Gene description for CXCL12
Gene name chemokine (C-X-C motif) ligand 12
Gene symbol CXCL12
Other names/aliases IRH
PBSF
SCYB12
SDF1
TLSF
TPAR1
Species Homo sapiens
 Database cross references - CXCL12
ExoCarta ExoCarta_6387
Vesiclepedia VP_6387
Entrez Gene 6387
HGNC 10672
MIM 600835
UniProt P48061  
 CXCL12 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
 Gene ontology annotations for CXCL12
Molecular Function
    signaling receptor binding GO:0005102 TAS
    integrin binding GO:0005178 IDA
    protein binding GO:0005515 IPI
    chemokine activity GO:0008009 IBA
    chemokine activity GO:0008009 IDA
    chemokine activity GO:0008009 IMP
    chemokine activity GO:0008009 TAS
    growth factor activity GO:0008083 IEA
    chemokine receptor binding GO:0042379 IMP
    CXCR chemokine receptor binding GO:0045236 IBA
    CXCR chemokine receptor binding GO:0045236 IDA
Biological Process
    response to hypoxia GO:0001666 IEA
    neuron migration GO:0001764 IEA
    positive regulation of endothelial cell proliferation GO:0001938 IEA
    intracellular calcium ion homeostasis GO:0006874 TAS
    chemotaxis GO:0006935 TAS
    defense response GO:0006952 IEA
    immune response GO:0006955 IEA
    cell adhesion GO:0007155 TAS
    signal transduction GO:0007165 TAS
    G protein-coupled receptor signaling pathway GO:0007186 TAS
    axon guidance GO:0007411 IBA
    blood circulation GO:0008015 TAS
    regulation of actin polymerization or depolymerization GO:0008064 TAS
    adult locomotory behavior GO:0008344 IEA
    response to virus GO:0009615 TAS
    T cell chemotaxis GO:0010818 IDA
    telencephalon cell migration GO:0022029 IEA
    positive regulation of cell migration GO:0030335 IBA
    animal organ regeneration GO:0031100 IEA
    killing of cells of another organism GO:0031640 IDA
    positive regulation of dopamine secretion GO:0033603 IEA
    integrin activation GO:0033622 IDA
    chemokine (C-X-C motif) ligand 12 signaling pathway GO:0038146 IMP
    CXCL12-activated CXCR4 signaling pathway GO:0038160 IGI
    response to peptide hormone GO:0043434 IEA
    positive regulation of neuron differentiation GO:0045666 IEA
    positive regulation of cell adhesion GO:0045785 IDA
    positive regulation of axon extension involved in axon guidance GO:0048842 IEA
    induction of positive chemotaxis GO:0050930 IBA
    detection of temperature stimulus involved in sensory perception of pain GO:0050965 IEA
    detection of mechanical stimulus involved in sensory perception of pain GO:0050966 IEA
    cell chemotaxis GO:0060326 IBA
    cell chemotaxis GO:0060326 IDA
    antimicrobial humoral immune response mediated by antimicrobial peptide GO:0061844 IDA
    chemokine-mediated signaling pathway GO:0070098 IBA
    chemokine-mediated signaling pathway GO:0070098 IDA
    positive regulation of monocyte chemotaxis GO:0090026 IDA
    positive regulation of calcium ion import GO:0090280 TAS
    negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage GO:1902230 IDA
    negative regulation of leukocyte tethering or rolling GO:1903237 IDA
    positive regulation of vasculature development GO:1904018 IMP
    response to ultrasound GO:1990478 IEA
    cellular response to chemokine GO:1990869 IMP
    positive regulation of T cell migration GO:2000406 IDA
    negative regulation of dendritic cell apoptotic process GO:2000669 IDA
Subcellular Localization
    extracellular region GO:0005576 TAS
    external side of plasma membrane GO:0009897 IBA
    collagen-containing extracellular matrix GO:0062023 HDA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified CXCL12 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for CXCL12
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 VCAN 1462
Reconstituted Complex Homo sapiens
2 CCL20 6364
Reconstituted Complex Homo sapiens
3 CCL26  
Reconstituted Complex Homo sapiens
4 CXCL10  
Reconstituted Complex Homo sapiens
5 CCL13  
Reconstituted Complex Homo sapiens
6 CXCR4 7852
Co-crystal Structure Homo sapiens
7 CXCL2 2920
Reconstituted Complex Homo sapiens
8 CXCL6  
Reconstituted Complex Homo sapiens
9 CXCL12 6387
Co-crystal Structure Homo sapiens
Co-crystal Structure Homo sapiens
10 CCL11  
Reconstituted Complex Homo sapiens
11 CXCL14  
Reconstituted Complex Homo sapiens
12 PF4V1 5197
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
13 CCL5 6352
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
14 FN1 2335
Reconstituted Complex Homo sapiens
15 DAZAP2  
Two-hybrid Homo sapiens
16 CCL25  
Reconstituted Complex Homo sapiens
17 CXCL17  
Reconstituted Complex Homo sapiens
18 XCL1  
Reconstituted Complex Homo sapiens
19 XCL2  
Reconstituted Complex Homo sapiens
20 CCL21  
Reconstituted Complex Homo sapiens
21 CXCL9  
Reconstituted Complex Homo sapiens
22 CXCL11  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
23 CCL28 56477
Reconstituted Complex Homo sapiens
24 PPBP 5473
Reconstituted Complex Homo sapiens
25 PF4 5196
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
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