Gene description for IL6ST
Gene name interleukin 6 signal transducer
Gene symbol IL6ST
Other names/aliases CD130
CDW130
GP130
IL-6RB
Species Homo sapiens
 Database cross references - IL6ST
ExoCarta ExoCarta_3572
Vesiclepedia VP_3572
Entrez Gene 3572
HGNC 6021
MIM 600694
UniProt P40189  
 IL6ST identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells Unpublished / Not applicable
Mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for IL6ST
Molecular Function
    cytokine receptor activity GO:0004896 IBA
    ciliary neurotrophic factor receptor activity GO:0004897 IDA
    interleukin-6 receptor activity GO:0004915 IBA
    interleukin-6 receptor activity GO:0004915 IDA
    interleukin-11 receptor activity GO:0004921 IEA
    leukemia inhibitory factor receptor activity GO:0004923 IDA
    oncostatin-M receptor activity GO:0004924 IDA
    ciliary neurotrophic factor receptor binding GO:0005127 IPI
    interleukin-6 receptor binding GO:0005138 IPI
    protein binding GO:0005515 IPI
    coreceptor activity GO:0015026 IDA
    growth factor binding GO:0019838 IPI
    growth factor binding GO:0019838 IPI
    cytokine binding GO:0019955 IBA
    interleukin-11 binding GO:0019970 IEA
    interleukin-6 binding GO:0019981 IBA
    interleukin-6 binding GO:0019981 IPI
    protein tyrosine kinase activator activity GO:0030296 IDA
    identical protein binding GO:0042802 IPI
    interleukin-27 receptor activity GO:0045509 IC
    scaffold protein binding GO:0097110 IPI
Biological Process
    positive regulation of acute inflammatory response GO:0002675 IC
    positive regulation of adaptive immune response GO:0002821 IC
    glycogen metabolic process GO:0005977 IEA
    positive regulation of cell population proliferation GO:0008284 IBA
    positive regulation of cell population proliferation GO:0008284 IDA
    positive regulation of cell population proliferation GO:0008284 IGI
    positive regulation of vascular endothelial growth factor production GO:0010575 TAS
    positive regulation of cardiac muscle hypertrophy GO:0010613 TAS
    cytokine-mediated signaling pathway GO:0019221 IBA
    cytokine-mediated signaling pathway GO:0019221 IDA
    response to cytokine GO:0034097 IDA
    interleukin-11-mediated signaling pathway GO:0038154 IDA
    oncostatin-M-mediated signaling pathway GO:0038165 IMP
    positive regulation of T cell proliferation GO:0042102 IMP
    negative regulation of apoptotic process GO:0043066 TAS
    negative regulation of neuron apoptotic process GO:0043524 IMP
    positive regulation of osteoblast differentiation GO:0045669 IMP
    positive regulation of Notch signaling pathway GO:0045747 IDA
    positive regulation of astrocyte differentiation GO:0048711 IEA
    leukemia inhibitory factor signaling pathway GO:0048861 IDA
    intestinal epithelial cell development GO:0060576 IDA
    interleukin-6-mediated signaling pathway GO:0070102 IDA
    interleukin-6-mediated signaling pathway GO:0070102 IMP
    interleukin-6-mediated signaling pathway GO:0070102 ISS
    interleukin-6-mediated signaling pathway GO:0070102 TAS
    negative regulation of interleukin-6-mediated signaling pathway GO:0070104 IDA
    interleukin-27-mediated signaling pathway GO:0070106 IMP
    ciliary neurotrophic factor-mediated signaling pathway GO:0070120 IDA
    T-helper 17 cell lineage commitment GO:0072540 ISS
    cell surface receptor signaling pathway via STAT GO:0097696 IMP
    positive regulation of platelet aggregation GO:1901731 TAS
Subcellular Localization
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 IDA
    plasma membrane GO:0005886 IC
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    interleukin-6 receptor complex GO:0005896 IDA
    oncostatin-M receptor complex GO:0005900 IDA
    external side of plasma membrane GO:0009897 IBA
    membrane GO:0016020 HDA
    dendrite GO:0030425 IEA
    neuronal cell body GO:0043025 IEA
    receptor complex GO:0043235 IBA
    membrane raft GO:0045121 IDA
    extracellular exosome GO:0070062 HDA
    ciliary neurotrophic factor receptor complex GO:0070110 IDA
 Experiment description of studies that identified IL6ST in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Antibody array
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
6
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for IL6ST
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UPK2 7379
Affinity Capture-MS Homo sapiens
2 KCNIP1  
Two-hybrid Homo sapiens
3 SOCS3 9021
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
4 PTPN11 5781
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
5 ERBB2 2064
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
6 CLGN 1047
Affinity Capture-MS Homo sapiens
7 GRB2 2885
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
8 SHC1 6464
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
9 PRKCD 5580
Affinity Capture-Western Homo sapiens
10 PTPN6 5777
Affinity Capture-Western Homo sapiens
11 MAGEA11  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
12 PYCARD 29108
Affinity Capture-MS Homo sapiens
13 TLE1 7088
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
14 VAV1 7409
Reconstituted Complex Homo sapiens
15 UBQLN1 29979
Two-hybrid Homo sapiens
16 LGALS1 3956
Affinity Capture-MS Homo sapiens
17 PARM1  
Affinity Capture-MS Homo sapiens
18 HCK 3055
Affinity Capture-Western Homo sapiens
19 ERBB3 2065
Affinity Capture-Western Homo sapiens
20 PIK3R1 5295
Affinity Capture-Western Homo sapiens
21 DDX58 23586
Affinity Capture-RNA Homo sapiens
22 KCNIP3  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
23 MCAM 4162
Proximity Label-MS Homo sapiens
24 SGTB  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
25 CDH1 999
Proximity Label-MS Homo sapiens
26 OSM 5008
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 IL2RA  
Affinity Capture-MS Homo sapiens
28 JAK1 3716
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
29 TYK2 7297
Affinity Capture-Western Homo sapiens
30 FBXO2 26232
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
31 CDK9 1025
Reconstituted Complex Homo sapiens
32 VAMP5 10791
Affinity Capture-MS Homo sapiens
33 SGTA 6449
Two-hybrid Homo sapiens
34 OSMR 9180
Affinity Capture-Western Homo sapiens
35 AR 367
Affinity Capture-Western Homo sapiens
36 CNTFR 1271
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
37 ST14 6768
Affinity Capture-MS Homo sapiens
38 STAT3 6774
Affinity Capture-Western Homo sapiens
39 JAK2 3717
Affinity Capture-Western Homo sapiens
40 IL6R  
Affinity Capture-Western Homo sapiens
41 LIFR 3977
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
42 TMPRSS13  
Affinity Capture-MS Homo sapiens
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