Gene description for CCL5
Gene name chemokine (C-C motif) ligand 5
Gene symbol CCL5
Other names/aliases D17S136E
RANTES
SCYA5
SIS-delta
SISd
TCP228
eoCP
Species Homo sapiens
 Database cross references - CCL5
ExoCarta ExoCarta_6352
Vesiclepedia VP_6352
Entrez Gene 6352
HGNC 10632
MIM 187011
UniProt P13501  
 CCL5 identified in exosomes derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Plasma 34112803    
Plasma 34112803    
 Gene ontology annotations for CCL5
Molecular Function
    chemokine activity GO:0008009 NAS
    CCR1 chemokine receptor binding GO:0031726 TAS
    protein self-association GO:0043621 IDA
    chemokine receptor binding GO:0042379 IPI
    protein binding GO:0005515 IPI
    phospholipase activator activity GO:0016004 IDA
    chemokine receptor antagonist activity GO:0046817 IDA
    phosphatidylinositol phospholipase C activity GO:0004435 IDA
    CCR5 chemokine receptor binding GO:0031730 IPI
    chemoattractant activity GO:0042056 IDA
    receptor signaling protein tyrosine kinase activator activity GO:0030298 IDA
    protein homodimerization activity GO:0042803 IDA
    CCR4 chemokine receptor binding GO:0031729 TAS
    protein kinase activity GO:0004672 IDA
Biological Process
    exocytosis GO:0006887 IDA
    positive regulation of viral genome replication GO:0045070 TAS
    positive regulation of T cell proliferation GO:0042102 IDA
    cellular calcium ion homeostasis GO:0006874 IDA
    positive regulation of smooth muscle cell proliferation GO:0048661 IDA
    cellular response to fibroblast growth factor stimulus GO:0044344 IEP
    positive regulation of epithelial cell proliferation GO:0050679 IEA
    positive regulation of cell-cell adhesion mediated by integrin GO:0033634 IDA
    positive regulation of homotypic cell-cell adhesion GO:0034112 IDA
    positive regulation of T cell chemotaxis GO:0010820 IDA
    positive regulation of activation of JAK2 kinase activity GO:0010535 TAS
    positive regulation of monocyte chemotaxis GO:0090026 IDA
    cellular response to tumor necrosis factor GO:0071356 IEP
    positive regulation of cellular biosynthetic process GO:0031328 IDA
    chemotaxis GO:0006935 NAS
    leukocyte cell-cell adhesion GO:0007159 IDA
    cellular protein complex assembly GO:0043623 IDA
    calcium ion transport GO:0006816 IDA
    positive regulation of cell adhesion GO:0045785 IDA
    positive regulation of JAK-STAT cascade GO:0046427 TAS
    positive regulation of tyrosine phosphorylation of STAT protein GO:0042531 IDA
    positive regulation of protein tyrosine kinase activity GO:0061098 IDA
    cellular response to interleukin-1 GO:0071347 IEP
    macrophage chemotaxis GO:0048246 TAS
    positive regulation of T cell migration GO:2000406 IDA
    positive regulation of smooth muscle cell migration GO:0014911 IDA
    positive regulation of innate immune response GO:0045089 TAS
    positive regulation of translational initiation GO:0045948 NAS
    eosinophil chemotaxis GO:0048245 IDA
    regulation of insulin secretion GO:0050796 IDA
    negative regulation of T cell apoptotic process GO:0070233 IDA
    regulation of chronic inflammatory response GO:0002676 TAS
    protein kinase B signaling GO:0043491 IMP
    negative regulation of viral genome replication GO:0045071 IDA
    response to virus GO:0009615 TAS
    positive regulation of phosphatidylinositol 3-kinase signaling GO:0014068 IDA
    G-protein coupled receptor signaling pathway GO:0007186 IDA
    negative regulation of macrophage apoptotic process GO:2000110 IEA
    positive regulation of macrophage chemotaxis GO:0010759 IDA
    inflammatory response GO:0006954 IDA
    cellular response to organic cyclic compound GO:0071407 IDA
    protein tetramerization GO:0051262 IDA
    regulation of neuron death GO:1901214 IDA
    lipopolysaccharide-mediated signaling pathway GO:0031663 IDA
    positive regulation of phosphorylation GO:0042327 IDA
    positive chemotaxis GO:0050918 IDA
    positive regulation of natural killer cell chemotaxis GO:2000503 IDA
    regulation of T cell activation GO:0050863 IDA
    negative regulation by host of viral transcription GO:0043922 IDA
    dendritic cell chemotaxis GO:0002407 TAS
    negative regulation of chemokine-mediated signaling pathway GO:0070100 IDA
    monocyte chemotaxis GO:0002548 IC
    chemokine-mediated signaling pathway GO:0070098 TAS
    neutrophil activation GO:0042119 IDA
    activation of phospholipase D activity GO:0031584 IDA
    MAPK cascade GO:0000165 IMP
    positive regulation of calcium ion transport GO:0051928 IDA
    negative regulation of G-protein coupled receptor protein signaling pathway GO:0045744 IDA
    cell-cell signaling GO:0007267 IDA
    response to toxic substance GO:0009636 IDA
    positive regulation of T cell apoptotic process GO:0070234 IDA
    cellular response to interferon-gamma GO:0071346 IEP
    positive regulation of cell migration GO:0030335 IDA
Subcellular Localization
    extracellular region GO:0005576 TAS
    cytoplasm GO:0005737 IEA
    extracellular space GO:0005615 IEA
 Experiment description of studies that identified CCL5 in exosomes
1
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 525
MISEV standards
✔
EM
Biophysical techniques
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method ELISA
PubMed ID 34112803    
Organism Homo sapiens
Experiment description Tumor microenvironmental cytokines bound to cancer exosomes determine uptake by cytokine receptor-expressing cells and biodistribution
Authors Lima LG, Ham S, Shin H, Chai EPZ, Lek ESH, Lobb RJ, Müller AF, Mathivanan S, Yeo B, Choi Y, Parker BS, Möller A.
Journal name Nat Commun
Publication year 2021
Sample Plasma
Sample name Pooled plasma from healthy subjects
Isolation/purification methods Size exclusion chromatography
Centrifugal ultrafiltration
Flotation density -
Molecules identified in the study Protein
Methods used in the study ELISA
5
Experiment ID 526
MISEV standards
✔
EM
Biophysical techniques
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method ELISA
PubMed ID 34112803    
Organism Homo sapiens
Experiment description Tumor microenvironmental cytokines bound to cancer exosomes determine uptake by cytokine receptor-expressing cells and biodistribution
Authors Lima LG, Ham S, Shin H, Chai EPZ, Lek ESH, Lobb RJ, Müller AF, Mathivanan S, Yeo B, Choi Y, Parker BS, Möller A.
Journal name Nat Commun
Publication year 2021
Sample Plasma
Sample name Pooled plasma from breast cancer patients
Isolation/purification methods Size exclusion chromatography
Centrifugal ultrafiltration
Flotation density -
Molecules identified in the study Protein
Methods used in the study ELISA
 Protein-protein interactions for CCL5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CCR4 1233
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
2 VCAN 1462
Invitro Homo sapiens
3 CXCL8 3576
Reconstituted Complex Homo sapiens
4 CCBP2  
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
5 Igfbp7  
Reconstituted Complex Mus musculus
6 CCR1  
Invivo Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
7 CCR3  
Invivo Homo sapiens
Invivo Homo sapiens
8 CCL5 6352
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
9 GRB2 2885
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
10 FY  
Invivo Homo sapiens
Invitro Homo sapiens
11 CCR5 1234
Invivo Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
12 SDC1 6382
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
13 SDC4 6385
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which CCL5 is involved
PathwayEvidenceSource
Chemokine receptors bind chemokines TAS Reactome
G alpha (i) signalling events TAS Reactome





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