Gene description for LRPPRC
Gene name leucine-rich pentatricopeptide repeat containing
Gene symbol LRPPRC
Other names/aliases CLONE-23970
GP130
LRP130
LSFC
Species Homo sapiens
 Database cross references - LRPPRC
ExoCarta ExoCarta_10128
Entrez Gene 10128
HGNC 15714
MIM 607544
UniProt P42704  
 LRPPRC identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 17956143    
Hepatocytes 26054723    
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for LRPPRC
Molecular Function
    microtubule binding GO:0008017 TAS
    single-stranded DNA binding GO:0003697 IEA
    poly(A) RNA binding GO:0044822 IDA
    RNA binding GO:0003723 NAS
    actin filament binding GO:0051015 IDA
    protein binding GO:0005515 IPI
    ubiquitin protein ligase binding GO:0031625 IPI
    beta-tubulin binding GO:0048487 IDA
Biological Process
    mitochondrion transport along microtubule GO:0047497 TAS
    negative regulation of mitochondrial RNA catabolic process GO:0000961 IEA
    regulation of transcription, DNA-templated GO:0006355 IEA
    transcription, DNA-templated GO:0006351 IEA
    regulation of mitochondrial translation GO:0070129 IEA
    mRNA transport GO:0051028 IEA
Subcellular Localization
    nuclear inner membrane GO:0005637 IEA
    condensed nuclear chromosome GO:0000794 IDA
    microtubule GO:0005874 IDA
    membrane GO:0016020 IDA
    ribonucleoprotein complex GO:0030529 IEA
    mitochondrial nucleoid GO:0042645 IDA
    cytoskeleton GO:0005856 IDA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IEA
    perinuclear region of cytoplasm GO:0048471 IDA
    nuclear outer membrane GO:0005640 IEA
    mitochondrion GO:0005739 IDA
 Experiment description of studies that identified LRPPRC in exosomes
1
Experiment ID 20
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSP90
EV Cytosolic markers
✔
CD63|CD81|LAMP1
EV Membrane markers
✔
GOLGA2|cytochrome c
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17956143    
Organism Homo sapiens
Experiment description Proteomic analysis of microvesicles derived from human colorectal cancer cells.
Authors Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS
Journal name JPR
Publication year 2007
Sample Colorectal cancer cells
Sample name HT29
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Diafiltration
Flotation density 1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
2
Experiment ID 237
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
3
Experiment ID 224
ISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✔
GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
4
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for LRPPRC
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UXT  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
2 MAP1S 55201
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
3 MAGED1  
Affinity Capture-MS Homo sapiens
4 MAGEA6  
Affinity Capture-MS Homo sapiens
5 NEK6 10783
Affinity Capture-MS Homo sapiens
6 TNFRSF10D 8793
Affinity Capture-MS Homo sapiens
7 HEBP2 23593
Two-hybrid Homo sapiens
8 PTP4A3  
Affinity Capture-MS Homo sapiens
9 WDR8  
Affinity Capture-MS Homo sapiens
10 ARF6 382
Affinity Capture-MS Homo sapiens
11 CECR2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
12 GABARAPL2 11345
Affinity Capture-MS Homo sapiens
13 GSTK1 373156
Affinity Capture-MS Homo sapiens
14 PHLDA3  
Affinity Capture-MS Homo sapiens
15 IKBKE  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which LRPPRC is involved
PathwayEvidenceSource
Respiratory electron transport TAS Reactome
TP53 Regulates Metabolic Genes TAS Reactome





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