Gene description for AURKA
Gene name aurora kinase A
Gene symbol AURKA
Other names/aliases AIK
ARK1
AURA
AURORA2
BTAK
PPP1R47
STK15
STK6
STK7
Species Homo sapiens
 Database cross references - AURKA
ExoCarta ExoCarta_6790
Vesiclepedia VP_6790
Entrez Gene 6790
HGNC 11393
MIM 603072
UniProt O14965  
 AURKA identified in exosomes derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for AURKA
Molecular Function
    ATP binding GO:0005524 IEA
    histone serine kinase activity GO:0035174 IBA
    ubiquitin protein ligase binding GO:0031625 IEA
    protein kinase binding GO:0019901 IPI
    protein kinase activity GO:0004672 IDA
    protein binding GO:0005515 IPI
    protein serine/threonine/tyrosine kinase activity GO:0004712 TAS
    protein serine/threonine kinase activity GO:0004674 EXP
Biological Process
    spindle stabilization GO:0043146 IMP
    anterior/posterior axis specification GO:0009948 IEA
    mitotic spindle organization GO:0007052 IBA
    cell division GO:0051301 IEA
    regulation of cytokinesis GO:0032465 IBA
    negative regulation of spindle checkpoint GO:0090233 IEA
    negative regulation of apoptotic process GO:0043066 IEA
    regulation of centrosome cycle GO:0046605 TAS
    regulation of protein stability GO:0031647 IMP
    positive regulation of oocyte maturation GO:1900195 IEA
    G2/M transition of mitotic cell cycle GO:0000086 TAS
    negative regulation of protein binding GO:0032091 IDA
    mitotic cell cycle GO:0000278 TAS
    protein autophosphorylation GO:0046777 TAS
    protein localization to centrosome GO:0071539 IEA
    spindle assembly involved in female meiosis I GO:0007057 IEA
    centrosome localization GO:0051642 IEA
    positive regulation of mitotic nuclear division GO:0045840 TAS
    neuron projection extension GO:1990138 IEA
    anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process GO:0031145 TAS
    mitotic nuclear division GO:0007067 TAS
    positive regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032436 IEA
    histone-serine phosphorylation GO:0035404 IEA
    protein phosphorylation GO:0006468 IDA
    mitotic centrosome separation GO:0007100 IEA
Subcellular Localization
    chromosome passenger complex GO:0032133 IBA
    germinal vesicle GO:0042585 IEA
    mitotic spindle GO:0072686 IEA
    pronucleus GO:0045120 IEA
    midbody GO:0030496 TAS
    nucleus GO:0005634 IDA
    centrosome GO:0005813 TAS
    perinuclear region of cytoplasm GO:0048471 IDA
    spindle midzone GO:0051233 IBA
    spindle microtubule GO:0005876 IDA
    microtubule cytoskeleton GO:0015630 IDA
    cytosol GO:0005829 TAS
    spindle GO:0005819 TAS
    meiotic spindle GO:0072687 IEA
    condensed nuclear chromosome, centromeric region GO:0000780 IBA
    spindle pole centrosome GO:0031616 IDA
    axon hillock GO:0043203 IEA
 Experiment description of studies that identified AURKA in exosomes
1
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for AURKA
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SOD2 6648
Affinity Capture-MS Homo sapiens
2 TACC1 6867
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
3 TPX2  
Invivo Homo sapiens
Invitro Homo sapiens
4 UBE2N 7334
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
5 CDC20 991
Affinity Capture-Western Homo sapiens
6 RAB10 10890
Affinity Capture-MS Homo sapiens
7 NME1 4830
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
8 TP53 7157
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Phenotypic Suppression Homo sapiens
9 UBE2L3 7332
Affinity Capture-MS Homo sapiens
10 NIN 51199
Biochemical Activity Homo sapiens
11 CDKN2A 1029
Affinity Capture-MS Homo sapiens
12 BRCA1 672
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
13 MBD3 53615
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
View the network image/svg+xml



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