Gene description for CCL28
Gene name chemokine (C-C motif) ligand 28
Gene symbol CCL28
Other names/aliases CCK1
MEC
SCYA28
Species Homo sapiens
 Database cross references - CCL28
ExoCarta ExoCarta_56477
Vesiclepedia VP_56477
Entrez Gene 56477
HGNC 17700
MIM 605240
UniProt Q9NRJ3  
 CCL28 identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells Unpublished / Not applicable
Saliva 19199708    
 Gene ontology annotations for CCL28
Molecular Function
    protein binding GO:0005515 IPI
    chemokine activity GO:0008009 IBA
    chemokine activity GO:0008009 IDA
    chemokine activity GO:0008009 TAS
    CXCR chemokine receptor binding GO:0045236 IBA
Biological Process
    positive regulation of cell-matrix adhesion GO:0001954 IBA
    positive regulation of cell-matrix adhesion GO:0001954 IDA
    chemotaxis GO:0006935 TAS
    inflammatory response GO:0006954 IBA
    immune response GO:0006955 IEA
    signal transduction GO:0007165 IEA
    positive regulation of cytosolic calcium ion concentration GO:0007204 IEA
    response to nutrient GO:0007584 IEA
    neutrophil chemotaxis GO:0030593 IBA
    killing of cells of another organism GO:0031640 IMP
    cytolysis in another organism GO:0051715 IMP
    cell chemotaxis GO:0060326 IDA
    antimicrobial humoral immune response mediated by antimicrobial peptide GO:0061844 IBA
    antimicrobial humoral immune response mediated by antimicrobial peptide GO:0061844 IMP
    cellular response to lipopolysaccharide GO:0071222 IBA
    negative regulation of leukocyte tethering or rolling GO:1903237 IDA
Subcellular Localization
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IDA
    secretory granule GO:0030141 IDA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified CCL28 in exosomes
1
Experiment ID 126
MISEV standards
Biophysical techniques
GAPDH
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Antibody array
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
2
Experiment ID 66
MISEV standards
IEM
Biophysical techniques
TSG101|Alix|CD63|CD81
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR"
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for CCL28
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 STUB1 10273
Two-hybrid Homo sapiens
2 CXCL2 2920
Reconstituted Complex Homo sapiens
3 CCL26  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
4 CXCL10  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
5 CCL13  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
6 PSMA3 5684
Two-hybrid Homo sapiens
7 CCL17  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
8 CXCL12 6387
Reconstituted Complex Homo sapiens
9 CCL11  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
10 CXCL14  
Reconstituted Complex Homo sapiens
11 PF4V1 5197
Reconstituted Complex Homo sapiens
12 CXCL13  
Reconstituted Complex Homo sapiens
13 TSC1 7248
Two-hybrid Homo sapiens
14 CCL5 6352
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
15 CXCL17  
Reconstituted Complex Homo sapiens
16 XCL1  
Reconstituted Complex Homo sapiens
17 XCL2  
Reconstituted Complex Homo sapiens
18 CCL21  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
19 CXCL9  
Reconstituted Complex Homo sapiens
20 PPBP 5473
Reconstituted Complex Homo sapiens
21 DDI1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
22 PF4 5196
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
23 DYNLT3 6990
Two-hybrid Homo sapiens
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