Gene description for CCL20
Gene name chemokine (C-C motif) ligand 20
Gene symbol CCL20
Other names/aliases CKb4
Exodus
LARC
MIP-3-alpha
MIP-3a
MIP3A
SCYA20
ST38
Species Homo sapiens
 Database cross references - CCL20
ExoCarta ExoCarta_6364
Vesiclepedia VP_6364
Entrez Gene 6364
HGNC 10619
MIM 601960
UniProt P78556  
 CCL20 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
Mesenchymal stem cells Unpublished / Not applicable
 Gene ontology annotations for CCL20
Molecular Function
    protein binding GO:0005515 IPI
    CCR6 chemokine receptor binding GO:0031731 IDA
Biological Process
    chemotaxis GO:0006935 IDA
    immune response GO:0006955 TAS
    signal transduction GO:0007165 TAS
    cell-cell signaling GO:0007267 TAS
    calcium-mediated signaling GO:0019722 IDA
    killing of cells of another organism GO:0031640 IDA
    defense response to bacterium GO:0042742 IEA
    cell chemotaxis GO:0060326 IDA
    antimicrobial humoral immune response mediated by antimicrobial peptide GO:0061844 IDA
    T cell migration GO:0072678 ISS
    thymocyte migration GO:0072679 ISS
    positive regulation of T cell migration GO:2000406 IDA
Subcellular Localization
    extracellular region GO:0005576 TAS
 Experiment description of studies that identified CCL20 in exosomes
1
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
2
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
3
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Antibody array
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
 Protein-protein interactions for CCL20
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 VCAN 1462
Reconstituted Complex Homo sapiens
2 CXCL12 6387
Reconstituted Complex Homo sapiens
3 CCR6  
Reconstituted Complex Homo sapiens
4 CXCL11  
Reconstituted Complex Homo sapiens
5 CXCL17  
Reconstituted Complex Homo sapiens
6 CCL5 6352
Reconstituted Complex Homo sapiens
7 TGFB3 7043
Two-hybrid Homo sapiens
8 XCL2  
Reconstituted Complex Homo sapiens
9 PF4 5196
Reconstituted Complex Homo sapiens
10 CXCL5  
Reconstituted Complex Homo sapiens
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