Gene description for MED16
Gene name mediator complex subunit 16
Gene symbol MED16
Other names/aliases DRIP92
THRAP5
TRAP95
Species Homo sapiens
 Database cross references - MED16
ExoCarta ExoCarta_10025
Vesiclepedia VP_10025
Entrez Gene 10025
HGNC 17556
MIM 604062
UniProt Q9Y2X0  
 MED16 identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
 Gene ontology annotations for MED16
Molecular Function
    transcription coactivator activity GO:0003713 IDA
    protein binding GO:0005515 IPI
    nuclear vitamin D receptor binding GO:0042809 NAS
    nuclear thyroid hormone receptor binding GO:0046966 IDA
Biological Process
    regulation of transcription by RNA polymerase II GO:0006357 NAS
    transcription by RNA polymerase II GO:0006366 TAS
    positive regulation of transcription elongation by RNA polymerase II GO:0032968 NAS
    positive regulation of DNA-templated transcription GO:0045893 IBA
    positive regulation of DNA-templated transcription GO:0045893 IDA
    RNA polymerase II preinitiation complex assembly GO:0051123 NAS
    positive regulation of transcription initiation by RNA polymerase II GO:0060261 IDA
    positive regulation of transcription initiation by RNA polymerase II GO:0060261 NAS
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleus GO:0005634 NAS
    nucleoplasm GO:0005654 TAS
    membrane GO:0016020 HDA
    mediator complex GO:0016592 IBA
    mediator complex GO:0016592 IDA
    core mediator complex GO:0070847 IPI
 Experiment description of studies that identified MED16 in sEVs
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for MED16
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 POLR2G 5436
Affinity Capture-MS Homo sapiens
3 RALY 22913
Co-fractionation Homo sapiens
4 MED17  
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 MED7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 CIB2  
Affinity Capture-MS Homo sapiens
7 TRRAP 8295
Co-purification Homo sapiens
8 MED29  
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
9 QKI 9444
Affinity Capture-MS Homo sapiens
10 FBXL19  
Affinity Capture-MS Homo sapiens
11 MED9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
12 MED13L 23389
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
13 MED24 9862
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
14 Med22  
Affinity Capture-MS Mus musculus
15 MED12L  
Affinity Capture-MS Homo sapiens
16 MED13 9969
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
17 FBXW7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 MED27  
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 MED22  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 TACSTD2 4070
Affinity Capture-MS Homo sapiens
21 BRD4 23476
Affinity Capture-MS Homo sapiens
22 DNAJC7 7266
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 ATG16L1 55054
Affinity Capture-MS Homo sapiens
24 XRCC3  
Affinity Capture-MS Homo sapiens
25 MECP2 4204
Affinity Capture-MS Homo sapiens
26 VDR  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 MED4 29079
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
28 MED1 5469
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
29 AURKA 6790
Affinity Capture-MS Homo sapiens
30 ATAD3B 83858
Affinity Capture-MS Homo sapiens
31 MED20 9477
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 CTDP1 9150
Reconstituted Complex Homo sapiens
33 SUPT7L  
Affinity Capture-Western Homo sapiens
34 ACVR1 90
Affinity Capture-MS Homo sapiens
35 CEBPE  
Two-hybrid Homo sapiens
36 NTRK1 4914
Affinity Capture-MS Homo sapiens
37 MED26  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 SUPT3H  
Affinity Capture-Western Homo sapiens
39 MED25  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 HECTD1 25831
Affinity Capture-MS Homo sapiens
41 LZTR1 8216
Two-hybrid Homo sapiens
42 MED31  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 CEBPA  
Protein-peptide Homo sapiens
44 MED15  
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
45 POLR2B 5431
Affinity Capture-MS Homo sapiens
46 HNRNPD 3184
Affinity Capture-MS Homo sapiens
47 HIST1H3A 8350
Affinity Capture-MS Homo sapiens
48 CCNC  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 BAG2 9532
Affinity Capture-MS Homo sapiens
50 EPAS1  
Affinity Capture-MS Homo sapiens
51 ACAD11 84129
Affinity Capture-MS Homo sapiens
52 CDK19 23097
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 EGFR 1956
Negative Genetic Homo sapiens
54 OBFC1 79991
Affinity Capture-MS Homo sapiens
55 FOSL2  
Affinity Capture-MS Homo sapiens
56 MYO1C 4641
Affinity Capture-MS Homo sapiens
57 THRA  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
58 HNF4A  
Reconstituted Complex Homo sapiens
59 MED10  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 TADA2A  
Affinity Capture-MS Homo sapiens
61 MYC  
Reconstituted Complex Homo sapiens
62 HECW2  
Affinity Capture-MS Homo sapiens
63 EMC2 9694
Affinity Capture-MS Homo sapiens
64 MED23 9439
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 Junb  
Affinity Capture-MS Mus musculus
66 Rpl35 66489
Affinity Capture-MS Mus musculus
67 EFNB2 1948
Affinity Capture-MS Homo sapiens
68 MED14  
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
69 ZC3H13 23091
Affinity Capture-MS Homo sapiens
70 MED11  
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 MED19  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
73 ESR2  
Reconstituted Complex Homo sapiens
74 SIRT6  
Affinity Capture-MS Homo sapiens
75 KAT2A  
Affinity Capture-Western Homo sapiens
76 TP53 7157
Affinity Capture-MS Homo sapiens
77 CDK8 1024
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 OPALIN  
Affinity Capture-MS Homo sapiens
79 WBP11  
Co-fractionation Homo sapiens
80 MED8  
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
81 MED6  
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
82 DDX58 23586
Affinity Capture-RNA Homo sapiens
83 ESR1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
84 MED30  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
85 MED18  
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
86 MED21  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
87 TCL1B  
Affinity Capture-MS Homo sapiens
88 MED12  
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
89 AR 367
Proximity Label-MS Homo sapiens
90 PPARGC1A  
Affinity Capture-Western Homo sapiens
91 MED28  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which MED16 is involved
PathwayEvidenceSource
Adipogenesis TAS Reactome
Adipogenesis IEA Reactome
Developmental Biology TAS Reactome
Developmental Biology IEA Reactome
Disease TAS Reactome
Epigenetic regulation by WDR5-containing histone modifying complexes TAS Reactome
Epigenetic regulation by WDR5-containing histone modifying complexes IEA Reactome
Epigenetic regulation of adipogenesis genes by MLL3 and MLL4 complexes TAS Reactome
Epigenetic regulation of adipogenesis genes by MLL3 and MLL4 complexes IEA Reactome
Epigenetic regulation of gene expression TAS Reactome
Epigenetic regulation of gene expression IEA Reactome
Epigenetic regulation of gene expression by MLL3 and MLL4 complexes TAS Reactome
Epigenetic regulation of gene expression by MLL3 and MLL4 complexes IEA Reactome
Gene expression (Transcription) TAS Reactome
Gene expression (Transcription) IEA Reactome
Generic Transcription Pathway TAS Reactome
Infectious disease TAS Reactome
Metabolism TAS Reactome
Metabolism of lipids TAS Reactome
MLL4 and MLL3 complexes regulate expression of PPARG target genes in adipogenesis and hepatic steatosis TAS Reactome
MLL4 and MLL3 complexes regulate expression of PPARG target genes in adipogenesis and hepatic steatosis IEA Reactome
PPARA activates gene expression TAS Reactome
Regulation of lipid metabolism by PPARalpha TAS Reactome
Respiratory Syncytial Virus Infection Pathway TAS Reactome
RNA Polymerase II Transcription TAS Reactome
RSV-host interactions TAS Reactome
Transcriptional regulation of white adipocyte differentiation TAS Reactome
Transcriptional regulation of white adipocyte differentiation IEA Reactome
Viral Infection Pathways TAS Reactome





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