Gene description for PF4
Gene name platelet factor 4
Gene symbol PF4
Other names/aliases CXCL4
PF-4
SCYB4
Species Homo sapiens
 Database cross references - PF4
ExoCarta ExoCarta_5196
Vesiclepedia VP_5196
Entrez Gene 5196
HGNC 8861
MIM 173460
UniProt P02776  
 PF4 identified in exosomes derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
 Gene ontology annotations for PF4
Molecular Function
    protein binding GO:0005515 IPI
    chemokine activity GO:0008009 IBA
    chemokine activity GO:0008009 IDA
    chemokine activity GO:0008009 TAS
    heparin binding GO:0008201 IDA
    CXCR chemokine receptor binding GO:0045236 IBA
    receptor ligand activity GO:0048018 IDA
    CXCR3 chemokine receptor binding GO:0048248 IDA
Biological Process
    monocyte chemotaxis GO:0002548 IDA
    inflammatory response GO:0006954 IBA
    adenylate cyclase-activating G protein-coupled receptor signaling pathway GO:0007189 IDA
    positive regulation of gene expression GO:0010628 IDA
    positive regulation of macrophage derived foam cell differentiation GO:0010744 IDA
    negative regulation of angiogenesis GO:0016525 IDA
    cytokine-mediated signaling pathway GO:0019221 IDA
    platelet activation GO:0030168 IDA
    negative regulation of blood coagulation GO:0030195 IDA
    neutrophil chemotaxis GO:0030593 IBA
    leukocyte chemotaxis GO:0030595 IDA
    killing of cells of another organism GO:0031640 IDA
    positive regulation of tumor necrosis factor production GO:0032760 IDA
    regulation of cell population proliferation GO:0042127 IDA
    defense response to protozoan GO:0042832 IDA
    negative regulation of MHC class II biosynthetic process GO:0045347 IDA
    positive regulation of macrophage differentiation GO:0045651 IDA
    negative regulation of megakaryocyte differentiation GO:0045653 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    killing by host of symbiont cells GO:0051873 IDA
    antimicrobial humoral immune response mediated by antimicrobial peptide GO:0061844 IBA
    antimicrobial humoral immune response mediated by antimicrobial peptide GO:0061844 IDA
    cellular response to lipopolysaccharide GO:0071222 IBA
    negative regulation of extrinsic apoptotic signaling pathway in absence of ligand GO:2001240 IDA
Subcellular Localization
    extracellular region GO:0005576 NAS
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IDA
    cytoplasm GO:0005737 IDA
    platelet alpha granule lumen GO:0031093 TAS
    collagen-containing extracellular matrix GO:0062023 HDA
 Experiment description of studies that identified PF4 in exosomes
1
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for PF4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 VCAN 1462
Reconstituted Complex Homo sapiens
2 CCL20 6364
Reconstituted Complex Homo sapiens
3 CXCR3  
Reconstituted Complex Homo sapiens
4 CXCL10  
Reconstituted Complex Homo sapiens
5 CXCL8 3576
Reconstituted Complex Homo sapiens
6 CCL13  
Reconstituted Complex Homo sapiens
7 CXCL2 2920
Reconstituted Complex Homo sapiens
8 CCL26  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
9 THBD 7056
Reconstituted Complex Homo sapiens
10 CXCL6  
Reconstituted Complex Homo sapiens
11 CXCL12 6387
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
12 PROC 5624
Reconstituted Complex Homo sapiens
13 BLZF1  
Affinity Capture-MS Homo sapiens
14 CCL11  
Reconstituted Complex Homo sapiens
15 CCL2 6347
Reconstituted Complex Homo sapiens
16 CXCL14  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
17 CCL5 6352
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
18 CCL25  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
19 CXCL17  
Reconstituted Complex Homo sapiens
20 XCL1  
Reconstituted Complex Homo sapiens
21 XCL2  
Reconstituted Complex Homo sapiens
22 CCL21  
Reconstituted Complex Homo sapiens
23 LDLR 3949
Reconstituted Complex Homo sapiens
24 MOCS1  
Affinity Capture-MS Homo sapiens
25 CXCL9  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
26 CXCL11  
Reconstituted Complex Homo sapiens
27 CCL28 56477
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
28 PF4 5196
Co-crystal Structure Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
View the network image/svg+xml



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