Gene description for GBF1
Gene name golgi brefeldin A resistant guanine nucleotide exchange factor 1
Gene symbol GBF1
Other names/aliases ARF1GEF
Species Homo sapiens
 Database cross references - GBF1
ExoCarta ExoCarta_8729
Entrez Gene 8729
HGNC 4181
MIM 603698
UniProt Q92538  
 GBF1 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Prostate cancer cells 25844599    
 Gene ontology annotations for GBF1
Molecular Function
    phosphatidylinositol-3,5-bisphosphate binding GO:0080025 IDA
    phosphatidylinositol-3,4,5-trisphosphate binding GO:0005547 IDA
    ARF guanyl-nucleotide exchange factor activity GO:0005086 IBA
    protein binding GO:0005515 IPI
Biological Process
    protein localization to endoplasmic reticulum tubular network GO:1903420 IMP
    Golgi disassembly GO:0090166 IMP
    COPI coating of Golgi vesicle GO:0048205 TAS
    Golgi to endosome transport GO:0006895 IMP
    retrograde vesicle-mediated transport, Golgi to ER GO:0006890 TAS
    regulation of cell adhesion GO:0030155 IBA
    endoplasmic reticulum-Golgi intermediate compartment organization GO:0097111 IMP
    positive regulation of GTPase activity GO:0043547 IEA
    protein localization to endoplasmic reticulum exit site GO:0070973 IMP
    retrograde transport, endosome to Golgi GO:0042147 IMP
    regulation of mitotic cell cycle GO:0007346 IMP
    neutrophil chemotaxis GO:0030593 IMP
    organelle organization GO:0006996 TAS
    establishment of monopolar cell polarity GO:0061162 IMP
    membrane organization GO:0061024 TAS
    protein localization to Golgi apparatus GO:0034067 IMP
    Golgi organization GO:0007030 IMP
    post-Golgi vesicle-mediated transport GO:0006892 TAS
    protein transport GO:0015031 IEA
    cell activation involved in immune response GO:0002263 IMP
    viral process GO:0016032 IEA
    regulation of protein localization to cell surface GO:2000008 IMP
    reactive oxygen species biosynthetic process GO:1903409 IMP
Subcellular Localization
    mitochondrion GO:0005739 IEA
    Golgi apparatus GO:0005794 IDA
    trans-Golgi network GO:0005802 IDA
    membrane GO:0016020 IDA
    lipid particle GO:0005811 IEA
    cell leading edge GO:0031252 IDA
    Golgi membrane GO:0000139 TAS
    Golgi stack GO:0005795 IEA
    endoplasmic reticulum lumen GO:0005788 IEA
    peroxisome GO:0005777 IEA
    cis-Golgi network GO:0005801 IDA
    endoplasmic reticulum-Golgi intermediate compartment GO:0005793 IDA
    plasma membrane GO:0005886 IBA
 Experiment description of studies that identified GBF1 in exosomes
1
Experiment ID 207
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
✘ EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 275
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|RAB5A
EV Cytosolic markers
✔
CD9|CD82|CD63|CD81
EV Membrane markers
✔
AIF
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
 Protein-protein interactions for GBF1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAG 7532
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which GBF1 is involved
PathwayEvidenceSource
Clathrin derived vesicle budding TAS Reactome
COPI Mediated Transport TAS Reactome
VxPx cargo-targeting to cilium TAS Reactome





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