Gene description for KLK6
Gene name kallikrein-related peptidase 6
Gene symbol KLK6
Other names/aliases Bssp
Klk7
PRSS18
PRSS9
SP59
hK6
Species Homo sapiens
 Database cross references - KLK6
ExoCarta ExoCarta_5653
Entrez Gene 5653
HGNC 6367
MIM 602652
UniProt Q92876  
 KLK6 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Hepatocytes 26054723    
 Gene ontology annotations for KLK6
Molecular Function
    protein binding GO:0005515 IPI
    serine-type endopeptidase activity GO:0004252 NAS
Biological Process
    collagen catabolic process GO:0030574 NAS
    hormone metabolic process GO:0042445 NAS
    myelination GO:0042552 NAS
    amyloid precursor protein metabolic process GO:0042982 NAS
    protein processing GO:0016485 IBA
    central nervous system development GO:0007417 NAS
    positive regulation of G-protein coupled receptor protein signaling pathway GO:0045745 IDA
    protein autoprocessing GO:0016540 NAS
    tissue regeneration GO:0042246 NAS
    response to wounding GO:0009611 NAS
    regulation of neuron projection development GO:0010975 IEA
    regulation of cell differentiation GO:0045595 NAS
    neuron death GO:0070997 IMP
Subcellular Localization
    endoplasmic reticulum GO:0005783 IEA
    extracellular space GO:0005615 IDA
    nuclear membrane GO:0031965 IDA
    nucleoplasm GO:0005654 IDA
    microtubule cytoskeleton GO:0015630 IDA
    nucleolus GO:0005730 IEA
    mitochondrion GO:0005739 IEA
    intercellular bridge GO:0045171 IDA
    cytoplasm GO:0005737 IDA
    extracellular region GO:0005576 IDA
 Experiment description of studies that identified KLK6 in exosomes
1
Experiment ID 207
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for KLK6
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which KLK6 is involved
No pathways found





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