Experiment description of studies that identified IGSF3 in exosomes

1 Experiment ID 21 ISEV standards ✔ EM|IEM EV Biophysical techniques ✔ Alix|TSG101|HSP70 EV Cytosolic markers ✔ CD63 EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry    PubMed ID 19837982     Organism Homo sapiens Experiment description Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215. Authors Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson Journal name MCP Publication year 2009 Sample Colorectal cancer cells Sample name LIM1215 Isolation/purification methods Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.10-1.12 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry [Orbitrap] Western blotting

2 Experiment ID 201 ISEV standards ✔ EM EV Biophysical techniques ✔ Alix|TSG101|HSP70 EV Cytosolic markers ✔ CD9 EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 22740476     Organism Homo sapiens Experiment description Restoration of full-length APC protein in SW480 colon cancer cells induces exosome-mediated secretion of DKK-4. Authors Lim JW, Mathias RA, Kapp EA, Layton MJ, Faux MC, Burgess AW, Ji H, Simpson RJ. Journal name Electrophoresis Publication year 2012 Sample Colorectal cancer cells Sample name SW480 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

3 Experiment ID 254 ISEV standards ✔ EM EV Biophysical techniques ✔ TSG101 EV Cytosolic markers ✔ FLOT1|CD81 EV Membrane markers ✘ EV Negative markers ✔ NTA EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25950383     Organism Homo sapiens Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L Journal name Pigment Cell Melanoma Res Publication year 2015 Sample Melanoma cells Sample name MNT-1 Isolation/purification methods Differential centrifugation Unltracentrifugation Sucrose density gradient Flotation density 1.13 - 1.19 g/mL Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

4 Experiment ID 255 ISEV standards ✔ EM EV Biophysical techniques ✔ TSG101 EV Cytosolic markers ✔ FLOT1 EV Membrane markers ✘ EV Negative markers ✔ NTA EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25950383     Organism Homo sapiens Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L Journal name Pigment Cell Melanoma Res Publication year 2015 Sample Melanoma cells Sample name G1 Isolation/purification methods Differential centrifugation Unltracentrifugation Sucrose density gradient Flotation density 1.13 - 1.19 g/mL Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

5 Experiment ID 256 ISEV standards ✔ EM EV Biophysical techniques ✔ TSG101 EV Cytosolic markers ✔ FLOT1 EV Membrane markers ✘ EV Negative markers ✔ NTA EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25950383     Organism Homo sapiens Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L Journal name Pigment Cell Melanoma Res Publication year 2015 Sample Melanoma cells Sample name 501mel Isolation/purification methods Differential centrifugation Unltracentrifugation Sucrose density gradient Flotation density 1.13 - 1.19 g/mL Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

6 Experiment ID 257 ISEV standards ✔ EM EV Biophysical techniques ✔ TSG101 EV Cytosolic markers ✔ FLOT1 EV Membrane markers ✘ EV Negative markers ✔ NTA EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25950383     Organism Homo sapiens Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L Journal name Pigment Cell Melanoma Res Publication year 2015 Sample Melanoma cells Sample name Daju Isolation/purification methods Differential centrifugation Unltracentrifugation Sucrose density gradient Flotation density 1.13 - 1.19 g/mL Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

7 Experiment ID 258 ISEV standards ✔ EM EV Biophysical techniques ✔ TSG101 EV Cytosolic markers ✔ FLOT1|CD81 EV Membrane markers ✘ EV Negative markers ✔ NTA EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25950383     Organism Homo sapiens Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L Journal name Pigment Cell Melanoma Res Publication year 2015 Sample Melanoma cells Sample name SKMEL28 Isolation/purification methods Differential centrifugation Unltracentrifugation Sucrose density gradient Flotation density 1.13 - 1.19 g/mL Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

8 Experiment ID 259 ISEV standards ✔ EM EV Biophysical techniques ✔ TSG101 EV Cytosolic markers ✔ FLOT1 EV Membrane markers ✘ EV Negative markers ✔ NTA EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25950383     Organism Homo sapiens Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L Journal name Pigment Cell Melanoma Res Publication year 2015 Sample Melanoma cells Sample name A375M Isolation/purification methods Differential centrifugation Unltracentrifugation Sucrose density gradient Flotation density 1.13 - 1.19 g/mL Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

9 Experiment ID 260 ISEV standards ✔ EM EV Biophysical techniques ✔ TSG101 EV Cytosolic markers ✔ FLOT1|CD81 EV Membrane markers ✘ EV Negative markers ✔ NTA EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25950383     Organism Homo sapiens Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L Journal name Pigment Cell Melanoma Res Publication year 2015 Sample Melanoma cells Sample name 1205Lu Isolation/purification methods Differential centrifugation Unltracentrifugation Sucrose density gradient Flotation density 1.13 - 1.19 g/mL Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

10 Experiment ID 224 ISEV standards ✔ EM|AFM EV Biophysical techniques ✔ Alix|TSG101 EV Cytosolic markers ✔ CD63|CD81 EV Membrane markers ✔ GOLGA2 EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25944692     Organism Homo sapiens Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S Journal name Oncotarget Publication year 2015 Sample Neuroblastoma cells Sample name SH-SY5Y Isolation/purification methods Differential centrifugation Ultracentrifugation OptiPrep density gradient Flotation density 1.10 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting

11 Experiment ID 211 ISEV standards ✔ EM EV Biophysical techniques ✔ TSG101|Alix EV Cytosolic markers ✔ EpCAM|TFRC EV Membrane markers ✔ cytochrome c|GOLGA2 EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23333927     Organism Homo sapiens Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes. Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K. Journal name J Proteomics Publication year 2013 Sample Ovarian cancer cells Sample name IGROV1 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.09-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

12 Experiment ID 212 ISEV standards ✔ CEM EV Biophysical techniques ✔ TSG101|Alix EV Cytosolic markers ✔ EpCAM|TFRC EV Membrane markers ✔ Cytochrome C|GOLGA2 EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23333927     Organism Homo sapiens Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes. Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K. Journal name J Proteomics Publication year 2013 Sample Ovarian cancer cells Sample name OVCAR-3 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.09-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

13 Experiment ID 275 ISEV standards ✔ EM EV Biophysical techniques ✔ TSG101|Alix|RAB5A EV Cytosolic markers ✔ CD9|CD82|CD63|CD81 EV Membrane markers ✔ AIF EV Negative markers ✔ NTA EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25844599     Organism Homo sapiens Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel. Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T. Journal name Oncotarget Publication year 2015 Sample Prostate cancer cells Sample name DU145 - Docetaxel sensitive Isolation/purification methods Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.12-1.19 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry/Flow cytometry/Western blotting

14 Experiment ID 274 ISEV standards ✔ EM EV Biophysical techniques ✔ TSG101|Alix|RAB5A EV Cytosolic markers ✔ CD9|CD82|CD63|CD81 EV Membrane markers ✔ AIF EV Negative markers ✔ NTA EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25844599     Organism Homo sapiens Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel. Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T. Journal name Oncotarget Publication year 2015 Sample Prostate cancer cells Sample name DU145 - Docetaxel resistant Isolation/purification methods Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.18 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry Flow cytometry Western blotting

Protein-protein interactions for IGSF3

Protein Interactor ExoCarta ID Identification method PubMed Species No interactions are found.

Pathways in which IGSF3 is involved

No pathways found