Gene description for DDX3Y
Gene name DEAD (Asp-Glu-Ala-Asp) box helicase 3, Y-linked
Gene symbol DDX3Y
Other names/aliases DBY
Species Homo sapiens
 Database cross references - DDX3Y
ExoCarta ExoCarta_8653
Entrez Gene 8653
HGNC 2699
MIM 400010
UniProt O15523  
 DDX3Y identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Prostate cancer cells 25844599    
 Gene ontology annotations for DDX3Y
Molecular Function
    ATP binding GO:0005524 IEA
    DNA binding GO:0003677 IEA
    ATP-dependent RNA helicase activity GO:0004004 IBA
    RNA binding GO:0003723 IEA
Biological Process
    RNA secondary structure unwinding GO:0010501 IBA
    chromosome segregation GO:0007059 IBA
    regulation of gene expression GO:0010468 IBA
    translational initiation GO:0006413 IBA
Subcellular Localization
    cytoplasmic ribonucleoprotein granule GO:0036464 IBA
    membrane GO:0016020 IDA
    nucleus GO:0005634 IEA
 Experiment description of studies that identified DDX3Y in exosomes
1
Experiment ID 224
ISEV standards
EM|AFM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81
EV Membrane markers
GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 275
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|RAB5A
EV Cytosolic markers
CD9|CD82|CD63|CD81
EV Membrane markers
AIF
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
 Protein-protein interactions for DDX3Y
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which DDX3Y is involved
No pathways found





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