Gene description for ATG3
Gene name autophagy related 3
Gene symbol ATG3
Other names/aliases APG3
APG3-LIKE
APG3L
PC3-96
Species Homo sapiens
 Database cross references - ATG3
ExoCarta ExoCarta_64422
Vesiclepedia VP_64422
Entrez Gene 64422
HGNC 20962
MIM 609606
UniProt Q9NT62  
 ATG3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for ATG3
Molecular Function
    protein binding GO:0005515 IPI
    Atg8-family ligase activity GO:0019776 ISS
    Atg12 transferase activity GO:0019777 ISS
    ubiquitin-like protein transferase activity GO:0019787 IDA
    enzyme binding GO:0019899 IPI
    Atg8-family conjugating enzyme activity GO:0141046 IBA
    Atg8-family conjugating enzyme activity GO:0141046 IDA
    Atg8-family conjugating enzyme activity GO:0141046 IMP
Biological Process
    autophagosome assembly GO:0000045 IBA
    autophagosome assembly GO:0000045 IMP
    autophagosome assembly GO:0000045 ISS
    autophagy of mitochondrion GO:0000422 IBA
    protein targeting to membrane GO:0006612 ISS
    protein transport GO:0015031 IEA
    protein ubiquitination GO:0016567 IDA
    mitochondrial fragmentation involved in apoptotic process GO:0043653 ISS
    nucleophagy GO:0044804 IBA
    negative regulation of phagocytosis GO:0050765 IEA
    glycophagy GO:0061723 IBA
    regulation of cilium assembly GO:1902017 ISS
Subcellular Localization
    phagophore assembly site GO:0000407 IBA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    Atg12-Atg5-Atg16 complex GO:0034274 IDA
 Experiment description of studies that identified ATG3 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ATG3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ATG10  
Affinity Capture-MS Homo sapiens
2 GABARAPL1 23710
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
3 BAG3 9531
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
4 HIST2H2BE 8349
Affinity Capture-MS Homo sapiens
5 MAP1LC3C  
Affinity Capture-MS Homo sapiens
6 ATG16L1 55054
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 ATG4B 23192
Affinity Capture-MS Homo sapiens
8 PRKCSH 5589
Co-fractionation Homo sapiens
9 FNBP1L 54874
Affinity Capture-Western Homo sapiens
10 Tubg1 103733
Affinity Capture-MS Mus musculus
11 HRAS 3265
Affinity Capture-MS Homo sapiens
12 CFLAR  
Affinity Capture-Western Homo sapiens
13 TTBK1  
Affinity Capture-MS Homo sapiens
14 ATG5 9474
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 Prkcz  
Affinity Capture-MS Mus musculus
16 AMBRA1  
Affinity Capture-MS Homo sapiens
17 XPO1 7514
Affinity Capture-MS Homo sapiens
18 EMC8 10328
Affinity Capture-MS Homo sapiens
19 KRT85  
Affinity Capture-MS Homo sapiens
20 NFKBID  
Affinity Capture-MS Homo sapiens
21 EFR3A 23167
Affinity Capture-MS Homo sapiens
22 MAP1S 55201
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 DFFA 1676
Co-fractionation Homo sapiens
24 DLD 1738
Affinity Capture-MS Homo sapiens
25 ATG12  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
26 EDEM1  
Affinity Capture-MS Homo sapiens
27 GABARAPL2 11345
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 FGB 2244
Affinity Capture-MS Homo sapiens
29 GNAQ 2776
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 NHLRC2 374354
Co-fractionation Homo sapiens
31 MAP1B 4131
Affinity Capture-MS Homo sapiens
32 ASS1 445
Co-fractionation Homo sapiens
33 NANS 54187
Co-fractionation Homo sapiens
34 CREBBP  
Affinity Capture-Western Homo sapiens
35 HIST1H2BD 3017
Affinity Capture-MS Homo sapiens
36 CLU 1191
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
37 TNFAIP8 25816
Co-fractionation Homo sapiens
38 ZNF414  
Affinity Capture-MS Homo sapiens
39 PDE2A  
Affinity Capture-MS Homo sapiens
40 HIST2H2AA3 8337
Affinity Capture-MS Homo sapiens
41 MAP1LC3B 81631
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
42 MAP1LC3A 84557
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
43 C2orf73  
Affinity Capture-MS Homo sapiens
44 PRMT1 3276
Affinity Capture-MS Homo sapiens
45 BECN1 8678
Reconstituted Complex Homo sapiens
46 GABARAP 11337
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
47 TECPR1 25851
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 RPL23 9349
Co-fractionation Homo sapiens
49 GPR141  
Affinity Capture-MS Homo sapiens
50 RPL35A 6165
Affinity Capture-MS Homo sapiens
51 HSPA1A 3303
Affinity Capture-MS Homo sapiens
52 SPANXN4  
Affinity Capture-MS Homo sapiens
53 EP300 2033
Affinity Capture-MS Homo sapiens
54 MOB2 81532
Two-hybrid Homo sapiens
55 Trappc11  
Affinity Capture-MS Mus musculus
56 SUB1 10923
Affinity Capture-MS Homo sapiens
57 ANKRD39  
Affinity Capture-MS Homo sapiens
58 OBSL1 23363
Affinity Capture-MS Homo sapiens
59 HSPA9 3313
Co-fractionation Homo sapiens
60 EN1  
Affinity Capture-MS Homo sapiens
61 ACTBL2 345651
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 CCNY 219771
Affinity Capture-MS Homo sapiens
63 IL1B  
Affinity Capture-MS Homo sapiens
64 HUWE1 10075
Affinity Capture-MS Homo sapiens
65 MAP1LC3B2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 ATG7 10533
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which ATG3 is involved
PathwayEvidenceSource
Autophagy TAS Reactome
Macroautophagy TAS Reactome





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