Gene description for CCL5
Gene name chemokine (C-C motif) ligand 5
Gene symbol CCL5
Other names/aliases D17S136E
RANTES
SCYA5
SIS-delta
SISd
TCP228
eoCP
Species Homo sapiens
 Database cross references - CCL5
ExoCarta ExoCarta_6352
Vesiclepedia VP_6352
Entrez Gene 6352
HGNC 10632
MIM 187011
UniProt P13501  
 CCL5 identified in exosomes derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Plasma 34112803    
Plasma 34112803    
 Gene ontology annotations for CCL5
Molecular Function
    phosphatidylinositol phospholipase C activity GO:0004435 IDA
    protein kinase activity GO:0004672 IDA
    protein binding GO:0005515 IPI
    chemokine activity GO:0008009 IBA
    chemokine activity GO:0008009 IDA
    chemokine activity GO:0008009 NAS
    phospholipase activator activity GO:0016004 IDA
    receptor signaling protein tyrosine kinase activator activity GO:0030298 IDA
    CCR1 chemokine receptor binding GO:0031726 IDA
    CCR1 chemokine receptor binding GO:0031726 IPI
    CCR1 chemokine receptor binding GO:0031726 TAS
    CCR4 chemokine receptor binding GO:0031729 TAS
    CCR5 chemokine receptor binding GO:0031730 IPI
    chemoattractant activity GO:0042056 IDA
    chemokine receptor binding GO:0042379 IPI
    identical protein binding GO:0042802 IPI
    protein homodimerization activity GO:0042803 IPI
    chemokine receptor antagonist activity GO:0046817 IDA
    CCR chemokine receptor binding GO:0048020 IBA
Biological Process
    dendritic cell chemotaxis GO:0002407 TAS
    monocyte chemotaxis GO:0002548 IC
    regulation of chronic inflammatory response GO:0002676 TAS
    calcium ion transport GO:0006816 IDA
    intracellular calcium ion homeostasis GO:0006874 IDA
    exocytosis GO:0006887 IDA
    chemotaxis GO:0006935 NAS
    inflammatory response GO:0006954 IBA
    leukocyte cell-cell adhesion GO:0007159 IDA
    G protein-coupled receptor signaling pathway GO:0007186 IDA
    cell-cell signaling GO:0007267 IDA
    response to virus GO:0009615 TAS
    response to toxic substance GO:0009636 IDA
    positive regulation of biosynthetic process GO:0009891 IDA
    positive regulation of macrophage chemotaxis GO:0010759 IDA
    positive regulation of T cell chemotaxis GO:0010820 IDA
    positive regulation of smooth muscle cell migration GO:0014911 IDA
    positive regulation of cell migration GO:0030335 IBA
    positive regulation of cell migration GO:0030335 IDA
    activation of phospholipase D activity GO:0031584 IDA
    positive regulation of cell-cell adhesion mediated by integrin GO:0033634 IDA
    positive regulation of homotypic cell-cell adhesion GO:0034112 IDA
    chemokine (C-C motif) ligand 5 signaling pathway GO:0035689 IDA
    positive regulation of T cell proliferation GO:0042102 IDA
    neutrophil activation GO:0042119 IDA
    positive regulation of phosphorylation GO:0042327 IDA
    negative regulation by host of viral transcription GO:0043922 IDA
    cellular response to fibroblast growth factor stimulus GO:0044344 IEP
    positive regulation of viral genome replication GO:0045070 TAS
    negative regulation of viral genome replication GO:0045071 IDA
    positive regulation of innate immune response GO:0045089 TAS
    negative regulation of G protein-coupled receptor signaling pathway GO:0045744 IDA
    positive regulation of G protein-coupled receptor signaling pathway GO:0045745 IDA
    positive regulation of cell adhesion GO:0045785 IDA
    positive regulation of translational initiation GO:0045948 NAS
    eosinophil chemotaxis GO:0048245 IBA
    eosinophil chemotaxis GO:0048245 IDA
    macrophage chemotaxis GO:0048246 TAS
    positive regulation of smooth muscle cell proliferation GO:0048661 IDA
    epithelial cell proliferation GO:0050673 IEA
    positive regulation of epithelial cell proliferation GO:0050679 IEA
    regulation of insulin secretion GO:0050796 IDA
    regulation of T cell activation GO:0050863 IDA
    positive chemotaxis GO:0050918 IEA
    positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051897 IDA
    positive regulation of calcium ion transport GO:0051928 IDA
    antimicrobial humoral immune response mediated by antimicrobial peptide GO:0061844 IBA
    chemokine-mediated signaling pathway GO:0070098 IBA
    chemokine-mediated signaling pathway GO:0070098 IDA
    chemokine-mediated signaling pathway GO:0070098 TAS
    negative regulation of chemokine-mediated signaling pathway GO:0070100 IEA
    negative regulation of T cell apoptotic process GO:0070233 IDA
    positive regulation of T cell apoptotic process GO:0070234 IDA
    cellular response to type II interferon GO:0071346 IEP
    cellular response to interleukin-1 GO:0071347 IEP
    cellular response to tumor necrosis factor GO:0071356 IEP
    positive regulation of monocyte chemotaxis GO:0090026 IDA
    cell surface receptor signaling pathway via STAT GO:0097696 TAS
    cellular response to virus GO:0098586 IEP
    positive regulation of receptor signaling pathway via STAT GO:1904894 IDA
    negative regulation of macrophage apoptotic process GO:2000110 IEA
    positive regulation of T cell migration GO:2000406 IDA
    positive regulation of natural killer cell chemotaxis GO:2000503 IDA
Subcellular Localization
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IC
    cytoplasm GO:0005737 IEA
 Experiment description of studies that identified CCL5 in exosomes
1
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 525
MISEV standards
✔
EM
Biophysical techniques
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method ELISA
PubMed ID 34112803    
Organism Homo sapiens
Experiment description Tumor microenvironmental cytokines bound to cancer exosomes determine uptake by cytokine receptor-expressing cells and biodistribution
Authors "Lima LG, Ham S, Shin H, Chai EPZ, Lek ESH, Lobb RJ, Müller AF, Mathivanan S, Yeo B, Choi Y, Parker BS, Möller A. "
Journal name Nat Commun
Publication year 2021
Sample Plasma
Sample name Pooled plasma from healthy subjects
Isolation/purification methods Size exclusion chromatography
Centrifugal ultrafiltration
Flotation density -
Molecules identified in the study Protein
Methods used in the study ELISA
5
Experiment ID 526
MISEV standards
✔
EM
Biophysical techniques
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method ELISA
PubMed ID 34112803    
Organism Homo sapiens
Experiment description Tumor microenvironmental cytokines bound to cancer exosomes determine uptake by cytokine receptor-expressing cells and biodistribution
Authors "Lima LG, Ham S, Shin H, Chai EPZ, Lek ESH, Lobb RJ, Müller AF, Mathivanan S, Yeo B, Choi Y, Parker BS, Möller A. "
Journal name Nat Commun
Publication year 2021
Sample Plasma
Sample name Pooled plasma from breast cancer patients
Isolation/purification methods Size exclusion chromatography
Centrifugal ultrafiltration
Flotation density -
Molecules identified in the study Protein
Methods used in the study ELISA
 Protein-protein interactions for CCL5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 VCAN 1462
Reconstituted Complex Homo sapiens
2 CCL20 6364
Reconstituted Complex Homo sapiens
3 CCL26  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
4 TRIP6 7205
Two-hybrid Homo sapiens
5 CXCL10  
Reconstituted Complex Homo sapiens
6 CXCL8 3576
Reconstituted Complex Homo sapiens
7 CCL13  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
8 AK4 205
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 CCL17  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
10 NTMT1 28989
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 CXCL2 2920
Reconstituted Complex Homo sapiens
12 SDC1 6382
Affinity Capture-Western Homo sapiens
13 RELA 5970
Two-hybrid Homo sapiens
14 SPRYD4 283377
Affinity Capture-MS Homo sapiens
15 CCL24  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
16 CCR4 1233
Reconstituted Complex Homo sapiens
17 CXCL6  
Reconstituted Complex Homo sapiens
18 CXCL12 6387
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
19 CCL11  
Reconstituted Complex Homo sapiens
20 CCL2 6347
Reconstituted Complex Homo sapiens
Phenotypic Enhancement Homo sapiens
21 CXCL14  
Reconstituted Complex Homo sapiens
22 Igfbp7  
Reconstituted Complex Mus musculus
23 CCR3  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
24 CCL27  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
25 SLC2A5 6518
Two-hybrid Homo sapiens
26 GPD1L 23171
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 SPATA20  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 S100A13 6284
Affinity Capture-MS Homo sapiens
29 C4A 720
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 EDC4 23644
Two-hybrid Homo sapiens
31 CCL25  
Reconstituted Complex Homo sapiens
32 CXCL17  
Reconstituted Complex Homo sapiens
33 XCL1  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
34 CCR5 1234
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
35 XCL2  
Reconstituted Complex Homo sapiens
36 ACKR2  
Reconstituted Complex Homo sapiens
37 SDC4 6385
Affinity Capture-Western Homo sapiens
38 CCL21  
Reconstituted Complex Homo sapiens
39 CXCL9  
Reconstituted Complex Homo sapiens
40 CXCL11  
Reconstituted Complex Homo sapiens
41 CCL28 56477
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
42 CCR1  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
43 UBAC1 10422
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 PPBP 5473
Reconstituted Complex Homo sapiens
45 RANGAP1 5905
Two-hybrid Homo sapiens
46 CCL16  
Reconstituted Complex Homo sapiens
47 PF4 5196
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
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