Gene description for SELP
Gene name selectin P (granule membrane protein 140kDa, antigen CD62)
Gene symbol SELP
Other names/aliases CD62
CD62P
GMP140
GRMP
LECAM3
PADGEM
PSEL
Species Homo sapiens
 Database cross references - SELP
ExoCarta ExoCarta_6403
Vesiclepedia VP_6403
Entrez Gene 6403
HGNC 10721
MIM 173610
UniProt P16109  
 SELP identified in exosomes derived from the following tissue/cell type
Platelets 10572093    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for SELP
Molecular Function
    lipopolysaccharide binding GO:0001530 IMP
    integrin binding GO:0005178 IDA
    calcium ion binding GO:0005509 IDA
    protein binding GO:0005515 IPI
    heparin binding GO:0008201 IDA
    sialic acid binding GO:0033691 IBA
    sialic acid binding GO:0033691 IDA
    fucose binding GO:0042806 IDA
    glycosphingolipid binding GO:0043208 TAS
    calcium-dependent protein binding GO:0048306 IEA
    oligosaccharide binding GO:0070492 IBA
    oligosaccharide binding GO:0070492 IDA
Biological Process
    positive regulation of leukocyte migration GO:0002687 IEA
    inflammatory response GO:0006954 IEA
    cell adhesion GO:0007155 TAS
    heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules GO:0007157 IBA
    leukocyte cell-cell adhesion GO:0007159 IDA
    positive regulation of platelet activation GO:0010572 ISS
    calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules GO:0016339 IMP
    response to lipopolysaccharide GO:0032496 IC
    regulation of integrin activation GO:0033623 IMP
    response to cytokine GO:0034097 IBA
    defense response to Gram-negative bacterium GO:0050829 IC
    leukocyte tethering or rolling GO:0050901 IBA
    leukocyte tethering or rolling GO:0050901 IMP
    positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051897 IDA
    cell-cell adhesion GO:0098609 IDA
    positive regulation of leukocyte tethering or rolling GO:1903238 ISS
Subcellular Localization
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IDA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    external side of plasma membrane GO:0009897 IBA
    external side of plasma membrane GO:0009897 IDA
    platelet dense granule membrane GO:0031088 TAS
    platelet alpha granule membrane GO:0031092 IDA
    platelet alpha granule membrane GO:0031092 TAS
 Experiment description of studies that identified SELP in exosomes
1
Experiment ID 31
MISEV standards
EM|IEM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Immunoelectron Microscopy
PubMed ID 10572093    
Organism Homo sapiens
Experiment description Activated platelets release two types of membrane vesicles: microvesicles by surface shedding and exosomes derived from exocytosis of multivesicular bodies and alpha-granules.
Authors "Heijnen HF, Schiel AE, Fijnheer R, Geuze HJ, Sixma JJ"
Journal name BLOOD
Publication year 1999
Sample Platelets
Sample name Platelets
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
FACS
Immunoelectron Microscopy
2
Experiment ID 231
MISEV standards
Biophysical techniques
Alix|CD63|CD9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 232
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 233
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SELP
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 VCAN 1462
Reconstituted Complex Homo sapiens
2 GP1BA 2811
Reconstituted Complex Homo sapiens
3 EZR 7430
Reconstituted Complex Homo sapiens
4 SNX27 81609
Reconstituted Complex Homo sapiens
5 SELPLG 6404
Affinity Capture-Western Homo sapiens
6 AP1M1 8907
Co-crystal Structure Homo sapiens
7 SERPING1 710
Affinity Capture-Western Homo sapiens
8 CD24 100133941
Reconstituted Complex Homo sapiens
9 SNX17 9784
Two-hybrid Homo sapiens
Co-crystal Structure Homo sapiens
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
View the network image/svg+xml



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