Gene description for IL6ST
Gene name interleukin 6 signal transducer
Gene symbol IL6ST
Other names/aliases CD130
CDW130
GP130
IL-6RB
Species Homo sapiens
 Database cross references - IL6ST
ExoCarta ExoCarta_3572
Vesiclepedia VP_3572
Entrez Gene 3572
HGNC 6021
MIM 600694
UniProt P40189  
 IL6ST identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells Unpublished / Not applicable
Mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for IL6ST
Molecular Function
    interleukin-6 receptor binding GO:0005138 IPI
    leukemia inhibitory factor receptor activity GO:0004923 IDA
    interleukin-6 binding GO:0019981 IPI
    interleukin-11 binding GO:0019970 IEA
    interleukin-27 receptor activity GO:0045509 IC
    ciliary neurotrophic factor receptor activity GO:0004897 IDA
    oncostatin-M receptor activity GO:0004924 IDA
    protein homodimerization activity GO:0042803 TAS
    interleukin-11 receptor activity GO:0004921 IEA
    protein binding GO:0005515 IPI
    interleukin-6 receptor activity GO:0004915 IDA
    ciliary neurotrophic factor receptor binding GO:0005127 IPI
    growth factor binding GO:0019838 IPI
Biological Process
    oncostatin-M-mediated signaling pathway GO:0038165 IMP
    regulation of Notch signaling pathway GO:0008593 IEA
    glycogen metabolic process GO:0005977 IEA
    positive regulation of vascular endothelial growth factor production GO:0010575 TAS
    positive regulation of tyrosine phosphorylation of Stat1 protein GO:0042511 IMP
    positive regulation of cell proliferation GO:0008284 IGI
    response to cytokine GO:0034097 IDA
    cytokine-mediated signaling pathway GO:0019221 IDA
    interleukin-6-mediated signaling pathway GO:0070102 IMP
    ciliary neurotrophic factor-mediated signaling pathway GO:0070120 IDA
    interleukin-27-mediated signaling pathway GO:0070106 IMP
    positive regulation of acute inflammatory response GO:0002675 IC
    positive regulation of T cell proliferation GO:0042102 IMP
    positive regulation of astrocyte differentiation GO:0048711 IEA
    positive regulation of adaptive immune response GO:0002821 IC
    viral process GO:0016032 IEA
    negative regulation of apoptotic process GO:0043066 TAS
    negative regulation of interleukin-6-mediated signaling pathway GO:0070104 IDA
    positive regulation of tyrosine phosphorylation of Stat3 protein GO:0042517 IMP
    leukemia inhibitory factor signaling pathway GO:0048861 IDA
    positive regulation of osteoblast differentiation GO:0045669 IMP
    positive regulation of cardiac muscle hypertrophy GO:0010613 TAS
    interleukin-11-mediated signaling pathway GO:0038154 IEA
Subcellular Localization
    interleukin-6 receptor complex GO:0005896 IDA
    plasma membrane GO:0005886 TAS
    neuronal cell body GO:0043025 IEA
    dendrite GO:0030425 IEA
    external side of plasma membrane GO:0009897 IEA
    oncostatin-M receptor complex GO:0005900 IDA
    extracellular exosome GO:0070062 IDA
    membrane GO:0016020 IDA
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 IDA
    ciliary neurotrophic factor receptor complex GO:0070110 IDA
 Experiment description of studies that identified IL6ST in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 126
MISEV standards
✘ EV Biophysical techniques
✔
GAPDH
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Antibody array
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
6
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 834
MISEV standards
✔
EM
EV Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 835
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for IL6ST
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PTPN11 5781
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
2 PIK3CG  
Invivo Homo sapiens
3 SOCS3 9021
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
4 STAT3 6774
Invivo Homo sapiens
5 AR 367
Affinity Capture-Western Homo sapiens
6 CNTFR 1271
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
7 VAV1 7409
Invivo Homo sapiens
8 TLE1 7088
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
9 ERBB2 2064
Invivo Homo sapiens
10 OSM 5008
Invivo Homo sapiens
11 CTF1  
Invivo Homo sapiens
12 JAK1 3716
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
13 IL6R  
Affinity Capture-Western Homo sapiens
14 LIFR 3977
Affinity Capture-Western Homo sapiens
15 HCK 3055
Invivo Homo sapiens
16 CDK9 1025
Reconstituted Complex Homo sapiens
17 ERBB3 2065
Invivo Homo sapiens
18 PTPN6 5777
Invivo Homo sapiens
19 PRKCD 5580
Invivo Homo sapiens
20 TYK2 7297
Invivo Homo sapiens
View the network image/svg+xml
 Pathways in which IL6ST is involved
PathwayEvidenceSource
Interleukin-6 signaling TAS Reactome





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