Gene description for AMOTL1
Gene name angiomotin like 1
Gene symbol AMOTL1
Other names/aliases JEAP
Species Homo sapiens
 Database cross references - AMOTL1
ExoCarta ExoCarta_154810
Vesiclepedia VP_154810
Entrez Gene 154810
HGNC 17811
MIM 614657
UniProt Q8IY63  
 AMOTL1 identified in exosomes derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
 Gene ontology annotations for AMOTL1
Molecular Function
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IDA
Biological Process
    angiogenesis GO:0001525 IBA
    establishment of cell polarity involved in ameboidal cell migration GO:0003365 IBA
    Wnt signaling pathway GO:0016055 IEA
    actin cytoskeleton organization GO:0030036 IBA
    regulation of cell migration GO:0030334 IBA
    hippo signaling GO:0035329 IBA
Subcellular Localization
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IBA
    bicellular tight junction GO:0005923 IBA
    bicellular tight junction GO:0005923 IDA
    COP9 signalosome GO:0008180 IDA
    cytoplasmic vesicle GO:0031410 IBA
 Experiment description of studies that identified AMOTL1 in exosomes
1
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for AMOTL1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAE 7531
Affinity Capture-MS Homo sapiens
2 ANLN 54443
Affinity Capture-MS Homo sapiens
3 YPEL5 51646
Affinity Capture-MS Homo sapiens
4 Magi1  
Affinity Capture-Western Mus musculus
5 Wwtr1  
Affinity Capture-MS Mus musculus
6 TFCP2 7024
Affinity Capture-MS Homo sapiens
7 Lin7c 22343
Affinity Capture-MS Mus musculus
8 DYNLL2 140735
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 MRPS2 51116
Affinity Capture-MS Homo sapiens
10 NEDD4 4734
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
11 MRPS9 64965
Affinity Capture-MS Homo sapiens
12 WWTR1 25937
Affinity Capture-MS Homo sapiens
13 KIF14 9928
Affinity Capture-MS Homo sapiens
14 WWP1 11059
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 MRPS35 60488
Affinity Capture-MS Homo sapiens
16 TNKS 8658
Affinity Capture-Luminescence Homo sapiens
17 WWOX 51741
Affinity Capture-MS Homo sapiens
18 TLK2  
Affinity Capture-MS Homo sapiens
19 COBLL1 22837
Affinity Capture-MS Homo sapiens
20 SQSTM1 8878
Proximity Label-MS Homo sapiens
21 DDHD1 80821
Affinity Capture-MS Homo sapiens
22 PARD3 56288
Proximity Label-MS Homo sapiens
23 NF2 4771
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
24 CTNNB1 1499
Proximity Label-MS Homo sapiens
25 Amot  
Affinity Capture-MS Mus musculus
26 MEX3A  
Affinity Capture-RNA Homo sapiens
27 MRPS14  
Affinity Capture-MS Homo sapiens
28 FAM168A  
Affinity Capture-MS Homo sapiens
29 RAB3IL1 5866
Affinity Capture-MS Homo sapiens
30 CAPZA2 830
Affinity Capture-MS Homo sapiens
31 IFFO1  
Affinity Capture-MS Homo sapiens
32 FHL3 2275
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 LATS2 26524
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
34 CTDP1 9150
Affinity Capture-MS Homo sapiens
35 MPP5 64398
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 TNKS2 80351
Affinity Capture-Luminescence Homo sapiens
37 CDADC1  
Affinity Capture-MS Homo sapiens
38 SHMT2 6472
Affinity Capture-RNA Homo sapiens
39 WWC1  
Affinity Capture-MS Homo sapiens
40 SUZ12  
Affinity Capture-MS Homo sapiens
41 LATS1  
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
42 Myh10 77579
Affinity Capture-MS Mus musculus
43 NXF1 10482
Affinity Capture-RNA Homo sapiens
44 MRPS28  
Affinity Capture-MS Homo sapiens
45 RNF146  
Affinity Capture-Luminescence Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-Western Homo sapiens
46 WWP2 11060
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 PTCD3 55037
Affinity Capture-MS Homo sapiens
48 COG6 57511
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 ATP5J2 9551
Affinity Capture-MS Homo sapiens
50 UHRF1BP1L 23074
Affinity Capture-MS Homo sapiens
51 MOV10 4343
Affinity Capture-RNA Homo sapiens
52 WDR70 55100
Affinity Capture-MS Homo sapiens
53 MLLT4 4301
Proximity Label-MS Homo sapiens
54 YAP1 10413
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 CSNK2A1 1457
Affinity Capture-MS Homo sapiens
56 MRPS31  
Affinity Capture-MS Homo sapiens
57 FBXO2 26232
Affinity Capture-MS Homo sapiens
58 TEAD4  
Affinity Capture-MS Homo sapiens
59 RNF166  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 YWHAZ 7534
Affinity Capture-MS Homo sapiens
61 HMBOX1  
Affinity Capture-MS Homo sapiens
62 DAP3 7818
Affinity Capture-MS Homo sapiens
63 SCML1  
Affinity Capture-MS Homo sapiens
64 KRT16 3868
Affinity Capture-MS Homo sapiens
65 MRPS23 51649
Affinity Capture-MS Homo sapiens
66 MRPS24 64951
Affinity Capture-MS Homo sapiens
67 Yap1  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
68 Coro1c 23790
Affinity Capture-MS Mus musculus
69 AMOT  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
70 MAGEA4 4103
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 KRT19 3880
Proximity Label-MS Homo sapiens
72 ALOX5  
Affinity Capture-MS Homo sapiens
73 HECW2  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
74 FHL2 2274
Affinity Capture-MS Homo sapiens
75 LURAP1  
Affinity Capture-MS Homo sapiens
76 MPDZ  
Affinity Capture-MS Homo sapiens
77 DYNLL1 8655
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 NEDD4L 23327
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
79 MRPS10 55173
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which AMOTL1 is involved
PathwayEvidenceSource
Signal Transduction TAS Reactome
Signaling by Hippo TAS Reactome





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