Gene description for NEK6
Gene name NIMA-related kinase 6
Gene symbol NEK6
Other names/aliases SID6-1512
Species Homo sapiens
 Database cross references - NEK6
ExoCarta ExoCarta_10783
Vesiclepedia VP_10783
Entrez Gene 10783
HGNC 7749
MIM 604884
UniProt Q9HC98  
 NEK6 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for NEK6
Molecular Function
    protein serine/threonine kinase activity GO:0004674 IDA
    protein binding GO:0005515 IPI
    signal transducer activity GO:0004871 IMP
    protein kinase binding GO:0019901 IPI
    magnesium ion binding GO:0000287 IDA
    kinesin binding GO:0019894 IPI
    ATP binding GO:0005524 IDA
Biological Process
    chromosome segregation GO:0007059 IDA
    positive regulation of I-kappaB kinase/NF-kappaB signaling GO:0043123 IMP
    protein autophosphorylation GO:0046777 IDA
    mitotic cell cycle GO:0000278 TAS
    mitotic nuclear division GO:0007067 IEA
    spindle assembly GO:0051225 TAS
    regulation of mitotic cell cycle GO:0007346 TAS
    protein phosphorylation GO:0006468 IDA
    peptidyl-serine phosphorylation GO:0018105 IDA
    regulation of cellular senescence GO:2000772 TAS
    regulation of mitotic metaphase/anaphase transition GO:0030071 IDA
    cytokinesis GO:0000910 TAS
    G2 DNA damage checkpoint GO:0031572 IMP
    apoptotic process GO:0006915 IEA
    mitotic nuclear envelope disassembly GO:0007077 TAS
    signal transduction GO:0007165 IMP
Subcellular Localization
    nuclear speck GO:0016607 IEA
    microtubule organizing center GO:0005815 IEA
    spindle pole GO:0000922 IEA
    cytoplasm GO:0005737 IDA
    nucleus GO:0005634 IDA
    microtubule GO:0005874 IEA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified NEK6 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 235
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Enriched markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for NEK6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HACD3 51495
Affinity Capture-MS Homo sapiens
2 MCM7 4176
Affinity Capture-MS Homo sapiens
3 SMC3 9126
Affinity Capture-MS Homo sapiens
4 EML3 256364
Affinity Capture-MS Homo sapiens
5 EML2 24139
Affinity Capture-MS Homo sapiens
6 RUVBL2 10856
Affinity Capture-MS Homo sapiens
7 EFCBP2  
Two-hybrid Homo sapiens
8 SLC1A5 6510
Affinity Capture-MS Homo sapiens
9 EML4 27436
Affinity Capture-MS Homo sapiens
10 USP9X 8239
Affinity Capture-MS Homo sapiens
11 FANCI 55215
Affinity Capture-MS Homo sapiens
12 CCAR2 57805
Affinity Capture-MS Homo sapiens
13 GCN1L1 10985
Affinity Capture-MS Homo sapiens
14 MYO1C 4641
Affinity Capture-MS Homo sapiens
15 KDELR1 10945
Affinity Capture-MS Homo sapiens
16 LRPPRC 10128
Affinity Capture-MS Homo sapiens
17 NEK7 140609
Affinity Capture-MS Homo sapiens
18 XPO5 57510
Affinity Capture-MS Homo sapiens
19 PSMD2 5708
Affinity Capture-MS Homo sapiens
20 IPO4 79711
Affinity Capture-MS Homo sapiens
21 PCID2 55795
Affinity Capture-MS Homo sapiens
22 DIPA  
Two-hybrid Homo sapiens
23 NUP93 9688
Affinity Capture-MS Homo sapiens
24 NEK9 91754
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
25 CDC37 11140
Affinity Capture-MS Homo sapiens
26 DYNLL1 8655
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which NEK6 is involved
PathwayEvidenceSource
Activation of NIMA Kinases NEK9, NEK6, NEK7 TAS Reactome
Nuclear Pore Complex (NPC) Disassembly TAS Reactome





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