Gene description for MED13L
Gene name mediator complex subunit 13-like
Gene symbol MED13L
Other names/aliases PROSIT240
THRAP2
TRAP240L
Species Homo sapiens
 Database cross references - MED13L
ExoCarta ExoCarta_23389
Vesiclepedia VP_23389
Entrez Gene 23389
HGNC 22962
MIM 608771
UniProt Q71F56  
 MED13L identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
Hepatocytes 26054723    
 Gene ontology annotations for MED13L
Molecular Function
    transcription coregulator activity GO:0003712 IEA
Biological Process
    regulation of transcription by RNA polymerase II GO:0006357 IEA
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    mediator complex GO:0016592 IEA
 Experiment description of studies that identified MED13L in exosomes
1
Experiment ID 226
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD9|FLOT1
Enriched markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
✘ Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
2
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for MED13L
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 POLR2B 5431
Affinity Capture-MS Homo sapiens
2 MED11  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 POLR2C 5432
Affinity Capture-MS Homo sapiens
4 UBE2H 7328
Affinity Capture-MS Homo sapiens
5 MED19  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 POLR2G 5436
Affinity Capture-MS Homo sapiens
7 POLR2H 5437
Affinity Capture-MS Homo sapiens
8 MED8  
Affinity Capture-MS Homo sapiens
9 MED15  
Affinity Capture-MS Homo sapiens
10 MED17  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 MED20 9477
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 CCNC  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 CDK8 1024
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 MED4 29079
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 MED1 5469
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 RPAP2  
Affinity Capture-MS Homo sapiens
17 MED29  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 POLR2E 5434
Affinity Capture-MS Homo sapiens
19 ACAD11 84129
Affinity Capture-MS Homo sapiens
20 FBXL19  
Affinity Capture-MS Homo sapiens
21 MED9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 CDK19 23097
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 MED7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 MED6  
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
25 Med22  
Affinity Capture-MS Mus musculus
26 POLR2I 5438
Affinity Capture-MS Homo sapiens
27 MED24 9862
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 POLR2A 5430
Affinity Capture-MS Homo sapiens
29 SOX2  
Affinity Capture-MS Homo sapiens
30 MED12L  
Affinity Capture-MS Homo sapiens
31 MED30  
Affinity Capture-MS Homo sapiens
32 MED16 10025
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
33 SGTB  
Affinity Capture-MS Homo sapiens
34 MED10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
35 MED18  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 MED21  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 C12orf49  
Positive Genetic Homo sapiens
38 MED12  
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 NPAS1  
Affinity Capture-MS Homo sapiens
40 SORT1 6272
Affinity Capture-MS Homo sapiens
41 MED26  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 SYNCRIP 10492
Affinity Capture-MS Homo sapiens
43 FBXW7  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 MED23 9439
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 ATAD3B 83858
Affinity Capture-MS Homo sapiens
46 AR 367
Proximity Label-MS Homo sapiens
47 MED27  
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
48 MED31  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 MED13 9969
Affinity Capture-MS Homo sapiens
50 CEBPA  
Proximity Label-MS Homo sapiens
51 MED14  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 MED22  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 MED28  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 HIF1A 3091
Affinity Capture-Western Homo sapiens
View the network image/svg+xml



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