Experiment description of studies that identified H1FX in exosomes

1 Experiment ID 497 MISEV standards ✘ Biophysical techniques ✔ CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35931686     Organism Homo sapiens Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." Journal name Cell Death Dis Publication year 2022 Sample Chondrocytes Sample name T/C-28a2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

2 Experiment ID 498 MISEV standards ✔ EM Biophysical techniques ✔ CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35931686     Organism Homo sapiens Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." Journal name Cell Death Dis Publication year 2022 Sample Chondrocytes Sample name T/C-28a2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

3 Experiment ID 224 MISEV standards ✔ EM|AFM Biophysical techniques ✔ Alix|TSG101|CD63|CD81 Enriched markers ✔ GOLGA2 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25944692     Organism Homo sapiens Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S" Journal name Oncotarget Publication year 2015 Sample Neuroblastoma cells Sample name SH-SY5Y Isolation/purification methods Differential centrifugation Ultracentrifugation OptiPrep density gradient Flotation density 1.10 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting

4 Experiment ID 211 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|EpCAM|TFRC Enriched markers ✔ cytochrome c|GOLGA2 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23333927     Organism Homo sapiens Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes. Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K." Journal name J Proteomics Publication year 2013 Sample Ovarian cancer cells Sample name IGROV1 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.09-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

5 Experiment ID 212 MISEV standards ✔ CEM Biophysical techniques ✔ TSG101|Alix|EpCAM|TFRC Enriched markers ✔ Cytochrome C|GOLGA2 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23333927     Organism Homo sapiens Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes. Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K." Journal name J Proteomics Publication year 2013 Sample Ovarian cancer cells Sample name OVCAR-3 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.09-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

6 Experiment ID 834 MISEV standards ✔ EM Biophysical techniques ✔ LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

7 Experiment ID 835 MISEV standards ✔ EM Biophysical techniques ✔ CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

8 Experiment ID 217 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|CD81|CD9|CD63 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23844026     Organism Homo sapiens Experiment description Characterization of human thymic exosomes. Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O." Journal name PLoS One Publication year 2013 Sample Thymus Sample name Normal-Thymus Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

Protein-protein interactions for H1FX

Protein Interactor ExoCarta ID Identification method PubMed Species 1 POLR2B 5431 Co-fractionation Homo sapiens 2 SRP19 6728 Affinity Capture-MS Homo sapiens 3 HIST2H2BE 8349 Affinity Capture-MS Homo sapiens 4 UBE2H 7328 Affinity Capture-MS Homo sapiens 5 SPRTN   Affinity Capture-MS Homo sapiens 6 HIST1H2BH 8345 Cross-Linking-MS (XL-MS) Homo sapiens 7 PAXIP1   Affinity Capture-MS Homo sapiens 8 EIF2S3 1968 Affinity Capture-MS Homo sapiens 9 HIST1H4A 8359 Affinity Capture-MS Homo sapiens 10 IPO7 10527 Affinity Capture-MS Homo sapiens 11 CALU 813 Co-fractionation Homo sapiens 12 TARDBP 23435 Affinity Capture-MS Homo sapiens 13 HIST1H1D 3007 Affinity Capture-MS Homo sapiens 14 KPNB1 3837 Affinity Capture-MS Homo sapiens 15 URB1 9875 Cross-Linking-MS (XL-MS) Homo sapiens 16 EBNA-LP   Affinity Capture-MS 17 NOP56 10528 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 18 RPS19 6223 Affinity Capture-MS Homo sapiens 19 RPL10 6134 Affinity Capture-MS Homo sapiens 20 BRD2   Affinity Capture-MS Homo sapiens 21 MAP4K4 9448 Affinity Capture-MS Homo sapiens 22 EIF4A1 1973 Cross-Linking-MS (XL-MS) Homo sapiens 23 CAND1 55832 Affinity Capture-MS Homo sapiens 24 Ktn1   Affinity Capture-MS Mus musculus 25 SOX2   Affinity Capture-MS Homo sapiens 26 KDM2A   Affinity Capture-MS Homo sapiens 27 CENPA   Proximity Label-MS Homo sapiens 28 USP36   Affinity Capture-MS Homo sapiens 29 Rrp1b 72462 Affinity Capture-MS Mus musculus 30 DIMT1 27292 Affinity Capture-MS Homo sapiens 31 METAP2 10988 Affinity Capture-MS Homo sapiens 32 SNRNP70 6625 Co-fractionation Homo sapiens 33 PGK1 5230 Cross-Linking-MS (XL-MS) Homo sapiens 34 B3GNT2 10678 Affinity Capture-MS Homo sapiens 35 TM9SF4 9777 Affinity Capture-MS Homo sapiens 36 DYRK1A 1859 Affinity Capture-MS Homo sapiens 37 MYO1E 4643 Affinity Capture-MS Homo sapiens 38 EPN2 22905 Affinity Capture-MS Homo sapiens 39 RRP12 23223 Co-fractionation Homo sapiens 40 EIF3B 8662 Affinity Capture-MS Homo sapiens 41 FBXW7   Affinity Capture-MS Homo sapiens 42 PRC1 9055 Affinity Capture-MS Homo sapiens 43 NPM1 4869 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 44 CALM3 808 Affinity Capture-MS Homo sapiens 45 HIST1H2AG 8969 Cross-Linking-MS (XL-MS) Homo sapiens 46 OBSL1 23363 Affinity Capture-MS Homo sapiens 47 HMGA1 3159 Affinity Capture-MS Homo sapiens 48 GSPT1 2935 Affinity Capture-MS Homo sapiens 49 EXOSC4 54512 Co-fractionation Homo sapiens 50 EED   Affinity Capture-MS Homo sapiens 51 SRP68 6730 Affinity Capture-MS Homo sapiens 52 DNAJC8 22826 Affinity Capture-MS Homo sapiens 53 DDX6 1656 Affinity Capture-MS Homo sapiens 54 Eif3i 54709 Affinity Capture-MS Mus musculus 55 METTL18   Affinity Capture-MS Homo sapiens 56 CUL1 8454 Affinity Capture-MS Homo sapiens 57 ARIH2 10425 Affinity Capture-MS Homo sapiens 58 NCK1 4690 Affinity Capture-MS Homo sapiens 59 SOD1 6647 Affinity Capture-MS Homo sapiens 60 RPL19 6143 Affinity Capture-MS Homo sapiens 61 CTCF   Affinity Capture-MS Homo sapiens 62 TPT1 7178 Affinity Capture-MS Homo sapiens 63 PDZD8 118987 Affinity Capture-MS Homo sapiens 64 KLF16   Proximity Label-MS Homo sapiens 65 SETD8   Affinity Capture-MS Homo sapiens 66 SRP9 6726 Affinity Capture-MS Homo sapiens 67 DLD 1738 Affinity Capture-MS Homo sapiens 68 NUMA1 4926 Affinity Capture-MS Homo sapiens 69 COX15 1355 Affinity Capture-MS Homo sapiens 70 DDX21 9188 Affinity Capture-MS Homo sapiens 71 RPL4 6124 Affinity Capture-MS Homo sapiens 72 RPS10 6204 Affinity Capture-MS Homo sapiens 73 HIST1H1B 3009 Co-fractionation Homo sapiens 74 MSX2   Affinity Capture-MS Homo sapiens 75 DOT1L 84444 Affinity Capture-MS Homo sapiens 76 DNAJC2 27000 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 77 VRK1 7443 Affinity Capture-MS Homo sapiens 78 RPL5 6125 Affinity Capture-MS Homo sapiens 79 RPL35 11224 Affinity Capture-MS Homo sapiens 80 PABPC1 26986 Affinity Capture-MS Homo sapiens 81 Srp72   Affinity Capture-MS Mus musculus 82 CHMP4B 128866 Affinity Capture-MS Homo sapiens 83 SREBF2 6721 Negative Genetic Homo sapiens 84 TRIM21 6737 Affinity Capture-MS Homo sapiens 85 NTRK1 4914 Affinity Capture-MS Homo sapiens 86 AIM2   Affinity Capture-MS Homo sapiens 87 IFI16 3428 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 88 RAD18   Affinity Capture-MS Homo sapiens 89 CHEK1   Affinity Capture-MS Homo sapiens 90 CDH1 999 Proximity Label-MS Homo sapiens 91 RPL36 25873 Affinity Capture-MS Homo sapiens 92 HIST1H1C 3006 Co-fractionation Homo sapiens Cross-Linking-MS (XL-MS) Homo sapiens 93 RPN1 6184 Cross-Linking-MS (XL-MS) Homo sapiens 94 FNBP1 23048 Affinity Capture-MS Homo sapiens 95 HECTD1 25831 Affinity Capture-MS Homo sapiens 96 ENY2 56943 Affinity Capture-MS Homo sapiens 97 DNM2 1785 Affinity Capture-MS Homo sapiens 98 RRP1B 23076 Affinity Capture-MS Homo sapiens 99 ACTC1 70 Proximity Label-MS Homo sapiens 100 NECAP1 25977 Affinity Capture-MS Homo sapiens 101 MYBBP1A 10514 Affinity Capture-MS Homo sapiens 102 Ksr1   Affinity Capture-MS Mus musculus 103 SEC61B 10952 Affinity Capture-MS Homo sapiens 104 CYLD   Affinity Capture-MS Homo sapiens 105 HMGB2 3148 Affinity Capture-MS Homo sapiens 106 RPL11 6135 Affinity Capture-MS Homo sapiens 107 RSL1D1 26156 Co-fractionation Homo sapiens 108 HNRNPD 3184 Affinity Capture-MS Homo sapiens 109 CYHR1   Co-fractionation Homo sapiens 110 ZBTB2 57621 Affinity Capture-MS Homo sapiens 111 RPL31 6160 Proximity Label-MS Homo sapiens 112 FANCD2   Affinity Capture-MS Homo sapiens 113 RC3H2   Affinity Capture-MS Homo sapiens 114 ECT2 1894 Affinity Capture-MS Homo sapiens 115 SIRT7   Affinity Capture-MS Homo sapiens 116 NCKAP1 10787 Affinity Capture-MS Homo sapiens 117 CTTN 2017 Affinity Capture-MS Homo sapiens 118 CUL3 8452 Affinity Capture-MS Homo sapiens 119 Rrbp1   Affinity Capture-MS Mus musculus 120 SRSF5 6430 Affinity Capture-MS Homo sapiens 121 EGFR 1956 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 122 H1F0 3005 Affinity Capture-MS Homo sapiens 123 HNRNPA1 3178 Affinity Capture-MS Homo sapiens 124 MYC   Affinity Capture-MS Homo sapiens 125 SUZ12   Affinity Capture-MS Homo sapiens 126 XBP1   Two-hybrid Homo sapiens 127 HIST1H2BJ 8970 Cross-Linking-MS (XL-MS) Homo sapiens 128 MYCN   Affinity Capture-MS Homo sapiens 129 CEP250 11190 Affinity Capture-MS Homo sapiens 130 PIP4K2A 5305 Cross-Linking-MS (XL-MS) Homo sapiens 131 SOAT1 6646 Affinity Capture-MS Homo sapiens 132 IL7R   Protein-RNA Homo sapiens 133 FAM90A1   Affinity Capture-MS Homo sapiens 134 PSPC1 55269 Affinity Capture-MS Homo sapiens 135 RPA3 6119 Proximity Label-MS Homo sapiens 136 APEH 327 Co-fractionation Homo sapiens 137 DLST 1743 Affinity Capture-MS Homo sapiens 138 EPN1 29924 Affinity Capture-MS Homo sapiens 139 TOP1 7150 Affinity Capture-MS Homo sapiens 140 PRPF40A 55660 Co-fractionation Homo sapiens 141 RPL23A 6147 Affinity Capture-MS Homo sapiens 142 DDRGK1 65992 Affinity Capture-MS Homo sapiens 143 RNF2   Affinity Capture-MS Homo sapiens 144 BMI1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 145 VDAC1 7416 Affinity Capture-MS Homo sapiens 146 SLC25A6 293 Cross-Linking-MS (XL-MS) Homo sapiens 147 HIST1H2BC 8347 Cross-Linking-MS (XL-MS) Homo sapiens 148 BMP4 652 Affinity Capture-MS Homo sapiens 149 C9orf72   Affinity Capture-MS Homo sapiens 150 PYHIN1   Affinity Capture-MS Homo sapiens 151 ILF3 3609 Affinity Capture-MS Homo sapiens 152 RPS16 6217 Affinity Capture-MS Homo sapiens 153 RPL13 6137 Affinity Capture-MS Homo sapiens 154 DNAH6 1768 Cross-Linking-MS (XL-MS) Homo sapiens 155 ARRB2 409 Affinity Capture-MS Homo sapiens 156 PLEKHA4 57664 Affinity Capture-MS Homo sapiens 157 BKRF1   Affinity Capture-MS 158 HDGF 3068 Affinity Capture-MS Homo sapiens 159 HIST1H2BK 85236 Cross-Linking-MS (XL-MS) Homo sapiens 160 NMI   Co-fractionation Homo sapiens 161 MNDA 4332 Affinity Capture-MS Homo sapiens 162 UVSSA   Affinity Capture-MS Homo sapiens 163 HNRNPA2B1 3181 Cross-Linking-MS (XL-MS) Homo sapiens 164 PDHA1 5160 Affinity Capture-MS Homo sapiens 165 HMGN5 79366 Affinity Capture-MS Homo sapiens 166 VPS26A 9559 Co-fractionation Homo sapiens 167 SRP72 6731 Affinity Capture-MS Homo sapiens 168 CAPRIN1 4076 Affinity Capture-MS Homo sapiens 169 CAPZB 832 Affinity Capture-MS Homo sapiens 170 G3BP2 9908 Affinity Capture-MS Homo sapiens 171 H2AFB3   Affinity Capture-MS Homo sapiens 172 DDX58 23586 Affinity Capture-RNA Homo sapiens 173 ZEB1   Affinity Capture-MS Homo sapiens 174 SHMT2 6472 Co-fractionation Homo sapiens 175 ESR1   Affinity Capture-MS Homo sapiens 176 DRG1 4733 Affinity Capture-MS Homo sapiens 177 UFL1 23376 Affinity Capture-MS Homo sapiens 178 KRR1 11103 Affinity Capture-MS Homo sapiens 179 COQ2   Affinity Capture-MS Homo sapiens 180 ZNF746   Affinity Capture-MS Homo sapiens 181 RPS24 6229 Proximity Label-MS Homo sapiens 182 NCL 4691 Affinity Capture-MS Homo sapiens Cross-Linking-MS (XL-MS) Homo sapiens 183 EIF4B 1975 Cross-Linking-MS (XL-MS) Homo sapiens 184 ARRB1 408 Affinity Capture-MS Homo sapiens 185 ATG4B 23192 Affinity Capture-MS Homo sapiens 186 RPL7A 6130 Affinity Capture-MS Homo sapiens 187 CBX8 57332 Affinity Capture-MS Homo sapiens 188 RBM8A 9939 Affinity Capture-MS Homo sapiens 189 CIT 11113 Affinity Capture-MS Homo sapiens 190 GRSF1 2926 Proximity Label-MS Homo sapiens 191 CUL7 9820 Affinity Capture-MS Homo sapiens 192 HNRNPU 3192 Affinity Capture-MS Homo sapiens 193 GNB2L1 10399 Affinity Capture-MS Homo sapiens 194 RC3H1 149041 Affinity Capture-MS Homo sapiens 195 TRIM29 23650 Affinity Capture-MS Homo sapiens 196 NUCKS1 64710 Affinity Capture-MS Homo sapiens 197 PRMT1 3276 Affinity Capture-MS Homo sapiens View the network

Pathways in which H1FX is involved

No pathways found