Gene description for ATG4B
Gene name autophagy related 4B, cysteine peptidase
Gene symbol ATG4B
Other names/aliases APG4B
AUTL1
Species Homo sapiens
 Database cross references - ATG4B
ExoCarta ExoCarta_23192
Entrez Gene 23192
HGNC 20790
MIM 611338
UniProt Q9Y4P1  
 ATG4B identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for ATG4B
Molecular Function
    protein binding GO:0005515 IPI
    endopeptidase activity GO:0004175 IDA
    cysteine-type peptidase activity GO:0008234 IDA
    cysteine-type endopeptidase activity GO:0004197 IBA
Biological Process
    autophagy GO:0006914 IGI
    protein targeting to membrane GO:0006612 IBA
    nucleophagy GO:0044804 IBA
    proteolysis GO:0006508 IDA
    protein delipidation GO:0051697 IBA
    C-terminal protein lipidation GO:0006501 IBA
    autophagosome assembly GO:0000045 IGI
    protein processing GO:0016485 IBA
    positive regulation of protein catabolic process GO:0045732 IEA
    mitochondrion degradation GO:0000422 IBA
    cellular response to nitrogen starvation GO:0006995 IBA
    positive regulation of autophagy GO:0010508 IDA
Subcellular Localization
    cytosol GO:0005829 IBA
    cytoplasm GO:0005737 ISS
 Experiment description of studies that identified ATG4B in exosomes
1
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for ATG4B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GABARAP  
Two-hybrid Homo sapiens
2 GABARAPL2 11345
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
3 C14orf139  
Two-hybrid Homo sapiens
4 MAP1LC3B 81631
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which ATG4B is involved
No pathways found





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