|
 Gene description for NEK7 |
| Gene name |
NIMA-related kinase 7 |
| Gene symbol |
NEK7 |
| Other names/aliases |
- |
| Species |
Homo sapiens |
| Database cross references - NEK7 |
| ExoCarta |
ExoCarta_140609 |
| Entrez Gene |
140609 |
| HGNC |
13386 |
| MIM |
606848 |
| UniProt |
Q8TDX7
|
| NEK7 identified in exosomes derived from the following tissue/cell type |
|
Endothelial cells
|
26027894
|
|
Thymus
|
23844026
|
| Gene ontology annotations for NEK7 |
|
|
| Experiment description of studies that identified NEK7 in exosomes |
| 1 |
| Experiment ID |
226 |
| ISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
GAPDH
|
EV Cytosolic markers |
|
✔
CD9|FLOT1
|
EV Membrane markers |
|
✔
LMNA|H2AFX|ATP5A1|TOMM20
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
mRNA
|
| Identification method |
Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
|
| PubMed ID |
26027894
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting |
| Authors |
Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar |
| Journal name |
Journal of Extracellular Vesicles
|
| Publication year |
2015 |
| Sample |
Endothelial cells |
| Sample name |
HMEC-1 |
| Isolation/purification methods |
Differential ultracentrifugation
Sucrose density gradient |
| Flotation density |
1.10 g/mL
|
| Molecules identified in the study |
miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA |
| Methods used in the study |
Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting |
|
|
| 2 |
| Experiment ID |
217 |
| ISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
TSG101
|
EV Cytosolic markers |
|
✔
CD81|CD9|CD63
|
EV Membrane markers |
|
✘
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
23844026
|
| Organism |
Homo sapiens |
| Experiment description |
Characterization of human thymic exosomes. |
| Authors |
Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O. |
| Journal name |
PLoS One
|
| Publication year |
2013 |
| Sample |
Thymus |
| Sample name |
Normal-Thymus |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| Protein-protein interactions for NEK7 |
|
|
| Pathways in which NEK7 is involved |
|
|
|
