Experiment description of studies that identified KLK6 in sEVs

1 Experiment ID 207 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|HSP70|FLOT1 Enriched markers ✔ VDAC Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name DKO-1 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

2 Experiment ID 208 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|HSP70|FLOT1 Enriched markers ✔ VDAC Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name Dks-8 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

3 Experiment ID 209 MISEV standards ✘ Biophysical techniques ✔ TSG101|HSP70|FLOT1 Enriched markers ✔ VDAC Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name DLD-1 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

4 Experiment ID 237 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocytes Sample name MIHA Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RNA Sequencing

Protein-protein interactions for KLK6

Protein Interactor ExoCarta ID Identification method PubMed Species 1 CERS3   Affinity Capture-MS Homo sapiens 2 SLC25A6 293 Affinity Capture-MS Homo sapiens 3 C18orf21   Affinity Capture-MS Homo sapiens 4 TMEM87A 25963 Affinity Capture-MS Homo sapiens 5 AGPAT1 10554 Affinity Capture-MS Homo sapiens 6 NRSN1   Affinity Capture-MS Homo sapiens 7 EMC7 56851 Affinity Capture-MS Homo sapiens 8 TUBB3 10381 Affinity Capture-MS Homo sapiens 9 FBXO42   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 10 RNF115   Affinity Capture-MS Homo sapiens 11 TUBA4A 7277 Affinity Capture-MS Homo sapiens 12 CDYL 9425 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 13 SARAF   Affinity Capture-MS Homo sapiens 14 PIGT 51604 Affinity Capture-MS Homo sapiens 15 PINK1   Affinity Capture-MS Homo sapiens 16 CPSF4   Affinity Capture-MS Homo sapiens 17 SSUH2   Affinity Capture-MS Homo sapiens 18 OR2A4 79541 Affinity Capture-MS Homo sapiens 19 GABPA 2551 Affinity Capture-MS Homo sapiens 20 CCR1   Affinity Capture-MS Homo sapiens 21 STRBP 55342 Affinity Capture-MS Homo sapiens 22 UBE2U   Affinity Capture-MS Homo sapiens 23 PHF11 51131 Affinity Capture-MS Homo sapiens View the network

Pathways in which KLK6 is involved

No pathways found