Gene description for MED13L
Gene name mediator complex subunit 13-like
Gene symbol MED13L
Other names/aliases PROSIT240
THRAP2
TRAP240L
Species Homo sapiens
 Database cross references - MED13L
ExoCarta ExoCarta_23389
Entrez Gene 23389
HGNC 22962
MIM 608771
UniProt Q71F56  
 MED13L identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
Hepatocytes 26054723    
 Gene ontology annotations for MED13L
Molecular Function
    RNA polymerase II transcription cofactor activity GO:0001104 IEA
Biological Process
    transcription from RNA polymerase II promoter GO:0006366 IEA
    regulation of transcription from RNA polymerase II promoter GO:0006357 IEA
Subcellular Localization
    mediator complex GO:0016592 IEA
 Experiment description of studies that identified MED13L in exosomes
1
Experiment ID 226
ISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH
EV Cytosolic markers
✔
CD9|FLOT1
EV Membrane markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
✘ EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
2
Experiment ID 237
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for MED13L
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MED9  
Affinity Capture-MS Homo sapiens
2 TRG20  
Affinity Capture-MS Homo sapiens
3 IXL  
Affinity Capture-MS Homo sapiens
4 CRSP7  
Affinity Capture-MS Homo sapiens
5 MED19  
Affinity Capture-MS Homo sapiens
6 MED28  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which MED13L is involved
PathwayEvidenceSource
PPARA activates gene expression TAS Reactome
Transcriptional regulation of white adipocyte differentiation TAS Reactome





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