Affinity Capture-MS Homo sapiens
61 GML  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 ENO1 2023
Cross-Linking-MS (XL-MS) Homo sapiens
63 ZYG11B  
Two-hybrid Homo sapiens
64 ELOVL5 60481
Proximity Label-MS Homo sapiens
65 SLC2A9  
Affinity Capture-MS Homo sapiens
66 UPK2 7379
Affinity Capture-MS Homo sapiens
67 CD40 958
Affinity Capture-MS Homo sapiens
68 S1PR1 1901
Affinity Capture-MS Homo sapiens
69 CYB5B 80777
Affinity Capture-MS Homo sapiens
70 EFNA4  
Affinity Capture-MS Homo sapiens
71 AIFM1 9131
Affinity Capture-MS Homo sapiens
72 CDC73  
Affinity Capture-MS Homo sapiens
73 RAB5A 5868
Proximity Label-MS Homo sapiens
74 NPTN 27020
Affinity Capture-MS Homo sapiens
75 CHRNA1  
Affinity Capture-MS Homo sapiens
76 C17orf78  
Affinity Capture-MS Homo sapiens
77 MGARP  
Affinity Capture-MS Homo sapiens
78 FOXG1  
Affinity Capture-MS Homo sapiens
79 YWHAZ 7534
Affinity Capture-MS Homo sapiens
80 NPY2R  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
81 EGFR 1956
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
82 FOXL1  
Affinity Capture-MS Homo sapiens
83 KBTBD7  
Affinity Capture-MS Homo sapiens
84 RIPK4  
Affinity Capture-MS Homo sapiens
85 SPACA1 81833
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
86 OCIAD1 54940
Affinity Capture-MS Homo sapiens
87 AURKB 9212
Affinity Capture-MS Homo sapiens
88 BIRC6 57448
Co-fractionation Homo sapiens
89 NCAPH 23397
Co-fractionation Homo sapiens
90 RBM26 64062
Co-fractionation Homo sapiens
91 C1orf210  
Affinity Capture-MS Homo sapiens
92 BAG1 573
Affinity Capture-MS Homo sapiens
93 GPR45  
Affinity Capture-MS Homo sapiens
94 LYPD1  
Affinity Capture-MS Homo sapiens
95 GOLGA1  
Proximity Label-MS Homo sapiens
96 P2RY10  
Affinity Capture-MS Homo sapiens
97 HLA-DQB1 3119
Affinity Capture-MS Homo sapiens
98 LPPR2  
Affinity Capture-MS Homo sapiens
99 PNPLA2  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
100 HIF1A 3091
Affinity Capture-MS Homo sapiens
101 ST3GAL5  
Affinity Capture-MS Homo sapiens
102 TUBB2A 7280
Affinity Capture-MS Homo sapiens
103 GYPA  
Affinity Capture-MS Homo sapiens
104 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
105 FBXW11  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
106 PUS7 54517
Co-fractionation Homo sapiens
107 STK16 8576
Affinity Capture-MS Homo sapiens
108 TMEM9 252839
Affinity Capture-MS Homo sapiens
109 PLEC 5339
Co-fractionation Homo sapiens
110 VOPP1  
Affinity Capture-MS Homo sapiens
111 MFSD4  
Affinity Capture-MS Homo sapiens
112 FOXB1  
Affinity Capture-MS Homo sapiens
113 CHRM4  
Affinity Capture-MS Homo sapiens
114 RABL6 55684
Co-fractionation Homo sapiens
115 CHRM3 1131
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
116 EVA1C  
Affinity Capture-MS Homo sapiens
117 ERGIC2 51290
Proximity Label-MS Homo sapiens
118 RAB2A 5862
Proximity Label-MS Homo sapiens
119 SPN 6693
Affinity Capture-MS Homo sapiens
120 FIS1 51024
Affinity Capture-MS Homo sapiens
121 RAB7A 7879
Proximity Label-MS Homo sapiens
122 RAB11A 8766
Proximity Label-MS Homo sapiens
123 TPST2 8459
Affinity Capture-MS Homo sapiens
124 C9orf72  
Affinity Capture-MS Homo sapiens
125 FXYD3 5349
Affinity Capture-MS Homo sapiens
126 NUP85 79902
Co-fractionation Homo sapiens
127 IVNS1ABP  
Affinity Capture-MS Homo sapiens
128 RPA3 6119
Proximity Label-MS Homo sapiens
129 CAV1 857
Proximity Label-MS Homo sapiens
130 KIF4A 24137
Co-fractionation Homo sapiens
131 CUL4A 8451
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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