Experiment description of studies that identified COBLL1 in sEVs

1 Experiment ID 236 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocellular carcinoma cells Sample name MHCC97L Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RT-PCR RNA Sequencing

2 Experiment ID 211 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|EpCAM|TFRC Enriched markers ✔ cytochrome c|GOLGA2 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23333927     Organism Homo sapiens Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes. Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K." Journal name J Proteomics Publication year 2013 Sample Ovarian cancer cells Sample name IGROV1 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.09-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

3 Experiment ID 212 MISEV standards ✔ CEM Biophysical techniques ✔ TSG101|Alix|EpCAM|TFRC Enriched markers ✔ Cytochrome C|GOLGA2 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23333927     Organism Homo sapiens Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes. Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K." Journal name J Proteomics Publication year 2013 Sample Ovarian cancer cells Sample name OVCAR-3 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.09-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

4 Experiment ID 834 MISEV standards ✔ EM Biophysical techniques ✔ LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

5 Experiment ID 835 MISEV standards ✔ EM Biophysical techniques ✔ CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

6 Experiment ID 63 MISEV standards ✘ Biophysical techniques ✔ AQP2 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 19056867     Organism Homo sapiens Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes. Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA" Journal name JASN Publication year 2009 Sample Urine Sample name Urine - Normal Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] Western blotting

Protein-protein interactions for COBLL1

Protein Interactor ExoCarta ID Identification method PubMed Species 1 YWHAH 7533 Affinity Capture-MS Homo sapiens 2 ILF3 3609 Affinity Capture-MS Homo sapiens 3 UHRF1BP1L 23074 Affinity Capture-MS Homo sapiens 4 AMOTL1 154810 Affinity Capture-MS Homo sapiens 5 PACSIN1   Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 6 DVL2 1856 Affinity Capture-MS Homo sapiens 7 KIAA1107   Affinity Capture-MS Homo sapiens 8 MARCKS 4082 Proximity Label-MS Homo sapiens 9 HSPA8 3312 Co-fractionation Homo sapiens 10 DLG3 1741 Affinity Capture-MS Homo sapiens 11 YWHAB 7529 Affinity Capture-MS Homo sapiens 12 SMARCA5 8467 Co-fractionation Homo sapiens 13 TLK2   Affinity Capture-MS Homo sapiens 14 Dync1li1 235661 Affinity Capture-MS Mus musculus 15 PFN1 5216 Proximity Label-MS Homo sapiens 16 DDHD1 80821 Affinity Capture-MS Homo sapiens 17 YWHAE 7531 Affinity Capture-MS Homo sapiens 18 SCML1   Affinity Capture-MS Homo sapiens 19 EZR 7430 Proximity Label-MS Homo sapiens 20 ACTC1 70 Proximity Label-MS Homo sapiens 21 Cep135   Affinity Capture-MS Mus musculus 22 PACSIN2 11252 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 23 LCK 3932 Proximity Label-MS Homo sapiens 24 YWHAZ 7534 Affinity Capture-MS Homo sapiens 25 IFFO1   Affinity Capture-MS Homo sapiens 26 PACSIN3 29763 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 27 HSP90AA1 3320 Affinity Capture-MS Homo sapiens 28 HMBOX1   Affinity Capture-MS Homo sapiens 29 SMEK1 55671 Affinity Capture-MS Homo sapiens 30 LYN 4067 Proximity Label-MS Homo sapiens 31 HSPA5 3309 Co-fractionation Homo sapiens 32 Rassf1   Affinity Capture-MS Mus musculus 33 ITGB3BP   Affinity Capture-MS Homo sapiens 34 CAV1 857 Proximity Label-MS Homo sapiens 35 Tgs1   Affinity Capture-MS Mus musculus 36 CDH1 999 Proximity Label-MS Homo sapiens 37 NRD1 4898 Affinity Capture-MS Homo sapiens 38 AI314180 230249 Affinity Capture-MS Mus musculus 39 SORT1 6272 Affinity Capture-MS Homo sapiens 40 RDX 5962 Proximity Label-MS Homo sapiens 41 ACTB 60 Proximity Label-MS Homo sapiens 42 HIST1H3A 8350 Cross-Linking-MS (XL-MS) Homo sapiens 43 YWHAG 7532 Affinity Capture-MS Homo sapiens 44 CDADC1   Affinity Capture-MS Homo sapiens 45 AK1 203 Affinity Capture-MS Homo sapiens 46 ACTG1 71 Affinity Capture-MS Homo sapiens 47 OCLN 100506658 Proximity Label-MS Homo sapiens 48 FGFR1OP   Affinity Capture-MS Homo sapiens 49 Homer1   Affinity Capture-MS Mus musculus 50 FAM117A 81558 Affinity Capture-MS Homo sapiens View the network

Pathways in which COBLL1 is involved

No pathways found