Gene description for SELP
Gene name selectin P (granule membrane protein 140kDa, antigen CD62)
Gene symbol SELP
Other names/aliases CD62
CD62P
GMP140
GRMP
LECAM3
PADGEM
PSEL
Species Homo sapiens
 Database cross references - SELP
ExoCarta ExoCarta_6403
Entrez Gene 6403
HGNC 10721
MIM 173610
UniProt P16109  
 SELP identified in exosomes derived from the following tissue/cell type
Platelets 10572093    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for SELP
Molecular Function
    calcium-dependent protein binding GO:0048306 IEA
    sialic acid binding GO:0033691 IDA
    fucose binding GO:0042806 IDA
    protein binding GO:0005515 IPI
    oligosaccharide binding GO:0070492 IDA
    heparin binding GO:0008201 IDA
    lipopolysaccharide binding GO:0001530 IMP
    glycoprotein binding GO:0001948 IEA
    glycosphingolipid binding GO:0043208 TAS
Biological Process
    leukocyte tethering or rolling GO:0050901 IEA
    leukocyte cell-cell adhesion GO:0007159 IDA
    inflammatory response GO:0006954 IEA
    heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules GO:0007157 IEA
    cell adhesion GO:0007155 TAS
    regulation of integrin activation GO:0033623 IMP
    blood coagulation GO:0007596 TAS
    platelet activation GO:0030168 TAS
    platelet degranulation GO:0002576 TAS
    positive regulation of leukocyte migration GO:0002687 IEA
    positive regulation of phosphatidylinositol 3-kinase signaling GO:0014068 IDA
    response to lipopolysaccharide GO:0032496 IC
    positive regulation of platelet activation GO:0010572 ISS
    defense response to Gram-negative bacterium GO:0050829 IC
    leukocyte migration GO:0050900 TAS
Subcellular Localization
    integral component of plasma membrane GO:0005887 TAS
    plasma membrane GO:0005886 TAS
    extracellular space GO:0005615 IDA
    platelet alpha granule membrane GO:0031092 TAS
    external side of plasma membrane GO:0009897 IDA
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IDA
    platelet dense granule membrane GO:0031088 TAS
 Experiment description of studies that identified SELP in exosomes
1
Experiment ID 31
ISEV standards
✔
EM|IEM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD63
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Immunoelectron Microscopy
PubMed ID 10572093    
Organism Homo sapiens
Experiment description Activated platelets release two types of membrane vesicles: microvesicles by surface shedding and exosomes derived from exocytosis of multivesicular bodies and alpha-granules.
Authors Heijnen HF, Schiel AE, Fijnheer R, Geuze HJ, Sixma JJ
Journal name BLOOD
Publication year 1999
Sample Platelets
Sample name Platelets
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
FACS
Immunoelectron Microscopy
2
Experiment ID 231
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 232
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 233
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SELP
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 VCAN 1462
Invitro Homo sapiens
2 GP1BA 2811
Invivo Homo sapiens
Invitro Homo sapiens
3 EZR 7430
Invivo Homo sapiens
4 SELPLG 6404
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
5 AP1M1 8907
Invitro Homo sapiens
6 SERPING1 710
Affinity Capture-Western Homo sapiens
7 SNX17 9784
Two-hybrid Homo sapiens
8 CD24  
Invivo Homo sapiens
9 FUT4  
Invitro Homo sapiens
View the network image/svg+xml
 Pathways in which SELP is involved
PathwayEvidenceSource
Cell surface interactions at the vascular wall TAS Reactome
Platelet degranulation TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here