Gene description for PYCARD
Gene name PYD and CARD domain containing
Gene symbol PYCARD
Other names/aliases ASC
CARD5
TMS
TMS-1
TMS1
Species Homo sapiens
 Database cross references - PYCARD
ExoCarta ExoCarta_29108
Vesiclepedia VP_29108
Entrez Gene 29108
HGNC 16608
MIM 606838
UniProt Q9ULZ3  
 PYCARD identified in exosomes derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
 Gene ontology annotations for PYCARD
Molecular Function
    protein binding GO:0005515 IPI
    cysteine-type endopeptidase activator activity involved in apoptotic process GO:0008656 NAS
    protein homodimerization activity GO:0042803 IDA
    Pyrin domain binding GO:0032090 IPI
    ion channel binding GO:0044325 IEA
    BMP receptor binding GO:0070700 IPI
    myosin I binding GO:0017024 IPI
    enzyme binding GO:0019899 IPI
    protease binding GO:0002020 IPI
    interleukin-6 receptor binding GO:0005138 IPI
    tropomyosin binding GO:0005523 IPI
Biological Process
    tumor necrosis factor-mediated signaling pathway GO:0033209 IDA
    regulation of intrinsic apoptotic signaling pathway GO:2001242 IDA
    positive regulation of interleukin-6 secretion GO:2000778 IMP
    cellular response to tumor necrosis factor GO:0071356 IDA
    positive regulation of T cell migration GO:2000406 ISS
    positive regulation of antigen processing and presentation of peptide antigen via MHC class II GO:0002588 ISS
    positive regulation of release of cytochrome c from mitochondria GO:0090200 IDA
    defense response to Gram-negative bacterium GO:0050829 IMP
    positive regulation of NF-kappaB transcription factor activity GO:0051092 IDA
    positive regulation of cysteine-type endopeptidase activity GO:2001056 IDA
    positive regulation of JNK cascade GO:0046330 IMP
    positive regulation of apoptotic process GO:0043065 IDA
    negative regulation of protein serine/threonine kinase activity GO:0071901 IDA
    positive regulation of sequence-specific DNA binding transcription factor activity GO:0051091 IDA
    positive regulation of adaptive immune response GO:0002821 IMP
    positive regulation of defense response to virus by host GO:0002230 IEA
    interleukin-1 beta production GO:0032611 IEA
    positive regulation of chemokine secretion GO:0090197 IMP
    positive regulation of ERK1 and ERK2 cascade GO:0070374 IMP
    positive regulation of actin filament polymerization GO:0030838 ISS
    stimulatory C-type lectin receptor signaling pathway GO:0002223 TAS
    cellular response to interleukin-1 GO:0071347 IDA
    myeloid dendritic cell activation GO:0001773 IMP
    myeloid dendritic cell activation involved in immune response GO:0002277 ISS
    macropinocytosis GO:0044351 ISS
    cellular response to lipopolysaccharide GO:0071222 IDA
    positive regulation of activated T cell proliferation GO:0042104 ISS
    intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator GO:0042771 IMP
    positive regulation of interleukin-10 secretion GO:2001181 IMP
    regulation of tumor necrosis factor-mediated signaling pathway GO:0010803 IMP
    intrinsic apoptotic signaling pathway by p53 class mediator GO:0072332 IMP
    negative regulation of NF-kappaB transcription factor activity GO:0032088 IMP
    positive regulation of interleukin-1 beta secretion GO:0050718 IDA
    defense response to virus GO:0051607 IDA
    positive regulation of T cell activation GO:0050870 IMP
    positive regulation of interleukin-6 production GO:0032755 ISS
    inflammatory response GO:0006954 IEA
    positive regulation of phagocytosis GO:0050766 ISS
    regulation of autophagy GO:0010506 IEA
    regulation of protein stability GO:0031647 ISS
    apoptotic process GO:0006915 TAS
    negative regulation of interferon-beta production GO:0032688 IMP
    positive regulation of tumor necrosis factor production GO:0032760 IMP
    positive regulation of cysteine-type endopeptidase activity involved in apoptotic process GO:0043280 IDA
    innate immune response GO:0045087 TAS
    negative regulation of I-kappaB kinase/NF-kappaB signaling GO:0043124 IDA
    activation of innate immune response GO:0002218 IDA
    activation of cysteine-type endopeptidase activity involved in apoptotic process GO:0006919 NAS
    regulation of inflammatory response GO:0050727 IEA
    positive regulation of interferon-gamma production GO:0032729 ISS
    signal transduction GO:0007165 NAS
    positive regulation of extrinsic apoptotic signaling pathway GO:2001238 IDA
    nucleotide-binding domain, leucine rich repeat containing receptor signaling pathway GO:0035872 TAS
    positive regulation of interleukin-8 secretion GO:2000484 IMP
Subcellular Localization
    endoplasmic reticulum GO:0005783 IEA
    IkappaB kinase complex GO:0008385 TAS
    NLRP1 inflammasome complex GO:0072558 IDA
    cytoplasm GO:0005737 NAS
    nucleolus GO:0005730 IDA
    AIM2 inflammasome complex GO:0097169 IDA
    extracellular region GO:0005576 IEA
    NLRP3 inflammasome complex GO:0072559 IDA
    mitochondrion GO:0005739 IDA
    cytosol GO:0005829 TAS
    neuronal cell body GO:0043025 IEA
    nucleus GO:0005634 ISS
 Experiment description of studies that identified PYCARD in exosomes
1
Experiment ID 363
MISEV standards
✘ EV Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
EV Enriched markers
✔
DCLK1
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
✘ EV Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
EV Enriched markers
✔
DCLK1
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
✘ EV Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
EV Enriched markers
✔
DCLK1
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for PYCARD
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PRKAR1A 5573
Two-hybrid Homo sapiens
2 CARD12  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
3 MEFV  
Two-hybrid Homo sapiens
Invivo Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
4 SRF  
Invitro Homo sapiens
View the network image/svg+xml
 Pathways in which PYCARD is involved
PathwayEvidenceSource
CLEC7A/inflammasome pathway TAS Reactome
The AIM2 inflammasome TAS Reactome
The NLRP3 inflammasome TAS Reactome





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