Gene description for SMEK1
Gene name SMEK homolog 1, suppressor of mek1 (Dictyostelium)
Gene symbol SMEK1
Other names/aliases FLFL1
KIAA2010
MSTP033
PP4R3A
smk-1
smk1
Species Homo sapiens
 Database cross references - SMEK1
ExoCarta ExoCarta_55671
Vesiclepedia VP_55671
Entrez Gene 55671
HGNC 20219
MIM 610351
UniProt Q6IN85  
 SMEK1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for SMEK1
Molecular Function
    protein phosphatase activator activity GO:0072542 IBA
Biological Process
    DNA damage response GO:0006974 IBA
    regulation of double-strand break repair GO:2000779 IBA
Subcellular Localization
    nucleoplasm GO:0005654 IBA
    nucleoplasm GO:0005654 IDA
    centrosome GO:0005813 IEA
    cytosol GO:0005829 IDA
    nuclear speck GO:0016607 IDA
    protein phosphatase 4 complex GO:0030289 IBA
 Experiment description of studies that identified SMEK1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for SMEK1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SNRPB2 6629
Affinity Capture-MS Homo sapiens
2 RNGTT 8732
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 MCM7 4176
Co-fractionation Homo sapiens
4 SNX3 8724
Co-fractionation Homo sapiens
5 PPP2R4 5524
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 DNAJC8 22826
Affinity Capture-MS Homo sapiens
7 UBXN10  
Affinity Capture-MS Homo sapiens
8 C9orf78 51759
Affinity Capture-MS Homo sapiens
9 ARCN1 372
Co-fractionation Homo sapiens
10 HNRNPR 10236
Co-fractionation Homo sapiens
11 PRPF40A 55660
Co-fractionation Homo sapiens
12 SNRPF 6636
Affinity Capture-MS Homo sapiens
13 SNRPD2 6633
Affinity Capture-MS Homo sapiens
14 SF3B5 83443
Affinity Capture-MS Homo sapiens
15 APEX1 328
Affinity Capture-RNA Homo sapiens
16 RNH1 6050
Co-fractionation Homo sapiens
17 MCM2 4171
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
18 SF3B1 23451
Affinity Capture-MS Homo sapiens
19 COPB2 9276
Co-fractionation Homo sapiens
20 SPAG9 9043
Affinity Capture-MS Homo sapiens
21 OSBP 5007
Affinity Capture-MS Homo sapiens
22 TELO2 9894
Co-fractionation Homo sapiens
23 BRCC3 79184
Co-fractionation Homo sapiens
24 FBXO28  
Affinity Capture-MS Homo sapiens
25 ADCYAP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 OBSL1 23363
Affinity Capture-MS Homo sapiens
27 SMC2 10592
Co-fractionation Homo sapiens
28 CCDC6 8030
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 MARCKSL1 65108
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 TUBA3C 7278
Affinity Capture-MS Homo sapiens
31 RRP8  
Affinity Capture-MS Homo sapiens
32 SIGLEC12  
Affinity Capture-MS Homo sapiens
33 MAPK14 1432
Two-hybrid Homo sapiens
34 SNX27 81609
Affinity Capture-MS Homo sapiens
35 HUWE1 10075
Affinity Capture-MS Homo sapiens
36 PMPCB 9512
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 KNSTRN  
Affinity Capture-MS Homo sapiens
38 MTA2 9219
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
39 GDA 9615
Affinity Capture-MS Homo sapiens
40 CASK 8573
Two-hybrid Homo sapiens
41 MED1 5469
Affinity Capture-MS Homo sapiens
42 COBLL1 22837
Affinity Capture-MS Homo sapiens
43 ACAT2 39
Co-fractionation Homo sapiens
44 USP10 9100
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 CCAR2 57805
Co-fractionation Homo sapiens
46 SUPT5H 6829
Affinity Capture-MS Homo sapiens
47 EHD2 30846
Affinity Capture-MS Homo sapiens
48 Ppp4c 56420
Affinity Capture-MS Mus musculus
49 FAM19A3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 CAST 831
Co-fractionation Homo sapiens
51 ACACA 31
Co-fractionation Homo sapiens
52 COMTD1 118881
Affinity Capture-MS Homo sapiens
53 SNRPB 6628
Affinity Capture-MS Homo sapiens
54 RPL18A 6142
Affinity Capture-MS Homo sapiens
55 AMPD2 271
Co-fractionation Homo sapiens
56 NTRK1 4914
Affinity Capture-MS Homo sapiens
57 DEK 7913
Co-fractionation Homo sapiens
58 ALKBH3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 PPP1R21 129285
Affinity Capture-MS Homo sapiens
60 PPME1 51400
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 SF3B6 51639
Affinity Capture-MS Homo sapiens
62 RPA4  
Proximity Label-MS Homo sapiens
63 MYH10 4628
Co-fractionation Homo sapiens
64 SNRNP27  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 CEBPA  
Proximity Label-MS Homo sapiens
Protein-peptide Homo sapiens
66 CT45A5  
Affinity Capture-MS Homo sapiens
67 DACT2  
Affinity Capture-MS Homo sapiens
68 LRCH3 84859
Affinity Capture-MS Homo sapiens
69 IGBP1 3476
Co-fractionation Homo sapiens
70 SF3B2 10992
Affinity Capture-MS Homo sapiens
71 PPP4R2  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 SMEK2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
73 BUB1B  
Affinity Capture-MS Homo sapiens
74 USP15 9958
Affinity Capture-MS Homo sapiens
75 CCDC120  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 COG4 25839
Affinity Capture-MS Homo sapiens
77 PTPRA 5786
Affinity Capture-MS Homo sapiens
78 CUL3 8452
Affinity Capture-MS Homo sapiens
79 XAB2 56949
Affinity Capture-MS Homo sapiens
80 DDB2  
Co-fractionation Homo sapiens
81 PSMA2 5683
Co-fractionation Homo sapiens
82 NSFL1C 55968
Affinity Capture-MS Homo sapiens
83 MCM4 4173
Affinity Capture-MS Homo sapiens
84 TSSC4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
85 POLR2A 5430
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
86 DNAJA1 3301
Co-fractionation Homo sapiens
87 OCIAD1 54940
Affinity Capture-MS Homo sapiens
88 SLC4A1AP  
Affinity Capture-MS Homo sapiens
89 SF3A1 10291
Co-fractionation Homo sapiens
90 SCAF4 57466
Affinity Capture-MS Homo sapiens
91 RPA3 6119
Proximity Label-MS Homo sapiens
92 MTOR 2475
Co-fractionation Homo sapiens
93 EHMT2 10919
Co-fractionation Homo sapiens
94 CRAMP1L 57585
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
95 RHPN1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
96 SF3B3 23450
Affinity Capture-MS Homo sapiens
97 CACTIN  
Affinity Capture-MS Homo sapiens
98 PLA2G2E  
Affinity Capture-MS Homo sapiens
99 GRK6 2870
Affinity Capture-MS Homo sapiens
100 PSMB1 5689
Co-fractionation Homo sapiens
101 SIRT1  
Affinity Capture-MS Homo sapiens
102 FAM133A 286499
Affinity Capture-MS Homo sapiens
103 MRPL42  
Affinity Capture-MS Homo sapiens
104 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
105 PPP4C 5531
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
106 CLK1  
Affinity Capture-MS Homo sapiens
107 ZNF785  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
108 SIRT6  
Affinity Capture-MS Homo sapiens
109 TMEM69  
Affinity Capture-MS Homo sapiens
110 CPSF6 11052
Co-fractionation Homo sapiens
111 DOCK5 80005
Affinity Capture-MS Homo sapiens
112 RBM25 58517
Co-fractionation Homo sapiens
113 SF3B4 10262
Affinity Capture-MS Homo sapiens
114 HDAC1 3065
Affinity Capture-Western Homo sapiens
115 FAM120AOS  
Affinity Capture-MS Homo sapiens
116 FKBP1A 2280
Co-fractionation Homo sapiens
117 DDX58 23586
Affinity Capture-RNA Homo sapiens
118 LRRTM4  
Affinity Capture-MS Homo sapiens
119 ITSN2 50618
Affinity Capture-MS Homo sapiens
120 GAPDH 2597
Co-fractionation Homo sapiens
121 DHX38 9785
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
122 EHMT1  
Co-fractionation Homo sapiens
123 SNX12 29934
Co-fractionation Homo sapiens
124 SNRPA1 6627
Affinity Capture-MS Homo sapiens
125 PVRL3 25945
Affinity Capture-MS Homo sapiens
126 WDHD1  
Co-fractionation Homo sapiens
View the network image/svg+xml
 Pathways in which SMEK1 is involved
No pathways found





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