Gene description for POLR2B
Gene name polymerase (RNA) II (DNA directed) polypeptide B, 140kDa
Gene symbol POLR2B
Other names/aliases POL2RB
RPB2
hRPB140
Species Homo sapiens
 Database cross references - POLR2B
ExoCarta ExoCarta_5431
Vesiclepedia VP_5431
Entrez Gene 5431
HGNC 9188
MIM 180661
UniProt P30876  
 POLR2B identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 36706192    
Embryonic kidney cells 36706192    
Foreskin fibroblasts 34108659    
Lymphoma cells 34108659    
Mammary cancer-associated fibroblasts 34108659    
Mesenchymal stem cells 36408942    
Monocytic leukemia cells 34108659    
Normal mammary epithelial cells 34108659    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic duct epithalial cells 34108659    
Pancreatic duct epithalial cells 34108659    
Pluripotent stem cells 34108659    
T lymphocytes 34108659    
Thymus 23844026    
 Gene ontology annotations for POLR2B
Molecular Function
    RNA polymerase I activity GO:0001054 IEA
    RNA polymerase II activity GO:0001055 IBA
    RNA polymerase II activity GO:0001055 ISS
    RNA polymerase III activity GO:0001056 IEA
    RNA polymerase IV activity GO:0001057 IEA
    RNA polymerase V activity GO:0001058 IEA
    mitochondrial single subunit type RNA polymerase activity GO:0001065 IEA
    plastid single subunit type RNA polymerase activity GO:0001066 IEA
    DNA binding GO:0003677 IEA
    chromatin binding GO:0003682 IEA
    RNA binding GO:0003723 HDA
    DNA-directed 5'-3' RNA polymerase activity GO:0003899 IDA
    RNA-dependent RNA polymerase activity GO:0003968 IDA
    protein binding GO:0005515 IPI
    hydrolase activity GO:0016787 IEA
    ribonucleoside binding GO:0032549 IEA
    metal ion binding GO:0046872 IEA
Biological Process
    transcription by RNA polymerase IV GO:0001059 IEA
    transcription by RNA polymerase V GO:0001060 IEA
    RNA-templated transcription GO:0001172 IEA
    transcription by RNA polymerase I GO:0006360 IEA
    transcription by RNA polymerase II GO:0006366 IDA
    transcription by RNA polymerase III GO:0006383 IEA
    mitochondrial transcription GO:0006390 IEA
    plastid transcription GO:0042793 IEA
Subcellular Localization
    chromosome, telomeric region GO:0000781 IDA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    RNA polymerase II, core complex GO:0005665 IBA
    RNA polymerase II, core complex GO:0005665 IDA
    membrane GO:0016020 HDA
 Experiment description of studies that identified POLR2B in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 412
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MCF7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
6
Experiment ID 414
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
7
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
10
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
11
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
13
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
14
Experiment ID 407
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|TSG101|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
15
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
16
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
17
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
18
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
19
Experiment ID 1275
MISEV standards
Biophysical techniques
✔
CD9|TSG101|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
Enriched markers
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
20
Experiment ID 1280
MISEV standards
Biophysical techniques
✔
TSG101|CD9|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
Enriched markers
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T - Fraction 5
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density 1.151 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
21
Experiment ID 405
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Foreskin fibroblasts
Sample name BJ
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
22
Experiment ID 417
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Lymphoma cells
Sample name Raji
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
23
Experiment ID 411
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Mammary cancer-associated fibroblasts
Sample name mCAF
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
24
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
25
Experiment ID 418
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Monocytic leukemia cells
Sample name THP-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
26
Experiment ID 418
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Monocytic leukemia cells
Sample name THP-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
27
Experiment ID 413
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Normal mammary epithelial cells
Sample name MCF10A
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
28
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
29
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
30
Experiment ID 406
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35|CD81
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name BxPC3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
31
Experiment ID 415
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
32
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
33
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
34
Experiment ID 408
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPDE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
35
Experiment ID 409
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPNE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
36
Experiment ID 416
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pluripotent stem cells
Sample name PSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
37
Experiment ID 410
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample T lymphocytes
Sample name Jurkat
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
38
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for POLR2B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 POLR2G 5436
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
3 SNRPA 6626
Affinity Capture-MS Homo sapiens
4 HIST2H2BE 8349
Affinity Capture-MS Homo sapiens
5 RIT1 6016
Negative Genetic Homo sapiens
6 GTF2F1 2962
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
7 OTUD5 55593
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
8 VWA9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 RNGTT 8732
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 SUPT6H 6830
Affinity Capture-MS Homo sapiens
11 KPNA3 3839
Affinity Capture-MS Homo sapiens
12 CCNH  
Biochemical Activity Homo sapiens
13 MED17  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 MED20 9477
Affinity Capture-MS Homo sapiens
15 Ruvbl1 56505
Affinity Capture-MS Mus musculus
16 CRTAP 10491
Co-fractionation Homo sapiens
17 ATP6V1B2 526
Co-fractionation Homo sapiens
18 WWP1 11059
Affinity Capture-MS Homo sapiens
19 SMURF1 57154
Affinity Capture-MS Homo sapiens
20 RABEP2 79874
Co-fractionation Homo sapiens
21 H1FX 8971
Co-fractionation Homo sapiens
22 MED6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 BTF3 689
Reconstituted Complex Homo sapiens
24 ZNHIT2 741
Affinity Capture-MS Homo sapiens
25 MED29  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 SUB1 10923
Reconstituted Complex Homo sapiens
27 INTS10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 MED9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 MED13L 23389
Affinity Capture-MS Homo sapiens
30 INTS2  
Affinity Capture-MS Homo sapiens
31 C9orf78 51759
Affinity Capture-MS Homo sapiens
32 NABP2  
Affinity Capture-MS Homo sapiens
33 BRD2  
Affinity Capture-MS Homo sapiens
34 C7orf76  
Affinity Capture-MS Homo sapiens
35 FAM168A  
Affinity Capture-MS Homo sapiens
36 XRN2 22803
Affinity Capture-MS Homo sapiens
37 CALM1 801
Reconstituted Complex Homo sapiens
38 CAND1 55832
Affinity Capture-MS Homo sapiens
39 AAR2 25980
Affinity Capture-MS Homo sapiens
40 INTS1 26173
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 SYDE2  
Affinity Capture-MS Homo sapiens
42 ASXL1  
Affinity Capture-MS Homo sapiens
43 CDK8 1024
Affinity Capture-MS Homo sapiens
44 URI1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 GTF2E1 2960
Co-fractionation Homo sapiens
46 TCEA1 6917
Affinity Capture-MS Homo sapiens
47 TCEB3 6924
Co-localization Homo sapiens
Affinity Capture-MS Homo sapiens
Co-localization Homo sapiens
48 LMO2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 PARK2  
Affinity Capture-MS Homo sapiens
50 MED13 9969
Affinity Capture-MS Homo sapiens
51 RUVBL1 8607
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 WDR76  
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
53 NCBP1 4686
Affinity Capture-MS Homo sapiens
54 GTF2F2 2963
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
55 DCTN4 51164
Proximity Label-MS Homo sapiens
56 INIP  
Affinity Capture-MS Homo sapiens
57 POLR2I 5438
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
58 MRPS36 92259
Affinity Capture-MS Homo sapiens
59 FBXW7  
Affinity Capture-MS Homo sapiens
60 SRC 6714
Co-localization Homo sapiens
Co-localization Homo sapiens
61 MDC1  
Affinity Capture-MS Homo sapiens
62 SNRPC 6631
Affinity Capture-MS Homo sapiens
63 MED27  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 CLIP1 6249
Co-fractionation Homo sapiens
65 OBSL1 23363
Affinity Capture-MS Homo sapiens
66 CRCP  
Affinity Capture-MS Homo sapiens
67 FANCD2  
Affinity Capture-MS Homo sapiens
68 MED22  
Affinity Capture-MS Homo sapiens
69 INTS6 26512
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 PSMB9 5698
Co-localization Homo sapiens
Co-localization Homo sapiens
71 ATG16L1 55054
Affinity Capture-MS Homo sapiens
72 POLR2C 5432
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
73 GPN1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
74 POLR1E  
Affinity Capture-MS Homo sapiens
75 POLR2M  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 MYCN  
Affinity Capture-MS Homo sapiens
77 ASNSD1  
Affinity Capture-MS Homo sapiens
78 HNRNPD 3184
Affinity Capture-MS Homo sapiens
79 PFDN6 10471
Affinity Capture-MS Homo sapiens
80 CCNC  
Affinity Capture-MS Homo sapiens
81 NEDD4 4734
Reconstituted Complex Homo sapiens
82 EEF1A1 1915
Co-fractionation Homo sapiens
83 KIF14 9928
Affinity Capture-MS Homo sapiens
84 MED4 29079
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
85 GPN3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
86 POLR2K  
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
87 MED1 5469
Affinity Capture-MS Homo sapiens
88 POLR1A 25885
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
89 INTS12  
Affinity Capture-MS Homo sapiens
90 BAG5 9529
Affinity Capture-MS Homo sapiens
91 NUMA1 4926
Affinity Capture-MS Homo sapiens
92 RBBP5 5929
Co-fractionation Homo sapiens
93 CPNE2 221184
Affinity Capture-MS Homo sapiens
94 SCAF1  
Affinity Capture-MS Homo sapiens
95 SLTM 79811
Co-fractionation Homo sapiens
96 MED7  
Affinity Capture-MS Homo sapiens
97 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
98 UXT 8409
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
99 CTDP1 9150
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
100 WWP2 11060
Affinity Capture-MS Homo sapiens
101 SCAF4 57466
Affinity Capture-MS Homo sapiens
102 SUPT5H 6829
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
103 MED18  
Affinity Capture-MS Homo sapiens
104 PALM3  
Affinity Capture-MS Homo sapiens
105 PSMD14 10213
Affinity Capture-MS Homo sapiens
106 INTS4  
Affinity Capture-MS Homo sapiens
107 POLR3F  
Co-fractionation Homo sapiens
108 SNRNP200 23020
Co-fractionation Homo sapiens
109 C12orf49  
Negative Genetic Homo sapiens
110 PFDN2 5202
Affinity Capture-MS Homo sapiens
111 TUBB 203068
Co-fractionation Homo sapiens
112 NELFE 7936
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
113 Gtf2b  
Affinity Capture-MS Mus musculus
114 VCP 7415
Affinity Capture-MS Homo sapiens
115 NTRK1 4914
Affinity Capture-MS Homo sapiens
116 MED26  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
117 DCTN1 1639
Co-fractionation Homo sapiens
118 FBXL6  
Affinity Capture-MS Homo sapiens
119 GTF2IRD2B  
Affinity Capture-MS Homo sapiens
120 Bag2  
Affinity Capture-MS Mus musculus
121 HECTD1 25831
Affinity Capture-MS Homo sapiens
122 CTR9 9646
Affinity Capture-MS Homo sapiens
123 POLE  
Affinity Capture-MS Homo sapiens
124 MED31  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
125 ITCH 83737
Reconstituted Complex Homo sapiens
126 PRKACB 5567
Affinity Capture-MS Homo sapiens
127 KPNA4 3840
Affinity Capture-MS Homo sapiens
128 BARD1 580
Affinity Capture-MS Homo sapiens
129 CD3EAP  
Affinity Capture-MS Homo sapiens
130 DNAJC7 7266
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
131 REEP5 7905
Co-fractionation Homo sapiens
132 WDR82 80335
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
133 DDX26B  
Affinity Capture-MS Homo sapiens
134 RECQL5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
135 C9orf72  
Affinity Capture-MS Homo sapiens
136 RBM39 9584
Affinity Capture-MS Homo sapiens
137 CDK7 1022
Biochemical Activity Homo sapiens
138 SNAR-B2  
Reconstituted Complex Homo sapiens
139 POLR3A 11128
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
140 POLR2H 5437
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
141 XPO1 7514
Affinity Capture-MS Homo sapiens
142 EIF6 3692
Proximity Label-MS Homo sapiens
143 NELFCD 51497
Affinity Capture-MS Homo sapiens
144 SNRPB 6628
Affinity Capture-MS Homo sapiens
145 ZSCAN1  
Two-hybrid Homo sapiens
146 POLR2E 5434
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
147 TUBB4B 10383
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
148 INTS7  
Affinity Capture-MS Homo sapiens
149 ASB6 140459
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
150 RPRD2  
Affinity Capture-MS Homo sapiens
151 CDK19 23097
Affinity Capture-MS Homo sapiens
152 CUL3 8452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
153 MECP2 4204
Affinity Capture-MS Homo sapiens
154 LZTR1 8216
Two-hybrid Homo sapiens
155 POLR2D  
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
156 FBXO6 26270
Affinity Capture-MS Homo sapiens
157 FUS 2521
Affinity Capture-MS Homo sapiens
158 METTL3  
Affinity Capture-MS Homo sapiens
159 POLR2A 5430
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
160 SUZ12  
Affinity Capture-MS Homo sapiens
161 POLR3E 55718
Affinity Capture-MS Homo sapiens
162 PPP2CA 5515
Affinity Capture-MS Homo sapiens
163 TBP  
Affinity Capture-MS Homo sapiens
164 TUBA4A 7277
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
165 PRMT5 10419
Co-fractionation Homo sapiens
166 FKBP5 2289
Affinity Capture-MS Homo sapiens
167 MAPK6  
Affinity Capture-MS Homo sapiens
168 GPN2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
169 TANGO6 79613
Affinity Capture-MS Homo sapiens
170 TADA2A  
Affinity Capture-MS Homo sapiens
171 MED24 9862
Affinity Capture-MS Homo sapiens
172 MYC  
Affinity Capture-MS Homo sapiens
173 HECW2  
Affinity Capture-MS Homo sapiens
174 INO80B 83444
Affinity Capture-MS Homo sapiens
175 ZNRD1  
Affinity Capture-MS Homo sapiens
176 WDR5 11091
Co-fractionation Homo sapiens
177 TOP1 7150
Affinity Capture-MS Homo sapiens
178 EEFSEC 60678
Co-fractionation Homo sapiens
179 RPAP3 79657
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
180 CDK20  
Affinity Capture-MS Homo sapiens
181 BICD1 636
Proximity Label-MS Homo sapiens
182 DHFRL1  
Proximity Label-MS Homo sapiens
183 RNF2  
Affinity Capture-MS Homo sapiens
184 ILK 3611
Affinity Capture-MS Homo sapiens
185 HAX1  
Proximity Label-MS Homo sapiens
186 SNRPF 6636
Affinity Capture-MS Homo sapiens
187 NELFB 25920
Affinity Capture-MS Homo sapiens
188 CPSF3L 54973
Affinity Capture-MS Homo sapiens
189 INTS5 80789
Affinity Capture-MS Homo sapiens
190 RIPK4  
Affinity Capture-MS Homo sapiens
191 AKAP1 8165
Proximity Label-MS Homo sapiens
192 MED14  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
193 FLOT1 10211
Proximity Label-MS Homo sapiens
194 HUWE1 10075
Affinity Capture-MS Homo sapiens
195 INTS9 55756
Affinity Capture-MS Homo sapiens
196 HIF1A 3091
Affinity Capture-MS Homo sapiens
197 MED11  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
198 C7orf26  
Affinity Capture-MS Homo sapiens
199 MED19  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
200 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
201 POLR2F  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
202 SIRT6  
Affinity Capture-MS Homo sapiens
203 CEP170P1  
Affinity Capture-MS Homo sapiens
204 WDR77 79084
Co-fractionation Homo sapiens
205 POLR2J  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
206 TP53 7157
Affinity Capture-MS Homo sapiens
207 POLR1B 84172
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
208 GCOM1  
Affinity Capture-MS Homo sapiens
209 RUVBL2 10856
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
210 POLR2L 5441
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
211 SAFB2 9667
Co-fractionation Homo sapiens
212 TRIM52  
Affinity Capture-MS Homo sapiens
213 UVSSA  
Affinity Capture-MS Homo sapiens
214 POLR1C 9533
Affinity Capture-MS Homo sapiens
215 WDR92 116143
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
216 POLR3H  
Affinity Capture-MS Homo sapiens
217 MEN1 4221
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
218 RPAP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
219 CUL5 8065
Affinity Capture-MS Homo sapiens
220 KIAA1429 25962
Affinity Capture-MS Homo sapiens
221 HMGN5 79366
Affinity Capture-MS Homo sapiens
222 MED8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
223 PIDD1  
Affinity Capture-MS Homo sapiens
224 RPAP2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
225 INTS8  
Affinity Capture-MS Homo sapiens
226 SHFM1 7979
Affinity Capture-MS Homo sapiens
227 NELFA  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
228 WWOX 51741
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
229 EPB41L2 2037
Co-fractionation Homo sapiens
230 RPS5 6193
Co-fractionation Homo sapiens
231 GTF2B 2959
Affinity Capture-MS Homo sapiens
232 ESR1  
Co-localization Homo sapiens
Co-localization Homo sapiens
233 ANAPC2 29882
Proximity Label-MS Homo sapiens
234 UFL1 23376
Affinity Capture-MS Homo sapiens
235 MED16 10025
Affinity Capture-MS Homo sapiens
236 ERCC3  
Co-fractionation Homo sapiens
237 MED10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
238 ERCC6  
Affinity Capture-MS Homo sapiens
239 MED21  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
240 KDM1A 23028
Affinity Capture-MS Homo sapiens
241 MED12  
Affinity Capture-MS Homo sapiens
242 NCL 4691
Affinity Capture-MS Homo sapiens
243 Rprd1b  
Affinity Capture-MS Mus musculus
244 PSMA5 5686
Affinity Capture-MS Homo sapiens
245 PHRF1  
Affinity Capture-MS Homo sapiens
246 AIRE  
Affinity Capture-MS Homo sapiens
247 POLR3D  
Affinity Capture-MS Homo sapiens
248 MED30  
Affinity Capture-MS Homo sapiens
249 CIT 11113
Affinity Capture-MS Homo sapiens
250 POLR3B 55703
Affinity Capture-MS Homo sapiens
251 RPRD1B 58490
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
252 BRD1 23774
Affinity Capture-MS Homo sapiens
253 ASH2L 9070
Co-fractionation Homo sapiens
254 PIH1D1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
255 INTS3 65123
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
256 PTGES3 10728
Affinity Capture-MS Homo sapiens
257 KRAS 3845
Synthetic Lethality Homo sapiens
Negative Genetic Homo sapiens
258 POLR1D 51082
Affinity Capture-MS Homo sapiens
259 ZC3H13 23091
Affinity Capture-MS Homo sapiens
260 KBTBD4  
Affinity Capture-MS Homo sapiens
261 PPP2R1A 5518
Affinity Capture-MS Homo sapiens
262 SSRP1 6749
Affinity Capture-MS Homo sapiens
263 RPA3 6119
Proximity Label-MS Homo sapiens
264 CPSF6 11052
Affinity Capture-MS Homo sapiens
265 PDRG1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
266 RPRD1A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
267 CUL4A 8451
Affinity Capture-MS Homo sapiens
268 IKBKG 8517
Two-hybrid Homo sapiens
269 MED28  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
270 GTF2E2  
Co-fractionation Homo sapiens
View the network image/svg+xml
 Pathways in which POLR2B is involved
PathwayEvidenceSource
Abortive elongation of HIV-1 transcript in the absence of Tat IEA Reactome
Activation of anterior HOX genes in hindbrain development during early embryogenesis IEA Reactome
Activation of HOX genes during differentiation IEA Reactome
Cell Cycle TAS Reactome
Chromosome Maintenance TAS Reactome
Developmental Biology IEA Reactome
Disease IEA Reactome
Disease TAS Reactome
Diseases of signal transduction by growth factor receptors and second messengers IEA Reactome
DNA Repair TAS Reactome
Dual incision in TC-NER TAS Reactome
ESR-mediated signaling TAS Reactome
Estrogen-dependent gene expression TAS Reactome
FGFR2 alternative splicing TAS Reactome
FGFR2 mutant receptor activation IEA Reactome
Formation of HIV elongation complex in the absence of HIV Tat IEA Reactome
Formation of HIV-1 elongation complex containing HIV-1 Tat TAS Reactome
Formation of HIV-1 elongation complex containing HIV-1 Tat IEA Reactome
Formation of RNA Pol II elongation complex TAS Reactome
Formation of TC-NER Pre-Incision Complex TAS Reactome
Formation of the Early Elongation Complex TAS Reactome
Formation of the HIV-1 Early Elongation Complex IEA Reactome
Gap-filling DNA repair synthesis and ligation in TC-NER TAS Reactome
Gene expression (Transcription) IEA Reactome
Gene expression (Transcription) TAS Reactome
Gene Silencing by RNA IEA Reactome
Gene Silencing by RNA TAS Reactome
Generic Transcription Pathway TAS Reactome
HIV elongation arrest and recovery IEA Reactome
HIV Infection IEA Reactome
HIV Infection TAS Reactome
HIV Life Cycle IEA Reactome
HIV Life Cycle TAS Reactome
HIV Transcription Elongation TAS Reactome
HIV Transcription Elongation IEA Reactome
HIV Transcription Initiation IEA Reactome
Infectious disease IEA Reactome
Infectious disease TAS Reactome
Influenza Infection TAS Reactome
Influenza Viral RNA Transcription and Replication TAS Reactome
Inhibition of DNA recombination at telomere TAS Reactome
Late Phase of HIV Life Cycle IEA Reactome
Late Phase of HIV Life Cycle TAS Reactome
Metabolism of RNA TAS Reactome
MicroRNA (miRNA) biogenesis TAS Reactome
mRNA Capping TAS Reactome
mRNA Splicing TAS Reactome
mRNA Splicing - Major Pathway TAS Reactome
mRNA Splicing - Minor Pathway TAS Reactome
Nucleotide Excision Repair TAS Reactome
Pausing and recovery of HIV elongation IEA Reactome
Pausing and recovery of Tat-mediated HIV elongation IEA Reactome
PIWI-interacting RNA (piRNA) biogenesis IEA Reactome
Processing of Capped Intron-Containing Pre-mRNA TAS Reactome
RNA Pol II CTD phosphorylation and interaction with CE TAS Reactome
RNA Pol II CTD phosphorylation and interaction with CE during HIV infection IEA Reactome
RNA Polymerase II HIV Promoter Escape IEA Reactome
RNA Polymerase II Pre-transcription Events TAS Reactome
RNA Polymerase II Promoter Escape TAS Reactome
RNA polymerase II transcribes snRNA genes TAS Reactome
RNA polymerase II transcribes snRNA genes IEA Reactome
RNA Polymerase II Transcription TAS Reactome
RNA Polymerase II Transcription IEA Reactome
RNA Polymerase II Transcription Elongation TAS Reactome
RNA Polymerase II Transcription Initiation TAS Reactome
RNA Polymerase II Transcription Initiation And Promoter Clearance TAS Reactome
RNA Polymerase II Transcription Pre-Initiation And Promoter Opening TAS Reactome
Signal Transduction TAS Reactome
Signaling by FGFR TAS Reactome
Signaling by FGFR in disease IEA Reactome
Signaling by FGFR2 TAS Reactome
Signaling by FGFR2 IIIa TM IEA Reactome
Signaling by FGFR2 in disease IEA Reactome
Signaling by Nuclear Receptors TAS Reactome
Signaling by Receptor Tyrosine Kinases TAS Reactome
Tat-mediated elongation of the HIV-1 transcript TAS Reactome
Tat-mediated elongation of the HIV-1 transcript IEA Reactome
Tat-mediated HIV elongation arrest and recovery IEA Reactome
Telomere Maintenance TAS Reactome
TP53 Regulates Transcription of DNA Repair Genes TAS Reactome
Transcription of the HIV genome IEA Reactome
Transcription of the HIV genome TAS Reactome
Transcription-Coupled Nucleotide Excision Repair (TC-NER) TAS Reactome
Transcriptional regulation by small RNAs TAS Reactome
Transcriptional Regulation by TP53 TAS Reactome
Viral Infection Pathways IEA Reactome
Viral Infection Pathways TAS Reactome
Viral Messenger RNA Synthesis TAS Reactome





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