Gene description for LGALS7
Gene name lectin, galactoside-binding, soluble, 7
Gene symbol LGALS7
Other names/aliases GAL7
LGALS7A
Species Homo sapiens
 Database cross references - LGALS7
ExoCarta ExoCarta_3963
Vesiclepedia VP_3963
Entrez Gene 3963
HGNC 6568
MIM 600615
UniProt P47929  
 LGALS7 identified in exosomes derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Hepatocellular carcinoma cells 26054723    
Keratinocytes 19530224    
Keratinocytes 19530224    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Saliva 19199708    
Thymus 23844026    
 Gene ontology annotations for LGALS7
 Experiment description of studies that identified LGALS7 in exosomes
1
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
9
Experiment ID 189
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19530224    
Organism Homo sapiens
Experiment description Profile of exosomes related proteins released by differentiated and undifferentiated human keratinocytes.
Authors "Chavez-Muñoz C, Kilani RT, Ghahary A."
Journal name J Cell Physiol
Publication year 2009
Sample Keratinocytes
Sample name Keratinocytes - Differentiated
Isolation/purification methods Differential centrifugation
Filtration
Sucrose cushion
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 190
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19530224    
Organism Homo sapiens
Experiment description Profile of exosomes related proteins released by differentiated and undifferentiated human keratinocytes.
Authors "Chavez-Muñoz C, Kilani RT, Ghahary A."
Journal name J Cell Physiol
Publication year 2009
Sample Keratinocytes
Sample name Keratinocytes - Undifferentiated
Isolation/purification methods Differential centrifugation
Filtration
Sucrose cushion
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
13
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
14
Experiment ID 66
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|Alix|CD63|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR"
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
15
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for LGALS7
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SERPINB4 6318
Affinity Capture-MS Homo sapiens
2 HSPA6 3310
Two-hybrid Homo sapiens
3 MTPAP 55149
Affinity Capture-MS Homo sapiens
4 TUBB8 347688
Affinity Capture-MS Homo sapiens
5 LNPEP 4012
Affinity Capture-MS Homo sapiens
6 CTSD 1509
Affinity Capture-MS Homo sapiens
7 RHEB 6009
Affinity Capture-MS Homo sapiens
8 SCARA3  
Affinity Capture-MS Homo sapiens
9 APAF1 317
Affinity Capture-MS Homo sapiens
10 DPYSL2 1808
Affinity Capture-MS Homo sapiens
11 MCM2 4171
Affinity Capture-MS Homo sapiens
12 PTPRJ 5795
Affinity Capture-MS Homo sapiens
13 COPS5 10987
Affinity Capture-MS Homo sapiens
14 FBXW7  
Affinity Capture-MS Homo sapiens
15 IVL 3713
Affinity Capture-MS Homo sapiens
16 KRI1  
Affinity Capture-MS Homo sapiens
17 PIK3C3 5289
Affinity Capture-MS Homo sapiens
18 PRDM4  
Affinity Capture-MS Homo sapiens
19 TUBA3C 7278
Affinity Capture-MS Homo sapiens
20 NID2 22795
Affinity Capture-MS Homo sapiens
21 SFN 2810
Affinity Capture-MS Homo sapiens
22 PKN2 5586
Affinity Capture-MS Homo sapiens
23 SLC12A7 10723
Affinity Capture-MS Homo sapiens
24 ADSS 159
Affinity Capture-MS Homo sapiens
25 LSM2 57819
Two-hybrid Homo sapiens
26 INSIG2  
Affinity Capture-MS Homo sapiens
27 PALD1 27143
Affinity Capture-MS Homo sapiens
28 DDX19B 11269
Affinity Capture-MS Homo sapiens
29 CHMP4C 92421
Affinity Capture-MS Homo sapiens
30 ACTA2 59
Affinity Capture-MS Homo sapiens
31 SDK2 54549
Affinity Capture-MS Homo sapiens
32 POF1B 79983
Affinity Capture-MS Homo sapiens
33 DPYSL4 10570
Affinity Capture-MS Homo sapiens
34 APLP2 334
Affinity Capture-MS Homo sapiens
35 POLA2  
Affinity Capture-MS Homo sapiens
36 SDK1 221935
Affinity Capture-MS Homo sapiens
37 CSPG4 1464
Affinity Capture-MS Homo sapiens
38 RCCD1  
Affinity Capture-MS Homo sapiens
39 TRIM21 6737
Affinity Capture-MS Homo sapiens
40 SLX4  
Affinity Capture-MS Homo sapiens
41 S100A8 6279
Affinity Capture-MS Homo sapiens
42 WIPI1 55062
Affinity Capture-MS Homo sapiens
43 TGM5  
Two-hybrid Homo sapiens
44 DDX39B 7919
Affinity Capture-MS Homo sapiens
45 CYLD  
Affinity Capture-MS Homo sapiens
46 VCAN 1462
Affinity Capture-MS Homo sapiens
47 CALML3 810
Affinity Capture-MS Homo sapiens
48 SERPINB3 6317
Affinity Capture-MS Homo sapiens
49 USP4 7375
Affinity Capture-MS Homo sapiens
50 FANCD2  
Affinity Capture-MS Homo sapiens
51 POLE2  
Affinity Capture-MS Homo sapiens
52 DOCK8 81704
Affinity Capture-MS Homo sapiens
53 XPO1 7514
Affinity Capture-MS Homo sapiens
54 USP15 9958
Affinity Capture-MS Homo sapiens
55 DOCK7 85440
Affinity Capture-MS Homo sapiens
56 JADE1  
Affinity Capture-MS Homo sapiens
57 USP38 84640
Affinity Capture-MS Homo sapiens
58 LAMA1 284217
Affinity Capture-MS Homo sapiens
59 HNRNPA1 3178
Affinity Capture-MS Homo sapiens
60 PKD1 5310
Affinity Capture-MS Homo sapiens
61 SUZ12  
Affinity Capture-MS Homo sapiens
62 FN1 2335
Affinity Capture-MS Homo sapiens
63 GAB1  
Affinity Capture-MS Homo sapiens
64 TUBA4A 7277
Affinity Capture-MS Homo sapiens
65 CDK2 1017
Affinity Capture-MS Homo sapiens
66 FABP5 2171
Affinity Capture-MS Homo sapiens
67 HECW2  
Affinity Capture-MS Homo sapiens
68 IGSF3 3321
Affinity Capture-MS Homo sapiens
69 LGALS7B 653499
Affinity Capture-MS Homo sapiens
70 LAMA4 3910
Affinity Capture-MS Homo sapiens
71 PODXL 5420
Affinity Capture-MS Homo sapiens
72 SFMBT1 51460
Affinity Capture-MS Homo sapiens
73 SERPINB5 5268
Affinity Capture-MS Homo sapiens
74 DSP 1832
Affinity Capture-MS Homo sapiens
75 LAMB1 3912
Affinity Capture-MS Homo sapiens
76 SETD4 54093
Affinity Capture-MS Homo sapiens
77 KIAA1549  
Affinity Capture-MS Homo sapiens
78 COLEC12 81035
Affinity Capture-MS Homo sapiens
79 JUP 3728
Affinity Capture-MS Homo sapiens
80 MKS1 54903
Affinity Capture-MS Homo sapiens
81 VPS13B  
Affinity Capture-MS Homo sapiens
82 TUBG1 7283
Affinity Capture-MS Homo sapiens
83 DPYSL5  
Affinity Capture-MS Homo sapiens
84 KLHL8  
Affinity Capture-MS Homo sapiens
85 USP1 7398
Affinity Capture-MS Homo sapiens
86 KAT7  
Affinity Capture-MS Homo sapiens
87 VWA9  
Affinity Capture-MS Homo sapiens
88 TUBB3 10381
Affinity Capture-MS Homo sapiens
89 SQSTM1 8878
Proximity Label-MS Homo sapiens
90 G3BP1 10146
Affinity Capture-MS Homo sapiens
91 HSPB1 3315
Affinity Capture-MS Homo sapiens
92 S100A9 6280
Affinity Capture-MS Homo sapiens
93 SSBP1 6742
Two-hybrid Homo sapiens
94 ESR1  
Affinity Capture-MS Homo sapiens
95 KRR1 11103
Affinity Capture-MS Homo sapiens
96 NAMPT 10135
Affinity Capture-MS Homo sapiens
97 EZH2  
Affinity Capture-MS Homo sapiens
98 CALML5 51806
Affinity Capture-MS Homo sapiens
99 LUCAT1  
Affinity Capture-RNA Homo sapiens
100 PINK1  
Affinity Capture-MS Homo sapiens
101 TCF3  
Affinity Capture-MS Homo sapiens
102 METTL3  
Affinity Capture-MS Homo sapiens
103 CRYM 1428
Affinity Capture-MS Homo sapiens
104 PTPRK 5796
Affinity Capture-MS Homo sapiens
105 ANXA2 302
Affinity Capture-MS Homo sapiens
106 HEG1 57493
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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