Gene description for CD24
Gene name CD24 molecule
Gene symbol CD24
Other names/aliases CD24A
Species Homo sapiens
 Database cross references - CD24
ExoCarta ExoCarta_100133941
Vesiclepedia VP_100133941
Entrez Gene 100133941
HGNC 1645
MIM 600074
UniProt P25063  
 CD24 identified in exosomes derived from the following tissue/cell type
Amniotic fluid 17700640    
Malignant ascites 21601258    
Malignant ascites 19188015    
Ovarian cancer cells 19188015    
Ovarian cancer cells 19188015    
Pleural effusions 21601258    
Serum 21601258    
Urine 17700640    
 Gene ontology annotations for CD24
Molecular Function
    protein binding GO:0005515 IPI
    protein kinase binding GO:0019901 IPI
    protein tyrosine kinase activator activity GO:0030296 IBA
    protein tyrosine kinase activator activity GO:0030296 IDA
Biological Process
    response to hypoxia GO:0001666 IEP
    cell activation GO:0001775 IBA
    cell activation GO:0001775 IDA
    regulation of cytokine-mediated signaling pathway GO:0001959 ISS
    response to molecule of bacterial origin GO:0002237 ISS
    immune response-regulating cell surface receptor signaling pathway GO:0002768 IC
    positive regulation of cytosolic calcium ion concentration GO:0007204 IDA
    Wnt signaling pathway GO:0016055 NAS
    cell migration GO:0016477 ISS
    regulation of cell-cell adhesion GO:0022407 IBA
    regulation of epithelial cell differentiation GO:0030856 NAS
    T cell costimulation GO:0031295 IDA
    B cell receptor transport into membrane raft GO:0032597 IDA
    chemokine receptor transport out of membrane raft GO:0032600 ISS
    negative regulation of transforming growth factor beta3 production GO:0032913 IMP
    positive regulation of activated T cell proliferation GO:0042104 IDA
    regulation of phosphorylation GO:0042325 IDA
    cholesterol homeostasis GO:0042632 ISS
    positive regulation of MAP kinase activity GO:0043406 IDA
    regulation of MAPK cascade GO:0043408 IDA
    response to estrogen GO:0043627 IEP
    respiratory burst GO:0045730 IDA
    podocyte differentiation GO:0072112 IMP
    glomerular parietal epithelial cell differentiation GO:0072139 IMP
    intrinsic apoptotic signaling pathway GO:0097193 NAS
    cell-cell adhesion GO:0098609 NAS
    positive regulation of nephron tubule epithelial cell differentiation GO:2000768 IMP
Subcellular Localization
    external side of plasma membrane GO:0009897 IBA
    cell surface GO:0009986 IDA
    membrane GO:0016020 IDA
    intracellular membrane-bounded organelle GO:0043231 IDA
    membrane raft GO:0045121 IBA
    membrane raft GO:0045121 IDA
 Experiment description of studies that identified CD24 in exosomes
1
Experiment ID 58
MISEV standards
✘ Biophysical techniques
✔
HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 17700640    
Organism Homo sapiens
Experiment description CD24 is a marker of exosomes secreted into urine and amniotic fluid.
Authors "Keller S, Rupp C, Stoeck A, Runz S, Fogel M, Lugert S, Hager HD, Abdel-Bakky MS, Gutwein P, Altevogt P"
Journal name KI
Publication year 2007
Sample Amniotic fluid
Sample name Amniotic fluid
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.08-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
2
Experiment ID 108
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
HSP70|CD81|CD9|EpCAM
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
Immunoelctron microscopy
PubMed ID 21601258    
Organism Homo sapiens
Experiment description Loss of EpCAM expression in breast cancer derived serum exosomes: Role of proteolytic cleavage.
Authors "Rupp AK, Rupp C, Keller S, Brase JC, Ehehalt R, Fogel M, Moldenhauer G, Marme F, Sultmann H, Altevogt P."
Journal name GO
Publication year 2011
Sample Malignant ascites
Sample name Malignant ascites - Ovarian cancer
Isolation/purification methods "Differential centrifugation
Sucrose density gradient
Immunobeads (EPCAM, CD24)"
Flotation density 1.04-1.15 g/mL
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Immunoelctron microscopy
3
Experiment ID 131
MISEV standards
✘ Biophysical techniques
✔
HSP70|EpCAM|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 19188015    
Organism Homo sapiens
Experiment description Systemic presence and tumor-growth promoting effect of ovarian carcinoma released exosomes
Authors "Keller S, Konig AK, Marme F, Runz S, Wolterink S, Koensgen D, Mustea A, Sehouli J, Altevogt P."
Journal name CL
Publication year 2009
Sample Malignant ascites
Sample name Malignant ascites - Ovarian cancer
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.08-1.14 mg/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Cytofluorographic analysis
Annexin-V-FITC
Western blotting
FACS
4
Experiment ID 132
MISEV standards
✘ Biophysical techniques
✔
CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method FACS
PubMed ID 19188015    
Organism Homo sapiens
Experiment description Systemic presence and tumor-growth promoting effect of ovarian carcinoma released exosomes
Authors "Keller S, Konig AK, Marme F, Runz S, Wolterink S, Koensgen D, Mustea A, Sehouli J, Altevogt P."
Journal name CL
Publication year 2009
Sample Ovarian cancer cells
Sample name OVMz
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.08-1.14 mg/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Cytofluorographic analysis
Annexin-V-FITC
FACS
5
Experiment ID 133
MISEV standards
✘ Biophysical techniques
✔
CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method FACS
PubMed ID 19188015    
Organism Homo sapiens
Experiment description Systemic presence and tumor-growth promoting effect of ovarian carcinoma released exosomes
Authors "Keller S, Konig AK, Marme F, Runz S, Wolterink S, Koensgen D, Mustea A, Sehouli J, Altevogt P."
Journal name CL
Publication year 2009
Sample Ovarian cancer cells
Sample name SKOV3ip
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.08-1.14 mg/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Cytofluorographic analysis
Annexin-V-FITC
FACS
6
Experiment ID 109
MISEV standards
✘ Biophysical techniques
✔
HSP70|CD9|MHCII|HLA-DR|CD24|EpCAM
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 21601258    
Organism Homo sapiens
Experiment description Loss of EpCAM expression in breast cancer derived serum exosomes: Role of proteolytic cleavage.
Authors "Rupp AK, Rupp C, Keller S, Brase JC, Ehehalt R, Fogel M, Moldenhauer G, Marme F, Sultmann H, Altevogt P."
Journal name GO
Publication year 2011
Sample Pleural effusions
Sample name Pleural effusions - Breast cancer
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (EPCAM)
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
7
Experiment ID 111
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD24|EpCAM
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 21601258    
Organism Homo sapiens
Experiment description Loss of EpCAM expression in breast cancer derived serum exosomes: Role of proteolytic cleavage.
Authors "Rupp AK, Rupp C, Keller S, Brase JC, Ehehalt R, Fogel M, Moldenhauer G, Marme F, Sultmann H, Altevogt P."
Journal name GO
Publication year 2011
Sample Serum
Sample name Serum - Breast cancer
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (CD24)
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
8
Experiment ID 57
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
FACS
PubMed ID 17700640    
Organism Homo sapiens
Experiment description CD24 is a marker of exosomes secreted into urine and amniotic fluid.
Authors "Keller S, Rupp C, Stoeck A, Runz S, Fogel M, Lugert S, Hager HD, Abdel-Bakky MS, Gutwein P, Altevogt P"
Journal name KI
Publication year 2007
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.05-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
FACS
 Protein-protein interactions for CD24
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FGR 2268
Affinity Capture-Western Homo sapiens
2 LYN 4067
Affinity Capture-Western Homo sapiens
3 SELP 6403
Reconstituted Complex Homo sapiens
4 NPM1 4869
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which CD24 is involved
PathwayEvidenceSource
Axon guidance IEA Reactome
Developmental Biology IEA Reactome
L1CAM interactions IEA Reactome
Nervous system development IEA Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here