Gene description for NEK9
Gene name NIMA-related kinase 9
Gene symbol NEK9
Other names/aliases NERCC
NERCC1
Nek8
Species Homo sapiens
 Database cross references - NEK9
ExoCarta ExoCarta_91754
Entrez Gene 91754
HGNC 18591
MIM 609798
UniProt Q8TD19  
 NEK9 identified in exosomes derived from the following tissue/cell type
Thymus 23844026    
 Gene ontology annotations for NEK9
Molecular Function
    protein kinase binding GO:0019901 IPI
    metal ion binding GO:0046872 IEA
    protein binding GO:0005515 IPI
    protein serine/threonine kinase activity GO:0004674 TAS
    ATP binding GO:0005524 IEA
Biological Process
    mitotic nuclear division GO:0007067 IEA
    mitotic cell cycle GO:0000278 TAS
    protein phosphorylation GO:0006468 IBA
    cell division GO:0051301 IEA
    mitotic nuclear envelope disassembly GO:0007077 TAS
Subcellular Localization
    cytosol GO:0005829 TAS
    nucleus GO:0005634 IEA
 Experiment description of studies that identified NEK9 in exosomes
1
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for NEK9
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MBP  
Invitro Homo sapiens
2 NEK7 140609
Biochemical Activity Homo sapiens
3 GABARAPL2 11345
Affinity Capture-MS Homo sapiens
4 SSRP1 6749
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
5 BICD2  
Invivo Homo sapiens
Invitro Homo sapiens
6 RAN 5901
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
7 NEK6 10783
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
8 cdc2  
Biochemical Activity 8355
View the network image/svg+xml
 Pathways in which NEK9 is involved
PathwayEvidenceSource
Activation of NIMA Kinases NEK9, NEK6, NEK7 TAS Reactome
Nuclear Pore Complex (NPC) Disassembly TAS Reactome





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