Gene description for VCAN
Gene name versican
Gene symbol VCAN
Other names/aliases CSPG2
ERVR
GHAP
PG-M
WGN
WGN1
Species Homo sapiens
 Database cross references - VCAN
ExoCarta ExoCarta_1462
Entrez Gene 1462
HGNC 2464
MIM 118661
UniProt P13611  
 VCAN identified in exosomes derived from the following tissue/cell type
Melanoma cells 25950383    
Mesenchymal stem cells Unpublished / Not applicable
 Gene ontology annotations for VCAN
Molecular Function
    calcium ion binding GO:0005509 IEA
    hyaluronic acid binding GO:0005540 IEA
    protein binding GO:0005515 IPI
    carbohydrate binding GO:0030246 IEA
    glycosaminoglycan binding GO:0005539 TAS
Biological Process
    osteoblast differentiation GO:0001649 IDA
    glycosaminoglycan metabolic process GO:0030203 TAS
    cell adhesion GO:0007155 IEA
    dermatan sulfate biosynthetic process GO:0030208 TAS
    chondroitin sulfate biosynthetic process GO:0030206 TAS
    chondroitin sulfate metabolic process GO:0030204 TAS
    skeletal system development GO:0001501 IBA
    cell recognition GO:0008037 TAS
    heart development GO:0007507 IEA
    central nervous system development GO:0007417 IBA
    multicellular organismal development GO:0007275 TAS
    small molecule metabolic process GO:0044281 TAS
    carbohydrate metabolic process GO:0005975 TAS
    chondroitin sulfate catabolic process GO:0030207 TAS
    glial cell migration GO:0008347 IDA
    extracellular matrix organization GO:0030198 TAS
Subcellular Localization
    extracellular space GO:0005615 IDA
    membrane GO:0016020 IDA
    intracellular membrane-bounded organelle GO:0043231 IDA
    proteinaceous extracellular matrix GO:0005578 IEA
    Golgi lumen GO:0005796 TAS
    extracellular region GO:0005576 TAS
    lysosomal lumen GO:0043202 TAS
    extracellular matrix GO:0031012 ISS
 Experiment description of studies that identified VCAN in exosomes
1
Experiment ID 258
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
FLOT1|CD81
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name SKMEL28
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 126
ISEV standards
EV Biophysical techniques
GAPDH
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
 Protein-protein interactions for VCAN
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ITGB1 3688
Invivo Homo sapiens
2 CCL20 6364
Invitro Homo sapiens
3 CCL8  
Invitro Homo sapiens
4 PLA2G2A 5320
Invitro Homo sapiens
5 FBN1 2200
Reconstituted Complex Homo sapiens
6 CCL5  
Invitro Homo sapiens
7 CD44 960
Invitro Homo sapiens
8 FBLN2 2199
Reconstituted Complex Homo sapiens
9 HAPLN1 1404
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
10 SELP 6403
Invitro Homo sapiens
11 CCL2 6347
Invitro Homo sapiens
12 AGC1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
13 CXCL10  
Invitro Homo sapiens
14 FBLN1 2192
Invitro Homo sapiens
15 XCL1  
Invitro Homo sapiens
16 ITGA4 3676
Invitro Homo sapiens
17 CCL21  
Invitro Homo sapiens
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