Gene description for PTPN6
Gene name protein tyrosine phosphatase, non-receptor type 6
Gene symbol PTPN6
Other names/aliases HCP
HCPH
HPTP1C
PTP-1C
SH-PTP1
SHP-1
SHP-1L
SHP1
Species Homo sapiens
 Database cross references - PTPN6
ExoCarta ExoCarta_5777
Entrez Gene 5777
HGNC 9658
MIM 176883
UniProt P29350  
 PTPN6 identified in exosomes derived from the following tissue/cell type
B cells 20458337    
B cells 20458337    
B cells 20458337    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Thymus 23844026    
Urine 19056867    
 Gene ontology annotations for PTPN6
Molecular Function
    SH3 domain binding GO:0017124 IEA
    SH2 domain binding GO:0042169 IEA
    transmembrane receptor protein tyrosine phosphatase activity GO:0005001 IDA
    protein kinase binding GO:0019901 IPI
    phosphotyrosine binding GO:0001784 IEA
    protein tyrosine phosphatase activity GO:0004725 TAS
    protein binding GO:0005515 IPI
Biological Process
    abortive mitotic cell cycle GO:0033277 IEA
    cytokine-mediated signaling pathway GO:0019221 TAS
    blood coagulation GO:0007596 TAS
    negative regulation of peptidyl-tyrosine phosphorylation GO:0050732 IMP
    positive regulation of phosphatidylinositol 3-kinase signaling GO:0014068 IMP
    negative regulation of MAP kinase activity GO:0043407 IEA
    peptidyl-tyrosine phosphorylation GO:0018108 IDA
    apoptotic process GO:0006915 TAS
    negative regulation of T cell receptor signaling pathway GO:0050860 IEA
    positive regulation of cell proliferation GO:0008284 IMP
    negative regulation of B cell receptor signaling pathway GO:0050859 IEA
    JAK-STAT cascade involved in growth hormone signaling pathway GO:0060397 TAS
    negative regulation of humoral immune response mediated by circulating immunoglobulin GO:0002924 IEA
    peptidyl-tyrosine dephosphorylation GO:0035335 TAS
    cell differentiation GO:0030154 IDA
    regulation of B cell differentiation GO:0045577 IEA
    megakaryocyte development GO:0035855 IEA
    platelet aggregation GO:0070527 IEA
    leukocyte migration GO:0050900 TAS
    T cell costimulation GO:0031295 TAS
    regulation of release of sequestered calcium ion into cytosol GO:0051279 IEA
    positive regulation of cell adhesion mediated by integrin GO:0033630 IEA
    regulation of interferon-gamma-mediated signaling pathway GO:0060334 TAS
    negative regulation of T cell proliferation GO:0042130 IEA
    G-protein coupled receptor signaling pathway GO:0007186 TAS
    regulation of ERK1 and ERK2 cascade GO:0070372 IDA
    interferon-gamma-mediated signaling pathway GO:0060333 TAS
    regulation of G1/S transition of mitotic cell cycle GO:2000045 IMP
    negative regulation of cell proliferation GO:0008285 NAS
    regulation of type I interferon-mediated signaling pathway GO:0060338 TAS
    type I interferon signaling pathway GO:0060337 TAS
    B cell receptor signaling pathway GO:0050853 IEA
    cell proliferation GO:0008283 IDA
    protein dephosphorylation GO:0006470 IDA
    platelet formation GO:0030220 IEA
    natural killer cell mediated cytotoxicity GO:0042267 IEA
Subcellular Localization
    cytoplasm GO:0005737 IDA
    nucleolus GO:0005730 IDA
    cytosol GO:0005829 TAS
    membrane GO:0016020 TAS
    extracellular exosome GO:0070062 IDA
    alpha-beta T cell receptor complex GO:0042105 IEA
    nucleus GO:0005634 IDA
    cell-cell junction GO:0005911 IEA
 Experiment description of studies that identified PTPN6 in exosomes
1
Experiment ID 79
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
CD81|MHCII
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
2
Experiment ID 80
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
CD81|MHCII
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 81
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
CD81|MHCII
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
4
Experiment ID 231
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD63|CD9
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 232
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 233
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 63
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
AQP2
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for PTPN6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 BLNK  
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
2 SIGLEC11  
Invivo Homo sapiens
3 PAG1  
Reconstituted Complex Homo sapiens
4 VAV1 7409
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
5 EPOR  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
6 HOXA10  
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
7 CXCR4 7852
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
8 CEACAM1 634
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
9 STAT5B 6777
Invivo Homo sapiens
10 PRKCD 5580
Invivo Homo sapiens
Invitro Homo sapiens
11 CD22 933
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
12 IRS4 8471
Invitro Homo sapiens
13 LILRB4  
Invitro Homo sapiens
14 CD72  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
15 GAB2 9846
Affinity Capture-Western Homo sapiens
16 KIT 3815
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
17 TNFRSF1A 7132
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
18 INSR 3643
Affinity Capture-Western Homo sapiens
19 ABL1 25
Affinity Capture-Western Homo sapiens
20 CLEC4A  
Invitro Homo sapiens
Invivo Homo sapiens
21 EGFR 1956
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
22 CD33 945
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
23 ROS1  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
24 IL6ST 3572
Invivo Homo sapiens
25 LYN 4067
Reconstituted Complex Homo sapiens
26 CBL 867
Affinity Capture-Western Homo sapiens
27 IL4R  
Reconstituted Complex Homo sapiens
28 SIRPA 140885
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
29 DOK1 1796
Invivo Homo sapiens
30 PECAM1 5175
Invivo Homo sapiens
Invitro Homo sapiens
31 CSF2RB  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
32 CD5 921
Invivo Homo sapiens
33 IFNAR1  
Affinity Capture-MS Homo sapiens
34 JAK1 3716
Biochemical Activity Homo sapiens
35 TYK2 7297
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
36 LILRB2  
Invitro Homo sapiens
Invitro Homo sapiens
37 TREML1 340205
Affinity Capture-Western Homo sapiens
38 PILRA 29992
Invivo Homo sapiens
Invivo Homo sapiens
39 LCP2 3937
Affinity Capture-MS Homo sapiens
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
40 PTK2B 2185
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
41 ZAP70 7535
Invitro Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
42 SYK 6850
Invivo Homo sapiens
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
43 NOS1  
Affinity Capture-Western Homo sapiens
44 FCGR2B  
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
45 SLAMF6 114836
Invivo Homo sapiens
Invitro Homo sapiens
46 GHR  
Invivo Homo sapiens
47 PIK3R1 5295
Two-hybrid Homo sapiens
48 KLRC1  
Invitro Homo sapiens
Invivo Homo sapiens
49 LAIR1  
Affinity Capture-MS Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
50 C6orf25  
Affinity Capture-Western Homo sapiens
51 JAK2 3717
Affinity Capture-Western Homo sapiens
52 CAV1 857
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
53 LIFR 3977
Invivo Homo sapiens
54 GRB2 2885
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Invitro Homo sapiens
Invivo Homo sapiens
55 LCK 3932
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Biochemical Activity Homo sapiens
56 CTNND1 1500
Invivo Homo sapiens
Invitro Homo sapiens
57 BCR 613
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
58 SSTR2  
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
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