Gene description for PF4
Gene name platelet factor 4
Gene symbol PF4
Other names/aliases CXCL4
PF-4
SCYB4
Species Homo sapiens
 Database cross references - PF4
ExoCarta ExoCarta_5196
Vesiclepedia VP_5196
Entrez Gene 5196
HGNC 8861
MIM 173460
UniProt P02776  
 PF4 identified in exosomes derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
 Gene ontology annotations for PF4
Molecular Function
    CXCR3 chemokine receptor binding GO:0048248 IDA
    chemokine activity GO:0008009 TAS
    heparin binding GO:0008201 IDA
Biological Process
    response to lipopolysaccharide GO:0032496 IBA
    positive regulation of gene expression GO:0010628 IDA
    G-protein coupled receptor signaling pathway GO:0007186 IBA
    positive regulation of cAMP-mediated signaling GO:0043950 IDA
    immune response GO:0006955 IBA
    chemokine-mediated signaling pathway GO:0070098 IBA
    blood coagulation GO:0007596 TAS
    regulation of cell proliferation GO:0042127 IDA
    positive regulation of cAMP metabolic process GO:0030816 IDA
    leukocyte chemotaxis GO:0030595 IDA
    positive regulation of macrophage differentiation GO:0045651 IDA
    negative regulation of angiogenesis GO:0016525 IDA
    positive regulation of macrophage derived foam cell differentiation GO:0010744 IDA
    platelet degranulation GO:0002576 TAS
    negative regulation of cytolysis GO:0045918 IDA
    inflammatory response GO:0006954 IBA
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IDA
    positive regulation of tumor necrosis factor production GO:0032760 IDA
    cytokine-mediated signaling pathway GO:0019221 IDA
    negative regulation of megakaryocyte differentiation GO:0045653 IDA
    platelet activation GO:0030168 TAS
    negative regulation of extrinsic apoptotic signaling pathway in absence of ligand GO:2001240 IDA
    positive regulation of leukocyte chemotaxis GO:0002690 IBA
    negative regulation of MHC class II biosynthetic process GO:0045347 IDA
Subcellular Localization
    platelet alpha granule lumen GO:0031093 TAS
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 IBA
 Experiment description of studies that identified PF4 in exosomes
1
Experiment ID 497
MISEV standards
✘ EV Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD.
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 498
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD.
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for PF4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SDC2 6383
Invitro Homo sapiens
2 THBD 7056
Reconstituted Complex Homo sapiens
3 LDLR 3949
Reconstituted Complex Homo sapiens
4 CXCR3  
Reconstituted Complex Homo sapiens
5 PROC 5624
Reconstituted Complex Homo sapiens
6 SRGN 5552
Invivo Homo sapiens
Invitro Homo sapiens
7 FGF2 2247
Invivo Homo sapiens
Invitro Homo sapiens
8 PF4 5196
Invitro Homo sapiens
Co-crystal Structure Homo sapiens
Co-crystal Structure Homo sapiens
Invitro Homo sapiens
Co-crystal Structure Homo sapiens
Co-crystal Structure Homo sapiens
View the network image/svg+xml



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