Experiment description of studies that identified NTMT1 in sEVs

1 Experiment ID 363 MISEV standards ✘ Biophysical techniques ✔ TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1 Enriched markers ✔ DCLK1 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33991177     Organism Homo sapiens Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." Journal name Proteomics Publication year 2021 Sample Gastric cancer cells Sample name MKN1 - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 364 MISEV standards ✘ Biophysical techniques ✔ TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1 Enriched markers ✔ DCLK1 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33991177     Organism Homo sapiens Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." Journal name Proteomics Publication year 2021 Sample Gastric cancer cells Sample name MKN1 - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

3 Experiment ID 365 MISEV standards ✘ Biophysical techniques ✔ TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1 Enriched markers ✔ DCLK1 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33991177     Organism Homo sapiens Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." Journal name Proteomics Publication year 2021 Sample Gastric cancer cells Sample name MKN1 - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

4 Experiment ID 488 MISEV standards ✔ EM Biophysical techniques ✔ CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 36408942     Organism Homo sapiens Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." Journal name Proteomics Publication year 2023 Sample Mesenchymal stem cells Sample name UCMSC Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

5 Experiment ID 217 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|CD81|CD9|CD63 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23844026     Organism Homo sapiens Experiment description Characterization of human thymic exosomes. Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O." Journal name PLoS One Publication year 2013 Sample Thymus Sample name Normal-Thymus Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

Protein-protein interactions for NTMT1

Protein Interactor ExoCarta ID Identification method PubMed Species 1 VCY   Affinity Capture-MS Homo sapiens 2 DENR 8562 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 3 LAMB1 3912 Co-fractionation Homo sapiens 4 HNRNPD 3184 Two-hybrid Homo sapiens 5 CLIC1 1192 Co-fractionation Homo sapiens 6 PARP1 142 Proximity Label-MS Homo sapiens 7 DNAJB6 10049 Affinity Capture-MS Homo sapiens 8 DENND5B   Co-fractionation Homo sapiens 9 METTL13 51603 Co-fractionation Homo sapiens 10 MAPK1 5594 Co-fractionation Homo sapiens 11 CHMP4C 92421 Affinity Capture-MS Homo sapiens 12 AURKA 6790 Affinity Capture-MS Homo sapiens 13 CRYZL1 9946 Co-fractionation Homo sapiens 14 RSU1 6251 Co-fractionation Homo sapiens 15 IKBKG 8517 Reconstituted Complex Homo sapiens 16 VAMP4 8674 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 17 MAPK3 5595 Co-fractionation Homo sapiens 18 Rcc1   Affinity Capture-MS Mus musculus 19 DDB2   Biochemical Activity Homo sapiens 20 CCL5 6352 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 21 SNAP91 9892 Affinity Capture-MS Homo sapiens 22 DRG1 4733 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 23 APP 351 Reconstituted Complex Homo sapiens Reconstituted Complex Homo sapiens 24 HNRNPCL2 440563 Affinity Capture-MS Homo sapiens 25 STAU1 6780 Co-fractionation Homo sapiens 26 PPP1R16B   Affinity Capture-MS Homo sapiens 27 SMYD3 64754 Affinity Capture-MS Homo sapiens 28 VRK2 7444 Affinity Capture-MS Homo sapiens 29 TSTA3 7264 Co-fractionation Homo sapiens 30 RPA2 6118 Proximity Label-MS Homo sapiens 31 DYRK1A 1859 Affinity Capture-MS Homo sapiens 32 DPYSL2 1808 Co-fractionation Homo sapiens 33 NDRG1 10397 Co-fractionation Homo sapiens 34 DIS3 22894 Two-hybrid Homo sapiens 35 ZNF131   Affinity Capture-MS Homo sapiens 36 GLYR1 84656 Two-hybrid Homo sapiens 37 RCC1 1104 Biochemical Activity Homo sapiens 38 PLCG1 5335 Co-fractionation Homo sapiens 39 RPA3 6119 Proximity Label-MS Homo sapiens 40 TIRAP   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens View the network

Pathways in which NTMT1 is involved

No pathways found