Gene description for DDHD1
Gene name DDHD domain containing 1
Gene symbol DDHD1
Other names/aliases PA-PLA1
PAPLA1
SPG28
Species Homo sapiens
 Database cross references - DDHD1
ExoCarta ExoCarta_80821
Vesiclepedia VP_80821
Entrez Gene 80821
HGNC 19714
MIM 614603
UniProt Q8NEL9  
 DDHD1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for DDHD1
Molecular Function
    phospholipase activity GO:0004620 IBA
    protein binding GO:0005515 IPI
    phospholipase A1 activity GO:0008970 IEA
    metal ion binding GO:0046872 IEA
Biological Process
    phosphatidylinositol metabolic process GO:0046488 IEA
    positive regulation of mitochondrial fission GO:0090141 IDA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified DDHD1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for DDHD1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 WDR11 55717
Affinity Capture-MS Homo sapiens
2 PAXIP1  
Affinity Capture-MS Homo sapiens
3 COBLL1 22837
Affinity Capture-MS Homo sapiens
4 IFFO1  
Affinity Capture-MS Homo sapiens
5 RPA2 6118
Proximity Label-MS Homo sapiens
6 SCML1  
Affinity Capture-MS Homo sapiens
7 Calml3  
Affinity Capture-MS Mus musculus
8 MYO18A 399687
Affinity Capture-MS Homo sapiens
9 NR3C1 2908
Proximity Label-MS Homo sapiens
10 TYW3  
Affinity Capture-MS Homo sapiens
11 C17orf75 64149
Affinity Capture-MS Homo sapiens
12 AMOTL1 154810
Affinity Capture-MS Homo sapiens
13 PYGM 5837
Affinity Capture-MS Homo sapiens
14 AURKA 6790
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 FAM91A1 157769
Affinity Capture-MS Homo sapiens
16 PSMF1 9491
Affinity Capture-MS Homo sapiens
17 CHEK1  
Affinity Capture-MS Homo sapiens
18 MTPAP 55149
Affinity Capture-MS Homo sapiens
19 ISOC1 51015
Affinity Capture-MS Homo sapiens
20 DYNLL2 140735
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 CDADC1  
Affinity Capture-MS Homo sapiens
22 USP9Y 8287
Affinity Capture-MS Homo sapiens
23 DYNLL1 8655
Affinity Capture-MS Homo sapiens
24 MTMR6 9107
Affinity Capture-MS Homo sapiens
25 KIF2C 11004
Affinity Capture-MS Homo sapiens
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