Gene description for MED16
Gene name mediator complex subunit 16
Gene symbol MED16
Other names/aliases DRIP92
THRAP5
TRAP95
Species Homo sapiens
 Database cross references - MED16
ExoCarta ExoCarta_10025
Vesiclepedia VP_10025
Entrez Gene 10025
HGNC 17556
MIM 604062
UniProt Q9Y2X0  
 MED16 identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
 Gene ontology annotations for MED16
Molecular Function
    receptor activity GO:0004872 IDA
    transcription coactivator activity GO:0003713 TAS
    thyroid hormone receptor binding GO:0046966 IDA
    transcription cofactor activity GO:0003712 IDA
    catalytic activity GO:0003824 IEA
    thyroid hormone receptor coactivator activity GO:0030375 IDA
    vitamin D receptor binding GO:0042809 NAS
    protein binding GO:0005515 IPI
Biological Process
    positive regulation of transcription, DNA-templated GO:0045893 IDA
    transcription initiation from RNA polymerase II promoter GO:0006367 TAS
    transcription from RNA polymerase II promoter GO:0006366 TAS
    intracellular steroid hormone receptor signaling pathway GO:0030518 IDA
    androgen receptor signaling pathway GO:0030521 IDA
    positive regulation of receptor activity GO:2000273 IDA
    gene expression GO:0010467 TAS
    regulation of transcription from RNA polymerase II promoter GO:0006357 NAS
Subcellular Localization
    membrane GO:0016020 IDA
    nucleoplasm GO:0005654 TAS
    nucleus GO:0005634 NAS
    mediator complex GO:0016592 IDA
 Experiment description of studies that identified MED16 in exosomes
1
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for MED16
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 THRA  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
2 MED9  
Affinity Capture-MS Homo sapiens
3 VDR  
Affinity Capture-MS Homo sapiens
4 TRG20  
Affinity Capture-MS Homo sapiens
5 PPARGC1A  
Affinity Capture-Western Homo sapiens
6 IXL  
Affinity Capture-MS Homo sapiens
7 CDK8 1024
Affinity Capture-MS Homo sapiens
8 CRSP7  
Affinity Capture-MS Homo sapiens
9 MED19  
Affinity Capture-MS Homo sapiens
10 MED28  
Affinity Capture-MS Homo sapiens
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