Gene description for MED13
Gene name mediator complex subunit 13
Gene symbol MED13
Other names/aliases ARC250
DRIP250
HSPC221
THRAP1
TRAP240
Species Homo sapiens
 Database cross references - MED13
ExoCarta ExoCarta_9969
Entrez Gene 9969
HGNC 22474
MIM 603808
UniProt Q9UHV7  
 MED13 identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
Hepatocytes 26054723    
 Gene ontology annotations for MED13
Molecular Function
    transcription coactivator activity GO:0003713 IDA
    receptor activity GO:0004872 IDA
    RNA polymerase II transcription cofactor activity GO:0001104 IDA
    ligand-dependent nuclear receptor transcription coactivator activity GO:0030374 NAS
    thyroid hormone receptor binding GO:0046966 IDA
    vitamin D receptor binding GO:0042809 NAS
    transcription cofactor activity GO:0003712 IDA
Biological Process
    intracellular steroid hormone receptor signaling pathway GO:0030518 IDA
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IDA
    transcription initiation from RNA polymerase II promoter GO:0006367 TAS
    positive regulation of transcription, DNA-templated GO:0045893 IDA
    androgen receptor signaling pathway GO:0030521 IDA
    gene expression GO:0010467 TAS
Subcellular Localization
    membrane GO:0016020 IDA
    nucleoplasm GO:0005654 TAS
    mediator complex GO:0016592 IDA
    nucleus GO:0005634 IDA
 Experiment description of studies that identified MED13 in exosomes
1
Experiment ID 226
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD9|FLOT1
EV Membrane markers
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
2
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for MED13
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MED9  
Affinity Capture-MS Homo sapiens
2 BRCA1 672
Affinity Capture-MS Homo sapiens
3 VDR  
Affinity Capture-MS Homo sapiens
4 TRG20  
Affinity Capture-MS Homo sapiens
5 CDK8  
Affinity Capture-MS Homo sapiens
6 IXL  
Affinity Capture-MS Homo sapiens
7 CRSP7  
Affinity Capture-MS Homo sapiens
8 MED19  
Affinity Capture-MS Homo sapiens
9 MED28  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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