Gene description for CCAR2
Gene name cell cycle and apoptosis regulator 2
Gene symbol CCAR2
Other names/aliases DBC-1
DBC1
KIAA1967
NET35
p30 DBC
p30DBC
Species Homo sapiens
 Database cross references - CCAR2
ExoCarta ExoCarta_57805
Entrez Gene 57805
HGNC 23360
MIM 607359
UniProt Q8N163  
 CCAR2 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for CCAR2
Molecular Function
    enzyme inhibitor activity GO:0004857 IMP
    enzyme binding GO:0019899 IPI
    poly(A) RNA binding GO:0044822 IDA
    protein binding GO:0005515 IPI
    RNA polymerase II core binding GO:0000993 IDA
Biological Process
    negative regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032435 IDA
    negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage GO:1902230 IMP
    cell cycle GO:0007049 IEA
    regulation of cellular response to heat GO:1900034 TAS
    regulation of circadian rhythm GO:0042752 ISS
    regulation of protein stability GO:0031647 IDA
    positive regulation of DNA damage checkpoint GO:2000003 IMP
    mitochondrial fragmentation involved in apoptotic process GO:0043653 IDA
    regulation of DNA-templated transcription, elongation GO:0032784 IMP
    positive regulation of apoptotic process GO:0043065 IMP
    RNA splicing GO:0008380 IMP
    cellular response to heat GO:0034605 TAS
    rhythmic process GO:0048511 IEA
    mRNA processing GO:0006397 IEA
    response to UV GO:0009411 IMP
    negative regulation of catalytic activity GO:0043086 IMP
    cellular response to DNA damage stimulus GO:0006974 IEA
Subcellular Localization
    nucleus GO:0005634 IDA
    spliceosomal complex GO:0005681 IDA
    mitochondrial matrix GO:0005759 IDA
    nucleoplasm GO:0005654 TAS
    DBIRD complex GO:0044609 IDA
    cytoplasm GO:0005737 IDA
    nuclear chromatin GO:0000790 IDA
 Experiment description of studies that identified CCAR2 in exosomes
1
Experiment ID 207
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
✘ EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CCAR2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NELFCD 51497
Affinity Capture-MS Homo sapiens
2 MAGED1  
Affinity Capture-MS Homo sapiens
3 CCNA1  
Affinity Capture-MS Homo sapiens
4 MAGEA6  
Affinity Capture-MS Homo sapiens
5 NEK6 10783
Affinity Capture-MS Homo sapiens
6 WDR8  
Affinity Capture-MS Homo sapiens
7 ACTR3B 57180
Affinity Capture-MS Homo sapiens
8 MYC  
Affinity Capture-MS Homo sapiens
9 CIAO1 9391
Affinity Capture-MS Homo sapiens
10 GSTK1 373156
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which CCAR2 is involved
PathwayEvidenceSource
Regulation of HSF1-mediated heat shock response TAS Reactome





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