Gene description for CRYM
Gene name crystallin, mu
Gene symbol CRYM
Other names/aliases DFNA40
THBP
Species Homo sapiens
 Database cross references - CRYM
ExoCarta ExoCarta_1428
Vesiclepedia VP_1428
Entrez Gene 1428
HGNC 2418
MIM 123740
UniProt Q14894  
 CRYM identified in sEVs derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Urine 19056867    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for CRYM
Molecular Function
    transcription corepressor activity GO:0003714 IMP
    protein binding GO:0005515 IPI
    protein homodimerization activity GO:0042803 IDA
    thiomorpholine-carboxylate dehydrogenase activity GO:0047127 IDA
    thiomorpholine-carboxylate dehydrogenase activity GO:0047127 TAS
    NADP binding GO:0050661 IDA
    thyroid hormone binding GO:0070324 IBA
    thyroid hormone binding GO:0070324 IDA
    thyroid hormone binding GO:0070324 IMP
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IMP
    lysine catabolic process GO:0006554 TAS
    sensory perception of sound GO:0007605 IMP
    thyroid hormone metabolic process GO:0042403 IBA
    thyroid hormone transport GO:0070327 IMP
Subcellular Localization
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    mitochondrion GO:0005739 IEA
    peroxisomal matrix GO:0005782 TAS
    cytosol GO:0005829 IDA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified CRYM in sEVs
1
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
4
Experiment ID 196
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 197
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CRYM
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FCGR2B  
Affinity Capture-MS Homo sapiens
2 ADRB2  
Two-hybrid Homo sapiens
3 C7orf25  
Two-hybrid Homo sapiens
4 CDC37 11140
Two-hybrid Homo sapiens
5 RNF126  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 TERF1 7013
Two-hybrid Homo sapiens
7 NSF 4905
Affinity Capture-MS Homo sapiens
8 LGALS7 3963
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which CRYM is involved
PathwayEvidenceSource
Lysine catabolism TAS Reactome
Metabolism TAS Reactome
Metabolism of amino acids and derivatives TAS Reactome





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