Gene description for TUBA4A
Gene name tubulin, alpha 4a
Gene symbol TUBA4A
Other names/aliases ALS22
H2-ALPHA
TUBA1
Species Homo sapiens
 Database cross references - TUBA4A
ExoCarta ExoCarta_7277
Vesiclepedia VP_7277
Entrez Gene 7277
HGNC 12407
MIM 191110
UniProt P68366  
 TUBA4A identified in sEVs derived from the following tissue/cell type
B cells 20458337    
B cells 20458337    
B cells 20458337    
Bladder cancer cells 20224111    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 17956143    
Colorectal cancer cells 19837982    
Colorectal cancer cells 22740476    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 25890246    
Colorectal cancer cells 34887515    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Foreskin fibroblasts 34108659    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Keratinocytes 19530224    
Keratinocytes 19530224    
Lymphoma cells 34108659    
Mammary cancer-associated fibroblasts 34108659    
Monocytic leukemia cells 34108659    
Neuroblastoma cells 25944692    
Normal mammary epithelial cells 34108659    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic duct epithalial cells 34108659    
Pancreatic duct epithalial cells 34108659    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Pluripotent stem cells 34108659    
Prostate cancer cells 25844599    
Prostate cancer cells 25844599    
Saliva 19199708    
Squamous carcinoma cells 20124223    
T lymphocytes 34108659    
Thymus 23844026    
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for TUBA4A
Molecular Function
    structural constituent of cytoskeleton GO:0005200 IBA
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IBA
    hydrolase activity GO:0016787 IEA
    protein kinase binding GO:0019901 IPI
    metal ion binding GO:0046872 IEA
Biological Process
    microtubule cytoskeleton organization GO:0000226 IBA
    mitotic cell cycle GO:0000278 IBA
Subcellular Localization
    extracellular region GO:0005576 TAS
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 TAS
    cytoskeleton GO:0005856 IDA
    microtubule GO:0005874 IBA
    microtubule GO:0005874 IDA
    microtubule cytoskeleton GO:0015630 IDA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified TUBA4A in sEVs
1
Experiment ID 79
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
2
Experiment ID 80
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 81
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
4
Experiment ID 76
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|GAPDH|HSP90|CD81|CD9|CD63|LAMP1|MHCI
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20224111    
Organism Homo sapiens
Experiment description Proteomics analysis of bladder cancer exosomes.
Authors "Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A."
Journal name MCP
Publication year 2010
Sample Bladder cancer cells
Sample name HT1376
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
FACS
5
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 412
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MCF7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
10
Experiment ID 414
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
11
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
13
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
14
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
15
Experiment ID 20
MISEV standards
✔
EM
Biophysical techniques
✔
HSP90|CD63|CD81|LAMP1
Enriched markers
✔
GOLGA2|cytochrome c
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17956143    
Organism Homo sapiens
Experiment description Proteomic analysis of microvesicles derived from human colorectal cancer cells.
Authors "Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS"
Journal name JPR
Publication year 2007
Sample Colorectal cancer cells
Sample name HT29
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Diafiltration
Flotation density 1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
16
Experiment ID 21
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry   
PubMed ID 19837982    
Organism Homo sapiens
Experiment description Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215.
Authors "Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson"
Journal name MCP
Publication year 2009
Sample Colorectal cancer cells
Sample name LIM1215
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.10-1.12 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [Orbitrap]
Western blotting
17
Experiment ID 201
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22740476    
Organism Homo sapiens
Experiment description Restoration of full-length APC protein in SW480 colon cancer cells induces exosome-mediated secretion of DKK-4.
Authors "Lim JW, Mathias RA, Kapp EA, Layton MJ, Faux MC, Burgess AW, Ji H, Simpson RJ."
Journal name Electrophoresis
Publication year 2012
Sample Colorectal cancer cells
Sample name SW480
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
18
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
19
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
20
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
21
Experiment ID 286
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Sequential centrifugal ultrafiltration - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Sequential centrifugal ultrafiltration
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
22
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
23
Experiment ID 407
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|TSG101|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
24
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
25
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
26
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
27
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
28
Experiment ID 405
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Foreskin fibroblasts
Sample name BJ
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
29
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
30
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
31
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
32
Experiment ID 189
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19530224    
Organism Homo sapiens
Experiment description Profile of exosomes related proteins released by differentiated and undifferentiated human keratinocytes.
Authors "Chavez-Muñoz C, Kilani RT, Ghahary A."
Journal name J Cell Physiol
Publication year 2009
Sample Keratinocytes
Sample name Keratinocytes - Differentiated
Isolation/purification methods Differential centrifugation
Filtration
Sucrose cushion
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
33
Experiment ID 190
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19530224    
Organism Homo sapiens
Experiment description Profile of exosomes related proteins released by differentiated and undifferentiated human keratinocytes.
Authors "Chavez-Muñoz C, Kilani RT, Ghahary A."
Journal name J Cell Physiol
Publication year 2009
Sample Keratinocytes
Sample name Keratinocytes - Undifferentiated
Isolation/purification methods Differential centrifugation
Filtration
Sucrose cushion
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
34
Experiment ID 417
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Lymphoma cells
Sample name Raji
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
35
Experiment ID 411
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Mammary cancer-associated fibroblasts
Sample name mCAF
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
36
Experiment ID 418
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Monocytic leukemia cells
Sample name THP-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
37
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
38
Experiment ID 413
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Normal mammary epithelial cells
Sample name MCF10A
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
39
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
40
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
41
Experiment ID 406
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35|CD81
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name BxPC3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
42
Experiment ID 415
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
43
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
44
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
45
Experiment ID 408
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPDE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
46
Experiment ID 409
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPNE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
47
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
48
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
49
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
50
Experiment ID 416
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pluripotent stem cells
Sample name PSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
51
Experiment ID 275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
52
Experiment ID 274
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
53
Experiment ID 66
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|Alix|CD63|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR"
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
54
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
55
Experiment ID 410
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample T lymphocytes
Sample name Jurkat
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
56
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
57
Experiment ID 193
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD9
Enriched markers
✔
PHB
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
58
Experiment ID 194
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
59
Experiment ID 195
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for TUBA4A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HDAC4  
Affinity Capture-MS Homo sapiens
2 NLRP1  
Affinity Capture-MS Homo sapiens
3 PKM 5315
Co-fractionation Homo sapiens
4 SKI 6497
Affinity Capture-MS Homo sapiens
5 CSNK1A1 1452
Affinity Capture-MS Homo sapiens
6 VHL  
Affinity Capture-MS Homo sapiens
7 LOXL4 84171
Affinity Capture-MS Homo sapiens
8 SOX2  
Affinity Capture-MS Homo sapiens
9 POLH  
Affinity Capture-MS Homo sapiens
10 KIF23 9493
Affinity Capture-Western Homo sapiens
11 UBE2O 63893
Affinity Capture-MS Homo sapiens
12 FCN3 8547
Affinity Capture-MS Homo sapiens
13 NIT1 4817
Affinity Capture-MS Homo sapiens
14 GLMP 112770
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 ST14 6768
Affinity Capture-MS Homo sapiens
16 KLHL33  
Affinity Capture-MS Homo sapiens
17 MAGED2 10916
Affinity Capture-MS Homo sapiens
18 ILK 3611
Affinity Capture-MS Homo sapiens
19 UCHL5 51377
Reconstituted Complex Homo sapiens
20 IL17RA 23765
Affinity Capture-MS Homo sapiens
21 PRTN3 5657
Affinity Capture-MS Homo sapiens
22 TSHB  
Affinity Capture-MS Homo sapiens
23 EEF1A1 1915
Co-fractionation Homo sapiens
24 DHDH  
Affinity Capture-MS Homo sapiens
25 CHMP4C 92421
Affinity Capture-MS Homo sapiens
26 DNAL4  
Affinity Capture-MS Homo sapiens
27 RIN3  
Affinity Capture-MS Homo sapiens
28 YAP1 10413
Affinity Capture-MS Homo sapiens
29 MYH9 4627
Co-fractionation Homo sapiens
30 TYK2 7297
Affinity Capture-MS Homo sapiens
31 OGT 8473
Reconstituted Complex Homo sapiens
32 PEX14 5195
Co-purification Homo sapiens
33 CUL2 8453
Affinity Capture-MS Homo sapiens
34 PHLPP1  
Proximity Label-MS Homo sapiens
35 HSPA5 3309
Co-fractionation Homo sapiens
36 ADAMTS4  
Affinity Capture-MS Homo sapiens
37 CD79B 974
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 LRRC25  
Affinity Capture-MS Homo sapiens
39 IQCB1  
Affinity Capture-MS Homo sapiens
40 TUBAL3 79861
Affinity Capture-MS Homo sapiens
41 PRKCZ 5590
Affinity Capture-MS Homo sapiens
42 MADD 8567
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 OPRL1 4987
Affinity Capture-MS Homo sapiens
44 BPNT1 10380
Affinity Capture-MS Homo sapiens
45 ALDH3B1 221
Affinity Capture-MS Homo sapiens
46 TXNDC9 10190
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 RPA3 6119
Affinity Capture-MS Homo sapiens
48 TUBGCP2 10844
Affinity Capture-MS Homo sapiens
49 LYPD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 CDC20 991
Affinity Capture-MS Homo sapiens
51 OBSCN 84033
Affinity Capture-MS Homo sapiens
52 HSPA1A 3303
Co-fractionation Homo sapiens
53 TCP11L2  
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
54 TUBB7P 56604
Co-fractionation Homo sapiens
55 MAGEA8  
Affinity Capture-MS Homo sapiens
56 YBX1 4904
Co-fractionation Homo sapiens
57 RPS27A 6233
Co-fractionation Homo sapiens
58 RHBDF2 79651
Affinity Capture-MS Homo sapiens
59 NAAA  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 SLC18A1  
Affinity Capture-MS Homo sapiens
61 RUVBL2 10856
Co-fractionation Homo sapiens
62 ZEB1  
Affinity Capture-MS Homo sapiens
63 CD1A 909
Affinity Capture-MS Homo sapiens
64 CHRM4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 PDDC1 347862
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 TKT 7086
Affinity Capture-MS Homo sapiens
67 EFNB3 1949
Affinity Capture-MS Homo sapiens
68 CAPS 828
Affinity Capture-MS Homo sapiens
69 ARSG 22901
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 CST11  
Affinity Capture-MS Homo sapiens
71 SNW1 22938
Affinity Capture-MS Homo sapiens
72 PPP2R2A 5520
Affinity Capture-MS Homo sapiens
73 UBE3D  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
74 UGGT2 55757
Affinity Capture-MS Homo sapiens
75 C5AR2  
Affinity Capture-MS Homo sapiens
76 SPRTN  
Affinity Capture-MS Homo sapiens
77 CEACAM8 1088
Affinity Capture-MS Homo sapiens
78 CNTNAP3  
Affinity Capture-MS Homo sapiens
79 CD48 962
Affinity Capture-MS Homo sapiens
80 KLK5 25818
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
81 RPA2 6118
Affinity Capture-MS Homo sapiens
82 CRYL1 51084
Affinity Capture-MS Homo sapiens
83 APC  
Co-localization Homo sapiens
Reconstituted Complex Homo sapiens
84 CCT6B 10693
Affinity Capture-MS Homo sapiens
85 CAND1 55832
Affinity Capture-MS Homo sapiens
86 NDUFA4 4697
Affinity Capture-MS Homo sapiens
87 MFAP5  
Affinity Capture-MS Homo sapiens
88 SPG7  
Affinity Capture-MS Homo sapiens
89 MUC20  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
90 LRP1 4035
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
91 MBIP  
Affinity Capture-MS Homo sapiens
92 PGK1 5230
Co-fractionation Homo sapiens
93 B3GNT2 10678
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
94 COPS6 10980
Affinity Capture-MS Homo sapiens
95 C9orf3 84909
Affinity Capture-MS Homo sapiens
96 SRRM2 23524
Affinity Capture-MS Homo sapiens
97 MLST8 64223
Affinity Capture-MS Homo sapiens
98 TULP2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
99 OBSL1 23363
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
100 C2CD2 25966
Affinity Capture-MS Homo sapiens
101 VCAM1 7412
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
102 FAM196A  
Affinity Capture-MS Homo sapiens
103 FOXD4  
Affinity Capture-MS Homo sapiens
104 KLK6 5653
Affinity Capture-MS Homo sapiens
105 CUL1 8454
Affinity Capture-MS Homo sapiens
106 FGFR1 2260
Affinity Capture-MS Homo sapiens
107 TUBB6 84617
Co-fractionation Homo sapiens
108 DLGAP5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
109 PRNP 5621
Affinity Capture-MS Homo sapiens
110 ITLN1 55600
Affinity Capture-MS Homo sapiens
111 CCT2 10576
Affinity Capture-MS Homo sapiens
112 VSIG1  
Affinity Capture-MS Homo sapiens
113 BSG 682
Affinity Capture-MS Homo sapiens
114 PRPF8 10594
Co-fractionation Homo sapiens
115 IGH 3492
Affinity Capture-MS Homo sapiens
116 BAG6 7917
Affinity Capture-Western Homo sapiens
117 PLD2 5338
Affinity Capture-MS Homo sapiens
118 TUBB2B 347733
Co-fractionation Homo sapiens
119 TUBA1C 84790
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
120 CRISP2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
121 THBS3 7059
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
122 GML  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
123 PRKAB1 5564
Affinity Capture-MS Homo sapiens
124 ICAM5 7087
Affinity Capture-MS Homo sapiens
125 CYLD  
Affinity Capture-MS Homo sapiens
126 BRINP3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
127 NYX  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
128 FBXL4 26235
Affinity Capture-MS Homo sapiens
129 SLC15A1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
130 TUBB 203068
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
131 AIFM1 9131
Affinity Capture-MS Homo sapiens
132 ICAM1 3383
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
133 XPO1 7514
Affinity Capture-MS Homo sapiens
134 RC3H2  
Affinity Capture-MS Homo sapiens
135 KLK11 11012
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
136 PRG2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
137 TUBB4B 10383
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
138 EGFR 1956
Affinity Capture-MS Homo sapiens
139 AQP3  
Affinity Capture-MS Homo sapiens
140 LGALS7 3963
Affinity Capture-MS Homo sapiens
141 INSL5  
Affinity Capture-MS Homo sapiens
142 RPS27 6232
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
143 MYC  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
144 BAG1 573
Affinity Capture-MS Homo sapiens
145 IL12RB1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
146 DAAM1 23002
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
147 CDK20  
Affinity Capture-MS Homo sapiens
148 TRAC  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
149 NXPH3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
150 CST9L  
Affinity Capture-MS Homo sapiens
151 MIER2  
Affinity Capture-MS Homo sapiens
152 SERPINB11  
Affinity Capture-MS Homo sapiens
153 LYZL2  
Affinity Capture-MS Homo sapiens
154 EIF3F 8665
Affinity Capture-MS Homo sapiens
155 IRAK2  
Affinity Capture-MS Homo sapiens
156 PRKCSH 5589
Affinity Capture-MS Homo sapiens
157 PRKY  
Affinity Capture-MS Homo sapiens
158 PSG1  
Affinity Capture-MS Homo sapiens
159 ILF3 3609
Co-fractionation Homo sapiens
160 TTC5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
161 TP53 7157
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
162 FOLR1 2348
Affinity Capture-MS Homo sapiens
163 PSMC4 5704
Co-fractionation Homo sapiens
164 MFI2 4241
Affinity Capture-MS Homo sapiens
165 SLAMF1 6504
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
166 HNRNPK 3190
Co-fractionation Homo sapiens
167 CHRM3 1131
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
168 JAK3 3718
Affinity Capture-MS Homo sapiens
169 RBM23  
Two-hybrid Homo sapiens
170 TMEM25  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
171 BCL2L1 598
Affinity Capture-MS Homo sapiens
172 NUP210P1  
Affinity Capture-MS Homo sapiens
173 PSMD1 5707
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
174 VASN 114990
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
175 ATOH1  
Affinity Capture-MS Homo sapiens
176 CUL4A 8451
Affinity Capture-MS Homo sapiens
177 TCP1 6950
Affinity Capture-MS Homo sapiens
178 BRINP2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
179 SENP3 26168
Affinity Capture-MS Homo sapiens
180 C2CD4A  
Affinity Capture-MS Homo sapiens
181 GDF9 2661
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
182 IGHM 3507
Affinity Capture-MS Homo sapiens
183 SLURP1  
Affinity Capture-MS Homo sapiens
184 CEP97 79598
Affinity Capture-MS Homo sapiens
185 CUL4B 8450
Affinity Capture-MS Homo sapiens
186 CELA3A  
Affinity Capture-MS Homo sapiens
187 CUL7 9820
Affinity Capture-MS Homo sapiens
188 HYAL1 3373
Affinity Capture-MS Homo sapiens
189 RUVBL1 8607
Co-fractionation Homo sapiens
190 CRYZ 1429
Co-fractionation Homo sapiens
191 MCM2 4171
Affinity Capture-MS Homo sapiens
192 HLA-B 3106
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
193 BAGE2  
Affinity Capture-MS Homo sapiens
194 HCFC2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
195 SF3B3 23450
Affinity Capture-MS Homo sapiens
196 ST8SIA4 7903
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
197 ATG16L1 55054
Affinity Capture-MS Homo sapiens
198 TCP11L1 55346
Affinity Capture-MS Homo sapiens
199 TFCP2 7024
Affinity Capture-MS Homo sapiens
200 INSIG2  
Affinity Capture-MS Homo sapiens
201 TSHR 7253
Affinity Capture-MS Homo sapiens
202 NSUN2 54888
Affinity Capture-MS Homo sapiens
203 PLAUR 5329
Affinity Capture-MS Homo sapiens
204 CHIA  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
205 HCST  
Affinity Capture-MS Homo sapiens
206 ANTXR1 84168
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
207 PARK2  
Affinity Capture-MS Homo sapiens
208 IGF1R 3480
Affinity Capture-MS Homo sapiens
209 COPE 11316
Affinity Capture-MS Homo sapiens
210 B4GALT2  
Affinity Capture-MS Homo sapiens
211 TUBA1A 7846
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
212 MAPT  
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
213 KIAA1683  
Affinity Capture-MS Homo sapiens
214 TUBA1B 10376
Affinity Capture-MS Homo sapiens
215 BTF3 689
Affinity Capture-MS Homo sapiens
216 DNAJA1 3301
Co-fractionation Homo sapiens
217 CDHR4  
Affinity Capture-MS Homo sapiens
218 C1orf115  
Affinity Capture-MS Homo sapiens
219 PHF12  
Affinity Capture-MS Homo sapiens
220 TMCO3 55002
Affinity Capture-MS Homo sapiens
221 POLR2B 5431
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
222 CBFB 865
Affinity Capture-MS Homo sapiens
223 LRRC23  
Affinity Capture-MS Homo sapiens
224 BACE2 25825
Affinity Capture-MS Homo sapiens
225 TSSC1 7260
Affinity Capture-MS Homo sapiens
226 PRG3  
Affinity Capture-MS Homo sapiens
227 ZHX1-C8orf76  
Affinity Capture-MS Homo sapiens
228 C1QA 712
Affinity Capture-MS Homo sapiens
229 MGARP  
Affinity Capture-MS Homo sapiens
230 PRSS50  
Affinity Capture-MS Homo sapiens
231 MAP2K2 5605
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
232 POLR3E 55718
Affinity Capture-MS Homo sapiens
233 MGRN1 23295
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
234 F12  
Affinity Capture-MS Homo sapiens
235 SERPINF1 5176
Affinity Capture-MS Homo sapiens
236 SLC25A18  
Affinity Capture-MS Homo sapiens
237 SCGB2A2  
Affinity Capture-MS Homo sapiens
238 ENO2 2026
Two-hybrid Homo sapiens
239 SENP5  
Affinity Capture-MS Homo sapiens
240 ISLR 3671
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
241 SIK2  
Affinity Capture-MS Homo sapiens
242 ARAF 369
Affinity Capture-MS Homo sapiens
243 MAGT1 84061
Affinity Capture-MS Homo sapiens
244 TUBB2A 7280
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
245 GALNT10 55568
Affinity Capture-MS Homo sapiens
246 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
247 NSUN5P1  
Affinity Capture-MS Homo sapiens
248 CTDSP1 58190
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
249 AVIL  
Affinity Capture-MS Homo sapiens
250 LIPH 200879
Affinity Capture-MS Homo sapiens
251 TXNDC15  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
252 TUBA4B 80086
Co-fractionation Homo sapiens
253 RPA1 6117
Affinity Capture-MS Homo sapiens
254 TUBB3 10381
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
255 HSPB1 3315
Co-fractionation Homo sapiens
256 CCT7 10574
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
257 ZC2HC1B  
Affinity Capture-MS Homo sapiens
258 C6orf141  
Affinity Capture-MS Homo sapiens
259 PPP4R1L  
Affinity Capture-MS Homo sapiens
260 CDK13 8621
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
261 TBL1Y  
Affinity Capture-MS Homo sapiens
262 METTL3  
Affinity Capture-MS Homo sapiens
263 RPL28 6158
Affinity Capture-MS Homo sapiens
264 SERBP1 26135
Affinity Capture-MS Homo sapiens
265 EDN3  
Affinity Capture-MS Homo sapiens
266 RC3H1 149041
Affinity Capture-MS Homo sapiens
267 ATXN3 4287
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
268 UBE2H 7328
Affinity Capture-MS Homo sapiens
269 INSIG1  
Affinity Capture-MS Homo sapiens
270 PRODH  
Affinity Capture-MS Homo sapiens
271 RAMP3  
Affinity Capture-MS Homo sapiens
272 KLK15  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
273 CCT3 7203
Affinity Capture-MS Homo sapiens
274 CD3D 915
Affinity Capture-MS Homo sapiens
275 SSR2  
Affinity Capture-MS Homo sapiens
276 TNPO1 3842
Co-fractionation Homo sapiens
277 FBXO25  
Affinity Capture-MS Homo sapiens
278 WNT7A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
279 CCT4 10575
Affinity Capture-MS Homo sapiens
280 CD79A  
Affinity Capture-MS Homo sapiens
281 SNX21  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
282 SNX6 58533
Affinity Capture-MS Homo sapiens
283 TOMM22 56993
Affinity Capture-MS Homo sapiens
284 KIAA1429 25962
Affinity Capture-MS Homo sapiens
285 B3GAT1  
Affinity Capture-MS Homo sapiens
286 TBCB 1155
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
287 TGFB1 7040
Affinity Capture-MS Homo sapiens
288 COPS5 10987
Affinity Capture-MS Homo sapiens
289 KIR2DS3  
Affinity Capture-MS Homo sapiens
290 SLC27A2 11001
Affinity Capture-MS Homo sapiens
291 PRPS1 5631
Co-fractionation Homo sapiens
292 SURF1  
Affinity Capture-MS Homo sapiens
293 TUBB4A 10382
Co-fractionation Homo sapiens
294 NFXL1 152518
Affinity Capture-MS Homo sapiens
295 PLA2G10  
Affinity Capture-MS Homo sapiens
296 CEP57  
Affinity Capture-MS Homo sapiens
297 GUSBP5  
Affinity Capture-MS Homo sapiens
298 FAF2 23197
Affinity Capture-MS Homo sapiens
299 FBXW5  
Affinity Capture-MS Homo sapiens
300 DEFB109P1B  
Affinity Capture-MS Homo sapiens
301 DNASE2B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
302 CD80 941
Affinity Capture-MS Homo sapiens
303 HAUS2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
304 DDX39A 10212
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
305 ERBB3 2065
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
306 STAU1 6780
Affinity Capture-MS Homo sapiens
307 PROZ 8858
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
308 VCP 7415
Affinity Capture-MS Homo sapiens
309 MAPT  
Reconstituted Complex Bos taurus
310 BTK 695
Affinity Capture-MS Homo sapiens
311 ARPC5 10092
Affinity Capture-MS Homo sapiens
312 CEP76  
Affinity Capture-MS Homo sapiens
313 LYPD4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
314 DNAJB11 51726
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
315 CAD 790
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
316 FANCD2  
Affinity Capture-MS Homo sapiens
317 CUL3 8452
Affinity Capture-MS Homo sapiens
318 CCT6A 908
Affinity Capture-MS Homo sapiens
319 STXBP1 6812
Affinity Capture-Western Homo sapiens
320 NPTN 27020
Affinity Capture-MS Homo sapiens
321 GPR12  
Affinity Capture-MS Homo sapiens
322 CCP110  
Affinity Capture-MS Homo sapiens
323 TXNRD1 7296
Co-fractionation Homo sapiens
324 GRIN2C 2905
Affinity Capture-MS Homo sapiens
325 CCNJL  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
326 TMEM263 90488
Affinity Capture-MS Homo sapiens
327 NXF1 10482
Affinity Capture-RNA Homo sapiens
328 EPHA7 2045
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
329 C12orf74  
Affinity Capture-MS Homo sapiens
330 DCUN1D1 54165
Affinity Capture-MS Homo sapiens
331 VEPH1 79674
Affinity Capture-MS Homo sapiens
332 P2RY10  
Affinity Capture-MS Homo sapiens
333 TMPRSS5  
Affinity Capture-MS Homo sapiens
334 GBAP1  
Affinity Capture-MS Homo sapiens
335 ARRB2 409
Affinity Capture-MS Homo sapiens
336 SIRT6  
Affinity Capture-MS Homo sapiens
337 HAUS1  
Affinity Capture-MS Homo sapiens
338 DOCK5 80005
Affinity Capture-MS Homo sapiens
339 TUBG1 7283
Affinity Capture-MS Homo sapiens
340 DNAJA2 10294
Affinity Capture-MS Homo sapiens
341 OSTM1 28962
Affinity Capture-MS Homo sapiens
342 CUL5 8065
Affinity Capture-MS Homo sapiens
343 TMEM108  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
344 GZMH  
Affinity Capture-MS Homo sapiens
345 MOV10 4343
Affinity Capture-RNA Homo sapiens
346 SUSD4  
Affinity Capture-MS Homo sapiens
347 ARRB1 408
Affinity Capture-MS Homo sapiens
348 TRIP4 9325
Affinity Capture-MS Homo sapiens
349 ITGA9 3680
Affinity Capture-MS Homo sapiens
350 C9orf72  
Affinity Capture-MS Homo sapiens
351 KBTBD4  
Affinity Capture-MS Homo sapiens
352 C4orf17  
Two-hybrid Homo sapiens
353 TRAV20  
Affinity Capture-MS Homo sapiens
354 LIMK2 3985
Affinity Capture-MS Homo sapiens
355 NEDD8 4738
Affinity Capture-MS Homo sapiens
356 CDC5L 988
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which TUBA4A is involved
PathwayEvidenceSource
Activation of AMPK downstream of NMDARs IEA Reactome
Activation of NMDA receptors and postsynaptic events IEA Reactome
Adaptive Immune System TAS Reactome
Aggrephagy TAS Reactome
Aggrephagy IEA Reactome
Anchoring of the basal body to the plasma membrane TAS Reactome
Antiviral mechanism by IFN-stimulated genes TAS Reactome
Asparagine N-linked glycosylation TAS Reactome
Assembly and cell surface presentation of NMDA receptors IEA Reactome
AURKA Activation by TPX2 TAS Reactome
Autophagy TAS Reactome
Autophagy IEA Reactome
Axon guidance TAS Reactome
Axon guidance IEA Reactome
Carboxyterminal post-translational modifications of tubulin TAS Reactome
Carboxyterminal post-translational modifications of tubulin IEA Reactome
Cell Cycle TAS Reactome
Cell Cycle, Mitotic TAS Reactome
Cellular responses to stimuli TAS Reactome
Cellular responses to stress TAS Reactome
Centrosome maturation TAS Reactome
Chaperonin-mediated protein folding IEA Reactome
Cilium Assembly TAS Reactome
Cooperation of Prefoldin and TriC/CCT in actin and tubulin folding IEA Reactome
COPI-dependent Golgi-to-ER retrograde traffic TAS Reactome
COPI-independent Golgi-to-ER retrograde traffic TAS Reactome
COPI-mediated anterograde transport TAS Reactome
Cytokine Signaling in Immune system TAS Reactome
Developmental Biology TAS Reactome
Developmental Biology IEA Reactome
Disease TAS Reactome
EML4 and NUDC in mitotic spindle formation TAS Reactome
ER to Golgi Anterograde Transport TAS Reactome
Factors involved in megakaryocyte development and platelet production TAS Reactome
Formation of tubulin folding intermediates by CCT/TriC IEA Reactome
G2/M Transition TAS Reactome
Gap junction assembly IEA Reactome
Gap junction assembly TAS Reactome
Gap junction trafficking IEA Reactome
Gap junction trafficking TAS Reactome
Gap junction trafficking and regulation IEA Reactome
Gap junction trafficking and regulation TAS Reactome
Golgi-to-ER retrograde transport TAS Reactome
HCMV Early Events TAS Reactome
HCMV Infection TAS Reactome
Hedgehog 'off' state TAS Reactome
Hemostasis TAS Reactome
HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand TAS Reactome
Immune System TAS Reactome
Infectious disease TAS Reactome
Interferon Signaling TAS Reactome
Intra-Golgi and retrograde Golgi-to-ER traffic TAS Reactome
Intraflagellar transport TAS Reactome
Kinesins TAS Reactome
L1CAM interactions TAS Reactome
L1CAM interactions IEA Reactome
Loss of Nlp from mitotic centrosomes TAS Reactome
Loss of proteins required for interphase microtubule organization from the centrosome TAS Reactome
M Phase TAS Reactome
Macroautophagy TAS Reactome
Macroautophagy IEA Reactome
Membrane Trafficking IEA Reactome
Membrane Trafficking TAS Reactome
Metabolism of proteins IEA Reactome
Metabolism of proteins TAS Reactome
MHC class II antigen presentation TAS Reactome
Microtubule-dependent trafficking of connexons from Golgi to the plasma membrane TAS Reactome
Mitotic Anaphase TAS Reactome
Mitotic G2-G2/M phases TAS Reactome
Mitotic Metaphase and Anaphase TAS Reactome
Mitotic Prometaphase TAS Reactome
Nervous system development TAS Reactome
Nervous system development IEA Reactome
Neuronal System IEA Reactome
Neurotransmitter receptors and postsynaptic signal transmission IEA Reactome
Nuclear Envelope (NE) Reassembly TAS Reactome
Organelle biogenesis and maintenance TAS Reactome
PKR-mediated signaling TAS Reactome
Platelet activation, signaling and aggregation TAS Reactome
Platelet degranulation TAS Reactome
Post NMDA receptor activation events IEA Reactome
Post-chaperonin tubulin folding pathway IEA Reactome
Post-translational protein modification TAS Reactome
Post-translational protein modification IEA Reactome
Prefoldin mediated transfer of substrate to CCT/TriC IEA Reactome
Protein folding IEA Reactome
Recruitment of mitotic centrosome proteins and complexes TAS Reactome
Recruitment of NuMA to mitotic centrosomes TAS Reactome
Recycling pathway of L1 TAS Reactome
Recycling pathway of L1 IEA Reactome
Regulation of PLK1 Activity at G2/M Transition TAS Reactome
Resolution of Sister Chromatid Cohesion TAS Reactome
Response to elevated platelet cytosolic Ca2+ TAS Reactome
RHO GTPase Effectors TAS Reactome
RHO GTPases Activate Formins TAS Reactome
RHO GTPases activate IQGAPs TAS Reactome
Sealing of the nuclear envelope (NE) by ESCRT-III TAS Reactome
Selective autophagy TAS Reactome
Selective autophagy IEA Reactome
Separation of Sister Chromatids TAS Reactome
Signal Transduction TAS Reactome
Signaling by Hedgehog TAS Reactome
Signaling by Rho GTPases TAS Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 TAS Reactome
The role of GTSE1 in G2/M progression after G2 checkpoint TAS Reactome
Translocation of SLC2A4 (GLUT4) to the plasma membrane IEA Reactome
Transmission across Chemical Synapses IEA Reactome
Transport of connexons to the plasma membrane TAS Reactome
Transport to the Golgi and subsequent modification TAS Reactome
Vesicle-mediated transport IEA Reactome
Vesicle-mediated transport TAS Reactome
Viral Infection Pathways TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here