Gene description for PYCARD
Gene name PYD and CARD domain containing
Gene symbol PYCARD
Other names/aliases ASC
CARD5
TMS
TMS-1
TMS1
Species Homo sapiens
 Database cross references - PYCARD
ExoCarta ExoCarta_29108
Vesiclepedia VP_29108
Entrez Gene 29108
HGNC 16608
MIM 606838
UniProt Q9ULZ3  
 PYCARD identified in exosomes derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
 Gene ontology annotations for PYCARD
Molecular Function
    protease binding GO:0002020 IPI
    interleukin-6 receptor binding GO:0005138 IPI
    protein binding GO:0005515 IPI
    tropomyosin binding GO:0005523 IPI
    cysteine-type endopeptidase activator activity involved in apoptotic process GO:0008656 NAS
    myosin I binding GO:0017024 IPI
    enzyme binding GO:0019899 IPI
    Pyrin domain binding GO:0032090 IPI
    pattern recognition receptor activity GO:0038187 IBA
    identical protein binding GO:0042802 IDA
    identical protein binding GO:0042802 IPI
    protein homodimerization activity GO:0042803 IDA
    transmembrane transporter binding GO:0044325 IEA
    protein dimerization activity GO:0046983 IDA
    BMP receptor binding GO:0070700 IPI
    cysteine-type endopeptidase activator activity GO:0140608 IBA
Biological Process
    myeloid dendritic cell activation GO:0001773 IMP
    activation of innate immune response GO:0002218 IBA
    activation of innate immune response GO:0002218 IDA
    pattern recognition receptor signaling pathway GO:0002221 NAS
    positive regulation of defense response to virus by host GO:0002230 IEA
    myeloid dendritic cell activation involved in immune response GO:0002277 ISS
    positive regulation of antigen processing and presentation of peptide antigen via MHC class II GO:0002588 ISS
    positive regulation of adaptive immune response GO:0002821 IMP
    apoptotic process GO:0006915 TAS
    inflammatory response GO:0006954 IBA
    signal transduction GO:0007165 NAS
    osmosensory signaling pathway GO:0007231 NAS
    regulation of autophagy GO:0010506 IEA
    regulation of tumor necrosis factor-mediated signaling pathway GO:0010803 IMP
    positive regulation of actin filament polymerization GO:0030838 ISS
    regulation of protein stability GO:0031647 ISS
    negative regulation of NF-kappaB transcription factor activity GO:0032088 IMP
    negative regulation of interferon-beta production GO:0032688 IMP
    positive regulation of chemokine production GO:0032722 IMP
    positive regulation of type II interferon production GO:0032729 ISS
    positive regulation of interleukin-1 beta production GO:0032731 IDA
    positive regulation of interleukin-1 beta production GO:0032731 IMP
    positive regulation of interleukin-1 beta production GO:0032731 ISO
    positive regulation of interleukin-1 beta production GO:0032731 NAS
    positive regulation of interleukin-10 production GO:0032733 IMP
    positive regulation of interleukin-6 production GO:0032755 IMP
    positive regulation of interleukin-6 production GO:0032755 ISS
    positive regulation of interleukin-8 production GO:0032757 IMP
    positive regulation of tumor necrosis factor production GO:0032760 IMP
    tumor necrosis factor-mediated signaling pathway GO:0033209 IDA
    positive regulation of activated T cell proliferation GO:0042104 ISS
    intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator GO:0042771 IMP
    positive regulation of apoptotic process GO:0043065 IDA
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 IDA
    negative regulation of canonical NF-kappaB signal transduction GO:0043124 IDA
    macropinocytosis GO:0044351 ISS
    NLRP3 inflammasome complex assembly GO:0044546 IDA
    innate immune response GO:0045087 IEA
    positive regulation of JNK cascade GO:0046330 IMP
    icosanoid biosynthetic process GO:0046456 NAS
    positive regulation of inflammatory response GO:0050729 IDA
    positive regulation of inflammatory response GO:0050729 ISO
    positive regulation of inflammatory response GO:0050729 NAS
    positive regulation of phagocytosis GO:0050766 ISS
    defense response to Gram-negative bacterium GO:0050829 IMP
    defense response to Gram-positive bacterium GO:0050830 IEA
    positive regulation of T cell activation GO:0050870 IMP
    positive regulation of DNA-binding transcription factor activity GO:0051091 IDA
    positive regulation of NF-kappaB transcription factor activity GO:0051092 IDA
    protein homooligomerization GO:0051260 IDA
    defense response to virus GO:0051607 IDA
    defense response to virus GO:0051607 NAS
    positive regulation of macrophage cytokine production GO:0060907 IEA
    pyroptotic inflammatory response GO:0070269 NAS
    positive regulation of ERK1 and ERK2 cascade GO:0070374 IMP
    cellular response to lipopolysaccharide GO:0071222 IDA
    cellular response to interleukin-1 GO:0071347 IDA
    cellular response to tumor necrosis factor GO:0071356 IDA
    negative regulation of protein serine/threonine kinase activity GO:0071901 IDA
    intrinsic apoptotic signaling pathway by p53 class mediator GO:0072332 IMP
    positive regulation of release of cytochrome c from mitochondria GO:0090200 IDA
    apoptotic signaling pathway GO:0097190 IMP
    intrinsic apoptotic signaling pathway GO:0097193 IBA
    negative regulation of cytokine production involved in inflammatory response GO:1900016 IMP
    positive regulation of non-canonical NF-kappaB signal transduction GO:1901224 IPI
    positive regulation of T cell migration GO:2000406 ISS
    positive regulation of cysteine-type endopeptidase activity GO:2001056 IDA
    positive regulation of cysteine-type endopeptidase activity GO:2001056 NAS
    positive regulation of extrinsic apoptotic signaling pathway GO:2001238 IDA
    regulation of intrinsic apoptotic signaling pathway GO:2001242 IDA
Subcellular Localization
    Golgi membrane GO:0000139 IDA
    extracellular region GO:0005576 TAS
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISS
    nucleoplasm GO:0005654 IDA
    nucleolus GO:0005730 IDA
    nucleolus GO:0005730 NAS
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 NAS
    mitochondrion GO:0005739 IDA
    endoplasmic reticulum GO:0005783 IEA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    microtubule GO:0005874 NAS
    IkappaB kinase complex GO:0008385 IDA
    IkappaB kinase complex GO:0008385 TAS
    protein-containing complex GO:0032991 IDA
    secretory granule lumen GO:0034774 TAS
    azurophil granule lumen GO:0035578 TAS
    neuronal cell body GO:0043025 IEA
    canonical inflammasome complex GO:0061702 IPI
    canonical inflammasome complex GO:0061702 NAS
    NLRP1 inflammasome complex GO:0072558 IDA
    NLRP1 inflammasome complex GO:0072558 IPI
    NLRP3 inflammasome complex GO:0072559 IBA
    NLRP3 inflammasome complex GO:0072559 IDA
    NLRP3 inflammasome complex GO:0072559 IPI
    AIM2 inflammasome complex GO:0097169 IDA
    AIM2 inflammasome complex GO:0097169 IPI
    NLRP6 inflammasome complex GO:0140738 IDA
 Experiment description of studies that identified PYCARD in exosomes
1
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for PYCARD
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PIGP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 DNAJC28  
Affinity Capture-MS Homo sapiens
3 NLRC4  
Two-hybrid Homo sapiens
4 FSTL5 56884
Affinity Capture-MS Homo sapiens
5 NAA15 80155
Affinity Capture-MS Homo sapiens
6 RALB 5899
Affinity Capture-Western Homo sapiens
7 PPP2R2B 5521
Affinity Capture-MS Homo sapiens
8 C18orf21  
Affinity Capture-MS Homo sapiens
9 EXOC5 10640
Two-hybrid Homo sapiens
10 AGPAT1 10554
Affinity Capture-MS Homo sapiens
11 NDUFA13 51079
Affinity Capture-MS Homo sapiens
12 RAB5A 5868
Affinity Capture-Western Homo sapiens
13 IL6ST 3572
Affinity Capture-MS Homo sapiens
14 FCF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 PYCARD 29108
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
16 TSHZ2  
Affinity Capture-MS Homo sapiens
17 ZYG11B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 FAM184A 79632
Affinity Capture-MS Homo sapiens
19 PRKAR1A 5573
Two-hybrid Homo sapiens
20 BRCA1 672
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
21 TMPRSS15  
Affinity Capture-MS Homo sapiens
22 TRAF6 7189
Co-localization Homo sapiens
23 MYO1C 4641
Affinity Capture-MS Homo sapiens
24 PAK1 5058
Affinity Capture-Western Homo sapiens
25 SQSTM1 8878
Affinity Capture-Western Homo sapiens
26 CDK15  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 SSBP2  
Affinity Capture-MS Homo sapiens
28 INPP4B 8821
Affinity Capture-MS Homo sapiens
29 MCM4 4173
Affinity Capture-MS Homo sapiens
30 NLRP2  
Affinity Capture-Western Homo sapiens
31 RIPK1 8737
Affinity Capture-Western Homo sapiens
32 MAP3K7  
Co-fractionation Homo sapiens
33 IKBKB 3551
Affinity Capture-Western Homo sapiens
34 TPM4 7171
Affinity Capture-MS Homo sapiens
35 MEFV  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
36 NEK7 140609
Affinity Capture-Western Homo sapiens
37 PSTPIP1 9051
Affinity Capture-MS Homo sapiens
38 CEP131 22994
Affinity Capture-MS Homo sapiens
39 NUP107 57122
Affinity Capture-MS Homo sapiens
40 CASP1  
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
41 TRIM28 10155
Affinity Capture-Western Homo sapiens
42 ARMC2  
Affinity Capture-MS Homo sapiens
43 BECN1 8678
Affinity Capture-Western Homo sapiens
44 KLK10 5655
Affinity Capture-MS Homo sapiens
45 NLRP3  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
46 AIM2  
Co-localization Homo sapiens
47 PIGT 51604
Affinity Capture-MS Homo sapiens
48 IFI16 3428
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
49 GOT1 2805
Affinity Capture-MS Homo sapiens
50 ZFYVE21  
Reconstituted Complex Homo sapiens
51 CCL21  
Affinity Capture-MS Homo sapiens
52 USP50  
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
53 SSUH2  
Affinity Capture-MS Homo sapiens
54 BMPR2 659
Affinity Capture-MS Homo sapiens
55 PML 5371
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
56 OR2A4 79541
Affinity Capture-MS Homo sapiens
57 Nlrp3  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
58 CUL2 8453
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 LEMD3  
Affinity Capture-MS Homo sapiens
60 CCR1  
Affinity Capture-MS Homo sapiens
61 LIG3 3980
Affinity Capture-MS Homo sapiens
62 CHUK 1147
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
63 DRC7  
Affinity Capture-MS Homo sapiens
64 STX17 55014
Affinity Capture-MS Homo sapiens
65 NFATC2IP  
Affinity Capture-MS Homo sapiens
66 PELI1 57162
Affinity Capture-Western Homo sapiens
67 BIRC5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 KIAA0226  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
69 HIF1A 3091
Affinity Capture-Western Homo sapiens
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