ExoCarta: Gene summary

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Gene description for EMC8
Gene name	ER membrane protein complex subunit 8
Gene symbol	EMC8
Other names/aliases	C16orf2 C16orf4 COX4NB FAM158B NOC4
Species	Homo sapiens
Database cross references - EMC8
ExoCarta	ExoCarta_10328
Vesiclepedia	VP_10328
Entrez Gene	10328
HGNC	7864
MIM	604886
UniProt	O43402
EMC8 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942

Gene ontology annotations for EMC8

Molecular Function
	protein binding	GO:0005515	IPI
	membrane insertase activity	GO:0032977	IMP
Biological Process
	protein insertion into ER membrane by stop-transfer membrane-anchor sequence	GO:0045050	IDA
	protein insertion into ER membrane by stop-transfer membrane-anchor sequence	GO:0045050	IMP
	tail-anchored membrane protein insertion into ER membrane	GO:0071816	IDA
Subcellular Localization
	cytoplasm	GO:0005737	IDA
	endoplasmic reticulum	GO:0005783	IDA
	endoplasmic reticulum membrane	GO:0005789	NAS
	Golgi apparatus	GO:0005794	IDA
	membrane	GO:0016020	HDA
	EMC complex	GO:0072546	IBA
	EMC complex	GO:0072546	IDA
	EMC complex	GO:0072546	IPI

Experiment description of studies that identified EMC8 in exosomes

Experiment ID

489

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 6

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

490

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 7

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

491

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 8

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

492

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 9

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Protein-protein interactions for EMC8

Protein Interactor

ExoCarta ID

Identification method

PubMed

Species

RNF149

284996

Affinity Capture-MS

Homo sapiens

EIF3A

8661

Affinity Capture-MS

Homo sapiens

PDHA1

5160

Co-fractionation

Homo sapiens

ATP6V1B2

526

Co-fractionation

Homo sapiens

HSD17B11

51170

Proximity Label-MS

Homo sapiens

CSGALNACT2

55454

Affinity Capture-MS

Homo sapiens

QARS

5859

Affinity Capture-MS

Homo sapiens

VAPA

9218

Affinity Capture-MS

Homo sapiens

METTL7A

25840

Proximity Label-MS

Homo sapiens

RPL30

6156

Affinity Capture-MS

Homo sapiens

KRT6A

3853

Affinity Capture-MS

Homo sapiens

APOE

348

Co-fractionation

Homo sapiens

GJD3

Proximity Label-MS

Homo sapiens

KARS

3735

Affinity Capture-MS

Homo sapiens

DNAJC25

548645

Proximity Label-MS

Homo sapiens

PTPN1

5770

Proximity Label-MS

Homo sapiens

STX4

6810

Proximity Label-MS

Homo sapiens

APP

351

Reconstituted Complex

Homo sapiens

RPS3

6188

Affinity Capture-MS

Homo sapiens

ATG13

9776

Affinity Capture-MS

Homo sapiens

POR

5447

Co-fractionation

Homo sapiens

B3GAT1

Proximity Label-MS

Homo sapiens

NDUFS3

4722

Co-fractionation

Homo sapiens

DARS

1615

Affinity Capture-MS

Homo sapiens

APOC3

345

Affinity Capture-MS

Homo sapiens

Emc3

Affinity Capture-MS

Mus musculus

RCN2

5955

Co-fractionation

Homo sapiens

EIF3B

8662

Affinity Capture-MS

Homo sapiens

TRDN

10345

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

RPL24

6152

Affinity Capture-MS

Homo sapiens

PARVA

55742

Affinity Capture-MS

Homo sapiens

SSR1

6745

Proximity Label-MS

Homo sapiens

DDOST

1650

Affinity Capture-MS

Homo sapiens

KTN1

3895

Affinity Capture-MS

Homo sapiens

CCDC47

57003

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

NDUFV1

4723

Co-fractionation

Homo sapiens

DHX9

1660

Affinity Capture-MS

Homo sapiens

NDUFS1

4719

Co-fractionation

Homo sapiens

PABPC4

8761

Affinity Capture-MS

Homo sapiens

RPS23

6228

Affinity Capture-MS

Homo sapiens

EMC1

23065

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

KIAA1715

80856

Proximity Label-MS

Homo sapiens

PXMP2

Proximity Label-MS

Homo sapiens

CHMP4C

92421

Affinity Capture-MS

Homo sapiens

ENPP6

133121

Affinity Capture-MS

Homo sapiens

RPS9

6203

Affinity Capture-MS

Homo sapiens

HTR3C

Affinity Capture-MS

Homo sapiens

PANX1

24145

Proximity Label-MS

Homo sapiens

EMC10

284361

Affinity Capture-MS

Homo sapiens

P4HA1

5033

Co-fractionation

Homo sapiens

EMD

2010

Proximity Label-MS

Homo sapiens

COPE

11316

Affinity Capture-MS

Homo sapiens

OR4N2

Affinity Capture-MS

Homo sapiens

MARS

4141

Affinity Capture-MS

Homo sapiens

DEFB106A

Affinity Capture-MS

Homo sapiens

MCM10

55388

Affinity Capture-MS

Homo sapiens

C12orf49

Affinity Capture-MS

Homo sapiens

VCP

7415

Co-fractionation

Homo sapiens

APOA2

336

Affinity Capture-MS

Homo sapiens

RPL31

6160

Affinity Capture-MS

Homo sapiens

HMOX2

3163

Co-fractionation

Homo sapiens

RPS12

6206

Affinity Capture-MS

Homo sapiens

SLC28A2

Affinity Capture-MS

Homo sapiens

CYC1

1537

Co-fractionation

Homo sapiens

UQCRC1

7384

Co-fractionation

Homo sapiens

RPN1

6184

Proximity Label-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

ZNF581

Affinity Capture-MS

Homo sapiens

RPS16

6217

Affinity Capture-MS

Homo sapiens

UQCRH

7388

Co-fractionation

Homo sapiens

AGPS

8540

Co-fractionation

Homo sapiens

RHOT2

89941

Proximity Label-MS

Homo sapiens

ELOVL5

60481

Proximity Label-MS

Homo sapiens

SEC61B

10952

Proximity Label-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

ATP6V1E1

529

Co-fractionation

Homo sapiens

RMDN3

55177

Proximity Label-MS

Homo sapiens

STAM

8027

Affinity Capture-MS

Homo sapiens

RPL11

6135

Affinity Capture-MS

Homo sapiens

REEP5

7905

Proximity Label-MS

Homo sapiens

CLEC5A

Affinity Capture-MS

Homo sapiens

SEC63

11231

Proximity Label-MS

Homo sapiens

DPF2

Affinity Capture-MS

Homo sapiens

RPL32

6161

Affinity Capture-MS

Homo sapiens

EMC3

55831

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

RPN2

6185

Proximity Label-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

CANX

821

Affinity Capture-MS		Homo sapiens
Co-fractionation		Homo sapiens

RPL23

9349

Affinity Capture-MS

Homo sapiens

LRRC59

55379

Proximity Label-MS		Homo sapiens
Co-fractionation		Homo sapiens

NDUFA9

4704

Affinity Capture-MS

Homo sapiens

UQCRC2

7385

Co-fractionation

Homo sapiens

UBQLN2

29978

Affinity Capture-MS

Homo sapiens

Emc6

Affinity Capture-MS

Mus musculus

ATG12

Affinity Capture-MS

Homo sapiens

BCAP31

10134

Proximity Label-MS

Homo sapiens

HSD3B7

80270

Proximity Label-MS

Homo sapiens

RPS8

6202

Affinity Capture-MS

Homo sapiens

FKBP8

23770

Proximity Label-MS

Homo sapiens

FBXO6

26270

Affinity Capture-MS

Homo sapiens

CORO1C

23603

Co-fractionation

Homo sapiens

LPAR1

1902

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

100

NXF1

10482

Affinity Capture-RNA

Homo sapiens

101

FAM186B

Affinity Capture-MS

Homo sapiens

102

TMED10

10972

Affinity Capture-MS

Homo sapiens

103

ATP2A1

487

Proximity Label-MS

Homo sapiens

104

FBXO2

26232

Affinity Capture-MS

Homo sapiens

105

EMC2

9694

Two-hybrid		Homo sapiens
Two-hybrid		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Two-hybrid		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Cross-Linking-MS (XL-MS)		Homo sapiens
Cross-Linking-MS (XL-MS)		Homo sapiens
Affinity Capture-MS		Homo sapiens
Co-fractionation		Homo sapiens
Affinity Capture-MS		Homo sapiens
Cross-Linking-MS (XL-MS)		Homo sapiens

106

ASPH

444

Co-fractionation

Homo sapiens

107

MATR3

9782

Affinity Capture-MS

Homo sapiens

108

GNRH1

Affinity Capture-MS

Homo sapiens

109

COPA

1314

Affinity Capture-MS

Homo sapiens

110

WDR44

54521

Affinity Capture-MS

Homo sapiens

111

LMNA

4000

Proximity Label-MS

Homo sapiens

112

IFNA21

Affinity Capture-MS

Homo sapiens

113

KCNK16

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

114

OST4

Affinity Capture-MS

Homo sapiens

115

AIMP1

9255

Affinity Capture-MS

Homo sapiens

116

MTA2

9219

Affinity Capture-MS

Homo sapiens

117

EMC6

83460

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

118

PHB2

11331

Co-fractionation

Homo sapiens

119

Emc1

230866

Affinity Capture-MS

Mus musculus

120

SLC18A1

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

121

RAD17

Affinity Capture-MS

Homo sapiens

122

PGRMC1

10857

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

123

LONP1

9361

Co-fractionation

Homo sapiens

124

EMC7

56851

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

125

LRPPRC

10128

Co-fractionation

Homo sapiens

126

SAA1

6288

Affinity Capture-MS

Homo sapiens

127

PRRC2C

23215

Affinity Capture-MS

Homo sapiens

128

OR1A2

Affinity Capture-MS

Homo sapiens

129

BCKDHA

593

Co-fractionation

Homo sapiens

130

EMC4

51234

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Co-fractionation		Homo sapiens
Affinity Capture-MS		Homo sapiens

131

ATG101

Affinity Capture-MS

Homo sapiens

132

NDUFS7

374291

Co-fractionation

Homo sapiens

133

PDHB

5162

Co-fractionation

Homo sapiens

134

PSMD3

5709

Affinity Capture-MS

Homo sapiens

135

ATP2A2

488

Co-fractionation		Homo sapiens
Affinity Capture-MS		Homo sapiens

136

MMGT1

93380

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

137

EEF2

1938

Co-fractionation

Homo sapiens

138

NNT

23530

Co-fractionation

Homo sapiens

139

ERGIC2

51290

Proximity Label-MS

Homo sapiens

140

NDUFS8

4728

Co-fractionation

Homo sapiens

141

RPL38

6169

Affinity Capture-MS

Homo sapiens

142

MOV10

4343

Affinity Capture-RNA

Homo sapiens

143

SEC62

7095

Proximity Label-MS

Homo sapiens

144

ATP5B

506

Co-fractionation

Homo sapiens

145

RAB7A

7879

Proximity Label-MS

Homo sapiens

146

RPS7

6201

Affinity Capture-MS

Homo sapiens

147

STT3B

201595

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

148

DNAJC1

64215

Proximity Label-MS

Homo sapiens

149

RARS

5917

Affinity Capture-MS

Homo sapiens

150

CKAP4

10970

Proximity Label-MS

Homo sapiens

151

DERL1

79139

Proximity Label-MS

Homo sapiens

152

PRMT1

3276

Affinity Capture-MS

Homo sapiens

153

BCL7A

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

154

ATG3

64422

Affinity Capture-MS

Homo sapiens

155

S1PR4

Affinity Capture-MS

Homo sapiens

156

RPS13

6207

Affinity Capture-MS

Homo sapiens

View the network

Pathways in which EMC8 is involved
No pathways found

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