Gene description for TNFAIP6
Gene name tumor necrosis factor, alpha-induced protein 6
Gene symbol TNFAIP6
Other names/aliases TSG-6
TSG6
Species Homo sapiens
 Database cross references - TNFAIP6
ExoCarta ExoCarta_7130
Vesiclepedia VP_7130
Entrez Gene 7130
HGNC 11898
MIM 600410
UniProt P98066  
 TNFAIP6 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
 Gene ontology annotations for TNFAIP6
Molecular Function
    fibronectin binding GO:0001968 IDA
    calcium ion binding GO:0005509 IDA
    protein binding GO:0005515 IPI
    hyaluronic acid binding GO:0005540 IDA
    carboxylesterase activity GO:0106435 IDA
Biological Process
    ovarian cumulus expansion GO:0001550 ISS
    inflammatory response GO:0006954 TAS
    cell adhesion GO:0007155 IEA
    signal transduction GO:0007165 TAS
    cell-cell signaling GO:0007267 TAS
    hyaluronan metabolic process GO:0030212 IDA
    positive regulation of cell migration GO:0030335 IEA
    negative regulation of BMP signaling pathway GO:0030514 IDA
    negative regulation of osteoblast differentiation GO:0045668 IDA
    negative regulation of osteoclast differentiation GO:0045671 IDA
    negative regulation of inflammatory response GO:0050728 IBA
    negative regulation of inflammatory response GO:0050728 IDA
    negative regulation of neutrophil chemotaxis GO:0090024 IDA
    positive regulation of receptor clustering GO:1903911 IDA
    fibronectin fibril organization GO:1905590 IDA
Subcellular Localization
    extracellular region GO:0005576 IDA
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 IBA
    tertiary granule lumen GO:1904724 TAS
    ficolin-1-rich granule lumen GO:1904813 TAS
 Experiment description of studies that identified TNFAIP6 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for TNFAIP6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 THOC7 80145
Affinity Capture-MS Homo sapiens
2 VCAN 1462
Affinity Capture-MS Homo sapiens
3 WDR11 55717
Affinity Capture-MS Homo sapiens
4 TXNDC16 57544
Affinity Capture-MS Homo sapiens
5 THOC1 9984
Affinity Capture-MS Homo sapiens
6 RNF166  
Affinity Capture-MS Homo sapiens
7 FOXRED2  
Affinity Capture-MS Homo sapiens
8 CTPS2 56474
Affinity Capture-MS Homo sapiens
9 RPAP2  
Affinity Capture-MS Homo sapiens
10 UBC 7316
Reconstituted Complex Homo sapiens
11 ITIH2 3698
Affinity Capture-MS Homo sapiens
12 C17orf75 64149
Affinity Capture-MS Homo sapiens
13 THOC5 8563
Affinity Capture-MS Homo sapiens
14 KIF7 374654
Affinity Capture-MS Homo sapiens
15 ACAN 176
Reconstituted Complex Homo sapiens
16 ETNPPL  
Affinity Capture-MS Homo sapiens
17 FAM120B 84498
Affinity Capture-MS Homo sapiens
18 ZMYM6  
Affinity Capture-MS Homo sapiens
19 ACADL 33
Affinity Capture-MS Homo sapiens
20 ITIH3 3699
Affinity Capture-MS Homo sapiens
21 LRP2 4036
Affinity Capture-MS Homo sapiens
22 SUPV3L1 6832
Affinity Capture-MS Homo sapiens
23 TBC1D23 55773
Affinity Capture-MS Homo sapiens
24 KCTD21 283219
Affinity Capture-MS Homo sapiens
25 FAM91A1 157769
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which TNFAIP6 is involved
PathwayEvidenceSource
Immune System TAS Reactome
Innate Immune System TAS Reactome
Neutrophil degranulation TAS Reactome





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