Gene description for NEK9
Gene name NIMA-related kinase 9
Gene symbol NEK9
Other names/aliases NERCC
NERCC1
Nek8
Species Homo sapiens
 Database cross references - NEK9
ExoCarta ExoCarta_91754
Vesiclepedia VP_91754
Entrez Gene 91754
HGNC 18591
MIM 609798
UniProt Q8TD19  
 NEK9 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Embryonic kidney cells 36706192    
Embryonic kidney cells 36706192    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for NEK9
Molecular Function
    protein serine/threonine kinase activity GO:0004674 IDA
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    protein kinase binding GO:0019901 IBA
    protein kinase binding GO:0019901 IPI
    protein kinase activator activity GO:0030295 IDA
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    metal ion binding GO:0046872 IEA
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    mitotic cell cycle GO:0000278 IDA
    chromatin remodeling GO:0006338 IEA
    regulation of mitotic cell cycle GO:0007346 IDA
    cell division GO:0051301 IEA
Subcellular Localization
    nucleus GO:0005634 IEA
    centrosome GO:0005813 IBA
    centrosome GO:0005813 IDA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified NEK9 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 1275
MISEV standards
Biophysical techniques
✔
CD9|TSG101|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
Enriched markers
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 1280
MISEV standards
Biophysical techniques
✔
TSG101|CD9|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
Enriched markers
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T - Fraction 5
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density 1.151 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for NEK9
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 EML1 2009
Affinity Capture-MS Homo sapiens
2 UBE2H 7328
Affinity Capture-MS Homo sapiens
3 GABARAPL1 23710
Affinity Capture-MS Homo sapiens
4 BAG3 9531
Affinity Capture-MS Homo sapiens
5 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
6 MAFF 23764
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 SMC1A 8243
Affinity Capture-MS Homo sapiens
8 PRDM11  
Affinity Capture-MS Homo sapiens
9 FAM167A  
Affinity Capture-MS Homo sapiens
10 TP53 7157
Affinity Capture-MS Homo sapiens
11 AMBRA1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 XPO1 7514
Affinity Capture-MS Homo sapiens
13 NEK6 10783
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
14 PAK4 10298
Affinity Capture-MS Homo sapiens
15 C15orf59  
Affinity Capture-MS Homo sapiens
16 DNAJA2 10294
Affinity Capture-MS Homo sapiens
17 MCM5 4174
Co-fractionation Homo sapiens
18 AURKA 6790
Affinity Capture-MS Homo sapiens
19 CAP1 10487
Co-fractionation Homo sapiens
20 FAM96A  
Affinity Capture-MS Homo sapiens
21 ACTC1 70
Proximity Label-MS Homo sapiens
22 RNF19B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 MAP1LC3B 81631
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
24 GABARAPL2 11345
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 CUL3 8452
Affinity Capture-MS Homo sapiens
26 EML4 27436
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
27 CHEK1  
Affinity Capture-MS Homo sapiens
28 C9orf72  
Affinity Capture-MS Homo sapiens
29 PELI2 57161
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
30 SPDL1  
Co-fractionation Homo sapiens
31 HSP90AA1 3320
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-Western Homo sapiens
32 METTL21B  
Affinity Capture-MS Homo sapiens
33 Trappc8  
Affinity Capture-MS Mus musculus
34 NEK7 140609
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
35 TOP2A 7153
Affinity Capture-MS Homo sapiens
36 C2orf73  
Affinity Capture-MS Homo sapiens
37 C12orf74  
Affinity Capture-MS Homo sapiens
38 SF3B3 23450
Affinity Capture-MS Homo sapiens
39 TUBA1C 84790
Affinity Capture-MS Homo sapiens
40 MYC  
Affinity Capture-MS Homo sapiens
41 RPA3 6119
Proximity Label-MS Homo sapiens
42 SOHLH1  
Affinity Capture-MS Homo sapiens
43 RAN 5901
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
44 NTRK1 4914
Affinity Capture-MS Homo sapiens
45 KXD1 79036
Affinity Capture-MS Homo sapiens
46 EP300 2033
Affinity Capture-MS Homo sapiens
47 AMOT 154796
Proximity Label-MS Homo sapiens
48 Bag2  
Affinity Capture-MS Mus musculus
49 MDM2  
Affinity Capture-MS Homo sapiens
50 Dynll1 56455
Affinity Capture-MS Mus musculus
51 UBXN6 80700
Affinity Capture-MS Homo sapiens
52 AR 367
Affinity Capture-MS Homo sapiens
53 EDN3  
Affinity Capture-MS Homo sapiens
54 DYNLL2 140735
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 ATOH1  
Affinity Capture-MS Homo sapiens
56 SSRP1 6749
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
57 EPB41L5 57669
Affinity Capture-MS Homo sapiens
58 PRMT1 3276
Affinity Capture-MS Homo sapiens
59 REPIN1  
Co-fractionation Homo sapiens
60 NSMAF  
Affinity Capture-MS Homo sapiens
61 DYNLL1 8655
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 CUL4A 8451
Affinity Capture-MS Homo sapiens
63 HUWE1 10075
Affinity Capture-MS Homo sapiens
64 Nek9  
Affinity Capture-MS Mus musculus
65 GABARAP 11337
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
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